The application of the CRISPR-Cas system in antibiotic resistance
Open accessAbstract
The emergence and global epidemic of antimicrobial resistance (AMR) poses a serious threat to global public health in recent years. AMR genes are shared between bacterial pathogens mainly via horizontal gene transfer (HGT) on mobile genetic elements (MGEs), thereby accelerating the spread of antimicrobial resistance (AMR) and increasing the burden of drug resistance. There is an urgent need to develop new strategies to control bacterial infections and the spread of antimicrobial resistance. The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are an RNA-guided adaptive immune system in prokaryotes that recognizes and defends against invasive genetic elements such as phages and plasmids. Because of its specifically target and cleave DNA sequences encoding antibiotic resistance genes, CRISPR/Cas system has been developed into a new gene-editing tool for the prevention and control of bacterial drug resistance. CRISPR-Cas plays a potentially important role in controlling horizontal gene transfer and limiting the spread of antibiotic resistance. In this review, we will introduce the structure and working mechanism of CRISPR-Cas systems, followed by delivery strategies, and then focus on the relationship between antimicrobial resistance and CRISPR-Cas. Moreover, the challenges and prospects of this research field are discussed, thereby providing a reference for the prevention and control of the spread of antibiotic resistance.
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Some bZIP transcription factors can act as negative regulators under specific conditions, influencing drought stress responses through complex interactions with other signaling pathways.
“While bZIP transcription factors are generally associated with positive regulation, some bZIP factors can act as negative regulators under specific conditions, influencing drought stress responses through complex interactions with other signaling pathways (Tao et al., 2022).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Tao et al., 2022
The paper’s bacterial-biofilms section discusses CRISPR-Cas and antibiotic resistance, not bZIP transcription factors or drought responses. Searches for bZIP negative regulation and plant drought-stress signaling found no passage addressing the claim; the closest results were unrelated references. No page number is available in our copy. (LLM, conf=0.77)
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CRISPR-Cas Systems: Bridging Bacterial Immunity and Host Interactions
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Application of CRISPR-Cas System to Mitigate Superbug Infections
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Biotechnological Innovations to Combat Antimicrobial Resistance and Advance Global Health Equity
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Taking the temperature of the United States public regarding microbiome engineering
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Exploring the evolutionary links: Innate immunity in bacteria and eukaryotes
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CRISPR Genome Editing in Personalized Therapy for Oral and Maxillofacial Diseases: A Scoping Review
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CRISPR-Cas mediated targeting of resistance genes for combating ESKAPE pathogen infections: A Review
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Microbiome interactions: Acinetobacter baumannii biofilms as a co-factor in oral cancer progression
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Harnessing advances in mechanisms, detection, and strategies to combat antimicrobial resistance
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Nanocarrier-mediated CRISPR-Cas delivery: a novel approach against antibiotic-resistant superbugs
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Genetic Mechanism of Cassava Disease Resistance: From Traditional Breeding to CRISPR/Cas Application
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Genomic Insights into Bacterial Antimicrobial Resistance Transmission and Mitigation Strategies
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Advances in CRISPR-Cas systems for human bacterial disease
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Omics-Based Approaches in Developing Treatments Against Antimicrobial Resistance
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- Authors
- Tao, S., Chen, H., Li, N., & Liang, W.
- Affiliations
- Jiangsu University (CN)
- Bengbu Medical College (CN)
- Corresponding author
- Liang, Wei
- Venue
- Infection and Drug Resistance
- Publisher
- Informa UK Limited
- Published
- 2022-08
- Volume
- Volume 15
- Pages
- 4155-4168
- ISSN
- 1178-6973 (ISSN-L)
- PMC
- PMC9356603
- OpenAlex
- W4289260617
- Cited by
- 147
- References
- 150
- Citations by year
- 2026: 38 · 2025: 53 · 2024: 42 · 2023: 13 · 2022: 1
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Infection and Drug Resistance (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Funders
- Jiangsu Commission of Health (H2018073)
- Jiangsu University (BRA2019248, H2018073, SF2015, BK20191210)
- Government of Jiangsu Province (333 Project)
- Natural Science Foundation of Jiangsu Province (BK20191210, 333 Project)
- Lianyungang Science and Technology Bureau
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- CRISPR-Cas, antimicrobial resistance, antibiotic resistance genes, horizontal gene transfer, mobile genetic elements, CRISPR gene editing, bacterial adaptive immunity, plasmids, bacteriophages
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