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Cited by 310

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Characterization of the Limbal Epithelial Stem Cell Niche: Novel Imaging Techniques Permit In Vivo Observation and Targeted Biopsy of Limbal Epithelial Stem Cells

Open access
Shortt, Alex J., Secker, Genevieve A., Munro, Peter M., Khaw, Peng T., Tuft, Stephen J., Daniels, Julie T.·2007·Stem Cells
DOI 10.1634/stemcells.2006-0580
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Abstract

It is anticipated that stem cell (SC) therapy will enable the regeneration of diseased tissues and organs. Understanding SC niches is an essential step toward realizing this goal. By virtue of its optical transparency and physical separation of SC and transient amplifying cell compartments, the human cornea provides a unique opportunity to visualize and observe a population of adult stem cells, limbal epithelial stem cells (LESCs), in their niche environment. To date, the characteristics of the LESC niche have remained unclear. State-of-the-art imaging techniques were used to construct a three-dimensional (3D) view of the entire human corneal limbus and identify the structural characteristics of the LESC niche. Two distinct candidate LESC niche structures were identified. Cells within these structures express high levels of the putative limbal stem cell markers p63α and ABCG2; however, current methods cannot identify for certain which exact cells within this cell population are truly LESCs. These structures could be located and observed in vivo in normal human subjects, but not in patients with clinically diagnosed corneal LESC deficiency. The distribution of these structures around the corneal circumference is not uniform. Biopsies targeted to limbal regions rich in LESC niche structures yielded significantly higher numbers of LESCs in culture. Our findings demonstrate how adult stem cell niches can be identified and observed in vivo in humans and provide new biological insight into the importance of LESC niche structures in maintaining normal LESC function. Finally, the concept of targeted biopsy of adult SC niches improves stem cell yield and may prove to be essential for the successful development of novel adult stem cell therapies. Disclosure of potential conflicts of interest is found at the end of this article.

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Metadata

Authors
Shortt, A. J., Secker, G. A., Munro, P. M., Khaw, P. T., Tuft, S. J., & Daniels, J. T.
Venue
Stem Cells
Publisher
Oxford University Press (OUP)
Published
2007-03-01
Volume
25
Issue
6
Pages
1402-1409
ISSN
  • 1066-5099 (ISSN-L)
  • 1549-4918
DOI
10.1634/stemcells.2006-0580
OpenAlex
W2037477217
Cited by
310
References
35
FWCI
9.58
Type
journal article
Language
en
Open access
bronze

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