Cited by 147CRISPR and Genetic EngineeringAntibiotic Resistance in BacteriaBacterial Genetics and Biotechnology

The application of the CRISPR-Cas system in antibiotic resistance

Open access
Tao, Shuan, Chen, Huimin, Li, Na, Liang, Wei·2022·Infection and Drug Resistance

Abstract

The emergence and global epidemic of antimicrobial resistance (AMR) poses a serious threat to global public health in recent years. AMR genes are shared between bacterial pathogens mainly via horizontal gene transfer (HGT) on mobile genetic elements (MGEs), thereby accelerating the spread of antimicrobial resistance (AMR) and increasing the burden of drug resistance. There is an urgent need to develop new strategies to control bacterial infections and the spread of antimicrobial resistance. The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are an RNA-guided adaptive immune system in prokaryotes that recognizes and defends against invasive genetic elements such as phages and plasmids. Because of its specifically target and cleave DNA sequences encoding antibiotic resistance genes, CRISPR/Cas system has been developed into a new gene-editing tool for the prevention and control of bacterial drug resistance. CRISPR-Cas plays a potentially important role in controlling horizontal gene transfer and limiting the spread of antibiotic resistance. In this review, we will introduce the structure and working mechanism of CRISPR-Cas systems, followed by delivery strategies, and then focus on the relationship between antimicrobial resistance and CRISPR-Cas. Moreover, the challenges and prospects of this research field are discussed, thereby providing a reference for the prevention and control of the spread of antibiotic resistance.

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Crossref · 10.2147/idr.s370869

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  • Some bZIP transcription factors can act as negative regulators under specific conditions, influencing drought stress responses through complex interactions with other signaling pathways.

    “While bZIP transcription factors are generally associated with positive regulation, some bZIP factors can act as negative regulators under specific conditions, influencing drought stress responses through complex interactions with other signaling pathways (Tao et al., 2022).”

    From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Tao et al., 2022

    The paper’s bacterial-biofilms section discusses CRISPR-Cas and antibiotic resistance, not bZIP transcription factors or drought responses. Searches for bZIP negative regulation and plant drought-stress signaling found no passage addressing the claim; the closest results were unrelated references. No page number is available in our copy. (LLM, conf=0.77)

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Authors
Tao, S., Chen, H., Li, N., & Liang, W.
Affiliations
  • Jiangsu University (CN)
  • Bengbu Medical College (CN)
Corresponding author
Liang, Wei
Venue
Infection and Drug Resistance
Published
2022-08
Volume
Volume 15
Pages
4155-4168
ISSN
1178-6973 (ISSN-L)
OpenAlex
W4289260617
Cited by
147
References
150
Citations by year
2026: 38 · 2025: 53 · 2024: 42 · 2023: 13 · 2022: 1
FWCI
2.84
Type
journal article
Language
en
Open access
Locations
  • Infection and Drug Resistance (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Funders
  • Jiangsu Commission of Health (H2018073)
  • Jiangsu University (BRA2019248, H2018073, SF2015, BK20191210)
  • Government of Jiangsu Province (333 Project)
  • Natural Science Foundation of Jiangsu Province (BK20191210, 333 Project)
  • Lianyungang Science and Technology Bureau
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
CRISPR-Cas, antimicrobial resistance, antibiotic resistance genes, horizontal gene transfer, mobile genetic elements, CRISPR gene editing, bacterial adaptive immunity, plasmids, bacteriophages

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