Advances in Candida detection platforms for clinical and point-of-care applications
Open accessAbstract
Invasive candidiasis remains one of the most serious community and healthcare-acquired infections worldwide. Conventional Candida detection methods based on blood and plate culture are time-consuming and require at least 2-4 days to identify various Candida species. Despite considerable advances for candidiasis detection, the development of simple, compact and portable point-of-care diagnostics for rapid and precise testing that automatically performs cell lysis, nucleic acid extraction, purification and detection still remains a challenge. Here, we systematically review most prominent conventional and nonconventional techniques for the detection of various Candida species, including Candida staining, blood culture, serological testing and nucleic acid-based analysis. We also discuss the most advanced lab on a chip devices for candida detection.
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Because it generally takes at least 2-4 days after blood culture collection to detect and identify Candida species, and because access to AFST may be limited or delayed, clinicians usually select initial antifungal treatment based on local species epidemiology, antifungal resistance patterns, and individual patient factors.
“Because it generally takes at least 2–4 days after blood culture collection to detect and identify Candida species, and because access to AFST may be limited or delayed, clinicians usually select initial antifungal treatment based on local species epidemiology, antifungal resistance patterns, and individual patient factors [13–16].”
From Treatment practices for adults with candidemia at 9 active surveillance sites—United States, 2017–2018. (2021) · cites this paper as [16]
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- [87]Medical microbiology 7 Philadelphia, PA Elsevier Saunders 2013
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- Authors
- Safavieh, M., Coarsey, C., Esiobu, N., Memic, A., Vyas, J. M., Shafiee, H., & Asghar, W.
- Affiliations
- Brigham and Women's Hospital (US)
- Florida Atlantic University (US)
- Massachusetts General Hospital (US)
- Corresponding author
- Shafiee, Hadi
- Asghar, Waseem
- ORCID
- Venue
- Critical Reviews in Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2016-04-19
- Volume
- 37
- Issue
- 4
- Pages
- 441-458
- ISSN
- 0738-8551 (ISSN-L)
- 1549-7801
- PMC
- PMC5083221
- OpenAlex
- W2336043212
- Cited by
- 57
- References
- 165
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 4 · 2022: 6 · 2021: 13 · 2020: 3 · 2019: 6 · 2018: 7 · 2017: 4
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Critical Reviews in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (F1F32AI102590,R01AI118502)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- Candida detection, invasive candidiasis, point-of-care testing, lab-on-a-chip, blood culture, serological testing, nucleic acid detection, portable diagnostic device
- MeSH terms
- Point-of-Care Testing, Candida albicans, Candidiasis, Cross Infection, Humans, Point-of-Care Systems, Lab-On-A-Chip Devices
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