Hair follicle stem cell cultures reveal self‐organizing plasticity of stem cells and their progeny
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Understanding how complex tissues are formed, maintained, and regenerated through local growth, differentiation, and remodeling requires knowledge on how single-cell behaviors are coordinated on the population level. The self-renewing hair follicle, maintained by a distinct stem cell population, represents an excellent paradigm to address this question. A major obstacle in mechanistic understanding of hair follicle stem cell (HFSC) regulation has been the lack of a culture system that recapitulates HFSC behavior while allowing their precise monitoring and manipulation. Here, we establish an in vitro culture system based on a 3D extracellular matrix environment and defined soluble factors, which for the first time allows expansion and long-term maintenance of murine multipotent HFSCs in the absence of heterologous cell types. Strikingly, this scheme promotes de novo generation of HFSCs from non-HFSCs and vice versa in a dynamic self-organizing process. This bidirectional interconversion of HFSCs and their progeny drives the system into a population equilibrium state. Our study uncovers regulatory dynamics by which phenotypic plasticity of cells drives population-level homeostasis within a niche, and provides a discovery tool for studies on adult stem cell fate.
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α6+;CD34+ HFSCs grown in similar conditions did not form these structures and instead resulted in non-organized spheroid clusters.
“Notably, when α6+;CD34+ HFSCs were grown in similar conditions we did not observe the formation of these structures. These cultures resulted in non-organized spheroid clusters highly similar to those recently reported20 (Supplementary Fig. 1g).”
From Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro (2019) · cites this paper as [20]
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- Authors
- Chacón‐Martínez, C. A., Klose, M., Niemann, C., Glauche, I., & Wickström, S. A.
- Affiliations
- Max Planck Institute for Biology of Ageing (DE)
- Technische Universität Dresden (DE)
- University of Cologne (DE)
- Corresponding author
- Wickström, Sara A
- Venue
- The EMBO Journal
- Published
- 2016-12-09
- Volume
- 36
- Issue
- 2
- Pages
- 151-164
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC5242381
- OpenAlex
- W2560658407
- Cited by
- 89
- References
- 40
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 8 · 2023: 11 · 2022: 12 · 2021: 12 · 2020: 12 · 2019: 12 · 2018: 6 · 2017: 5 · 2016: 1
- FWCI
- 11.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
- Funders
- Max‐Planck‐Gesellschaft
- Max Planck Förderstiftung
- Deutsche Forschungsgemeinschaft (WI 4177, SFB 829)
- Bundesministerium für Bildung und Forschung (031A315)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- hair follicle stem cells, in vitro culture system, 3D extracellular matrix, stem cell self-renewal, phenotypic plasticity, stem cell fate, stem cell niche, multipotent stem cells, tissue regeneration
- MeSH terms
- Animals, Cell Differentiation, Mice, Inbred BALB C, Mice, Inbred C57BL, Organ Culture Techniques, Stem Cells, Hair Follicle, Cell Culture Techniques
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