CoChat
Sign Up
Cited by 39

The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

Pathological Changes in Alzheimer’s Disease Analyzed Using Induced Pluripotent Stem Cell-Derived Human Microglia-Like Cells

Xu, Mei, Zhang, Lin, Liu, Gang, Jiang, Ning, Zhou, Wenxia, Zhang, Yongxiang·2019·Journal of Alzheimer's Disease
DOI 10.3233/jad-180722
Open this paper in CoChat — free account

Abstract

Microglia constitute the majority of innate immune cells in the brain, and their dysfunction is associated with various central nervous system diseases. Human microglia are extremely difficult to obtain experimentally, thereby limiting studies on their role in complex diseases. Microglia derived from human stem cells provide new tools to assess the pathogenesis of complex diseases and to develop effective treatment methods. This study aimed to develop a reliable method to derive human microglial-like cells (iMGLs) from induced pluripotent stem cells (iPSCs) expressing microglia-specific markers IBA1 and TMEM119 and respond to lipopolysaccharide (LPS) stimulation. Thereafter, we compared iMGL functions from Alzheimer's disease (AD) patients and cognitive normal controls (CNCs). AD-iMGLs displayed stronger phagocytic ability with or without stimulation. High LPS concentrations (>2μg/ml) caused death in CNC-iMGLs, while AD-iMGLs did not display significant cell death. Cytokine analysis revealed that TNF-α, IL-6, and IL-10 secreted by AD-iMGLs were significantly increased upon LPS stimulation compared to those in CNC-iMGLs. The present results indicate that AD-iMGLs exhibit significant inflammatory characteristics and can reflect some pathological changes in microglia in AD, thereby providing new valuable tools to screen candidate drugs for AD and to elucidate the mechanisms underlying AD pathogenesis.

Overview

Integrity signals

Registry

Crossref · 10.3233/jad-180722

Cites

12 of 12 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 40

  1. Ginhoux, 2010, Fate mapping analysis reveals that adult microglia derive from primitive macrophages, Science, 330, 841, 10.1126/science.1194637 · DOI · Paper page
  2. Ransohoff, 2010, The myeloid cells of the central nervous system parenchyma, Nature, 468, 253, 10.1038/nature09615 · DOI · Paper page
  3. Hefendehl, 2014, Homeostatic and injury-induced microglia behavior in the aging brain, Aging Cell, 13, 60, 10.1111/acel.12149 · DOI · Paper page
  4. Davalos, 2005, ATP mediates rapid microglial response to local brain injury in vivo, Nat Neurosci, 8, 752, 10.1038/nn1472 · DOI · Paper page
  5. Azevedo, 2013, Activated microglia mediate synapse loss and short-term memory deficits in a mouse model of transthyretin-related oculoleptomeningeal amyloidosis, Cell Death Dis, 4, e789, 10.1038/cddis.2013.325 · DOI
  6. Orre, 2014, Isolation of glia from Alzheimer’s mice reveals inflammation and dysfunction, Neurobiol Aging, 35, 2746, 10.1016/j.neurobiolaging.2014.06.004 · DOI · Paper page
  7. Condello, 2015, Microglia constitute a barrier that prevents neurotoxic protofibrillar Abeta42 hotspots around plaques, Nat Commun, 6, 6176, 10.1038/ncomms7176 · DOI · Paper page
  8. Salter, 2014, Sublime microglia: Expanding roles for the guardians of the CNS, Cell, 158, 15, 10.1016/j.cell.2014.06.008 · DOI · Paper page
  9. Pandya, 2017, Differentiation of human and murine induced pluripotent stem cells to microglia-like cells, Nat Neurosci, 20, 753, 10.1038/nn.4534 · DOI · Paper page
  10. Muffat, 2016, Efficient derivation of microglia-like cells from human pluripotent stem cells, Nat Med, 22, 1358, 10.1038/nm.4189 · DOI · Paper page
  11. Douvaras, 2017, Directed differentiation of human pluripotent stem cells to microglia, Stem Cell Rep, 8, 1516, 10.1016/j.stemcr.2017.04.023 · DOI · Paper page
  12. Abud, 2017, iPSC-derived human microglia-like cells to study neurological diseases, Neuron, 94, 278, 10.1016/j.neuron.2017.03.042 · DOI · Paper page
  13. Takata, 2017, Induced-pluripotent-stem-cell-derived primitive macrophages provide aplatform for modeling tissue-resident macrophage differentiation and function, Immunity, 47, 183, 10.1016/j.immuni.2017.06.017 · DOI
  14. Brownjohn, 2018, Functional studies of missense TREM2 mutations in human stem cell-derived microglia, Stem Cell Rep, 10, 1294, 10.1016/j.stemcr.2018.03.003 · DOI · Paper page
  15. Amos PJ , Fung S , Case A , Kifelew J , Osnis L , Smith CL , Green K , Naydenov A , Aloi M , Hubbard JJ , Ramakrishnan A , Garden GA , Jayadev S (2017) Modulation of hematopoietic lineage specification impacts TREM2 expression in microglia-like cells derived from human stem cells. ASN Neuro 9, 1759091417716610. · DOI
  16. Scheltens, 2016, Alzheimer’s disease, Lancet, 388, 505, 10.1016/S0140-6736(15)01124-1 · DOI · Paper page
  17. Heneka, 2015, Neuroinflammationin Alzheimer’s disease, Lancet Neurol, 14, 388, 10.1016/S1474-4422(15)70016-5 · DOI · Paper page
  18. Nasica-Labouze, 2015, Amyloid beta protein and Alzheimer’s disease: When computer simulations complement experimental studies, Chem Rev, 115, 3518, 10.1021/cr500638n · DOI
  19. Keren-Shaul, 2017, A unique microglia type associated with restricting development of Alzheimer’s disease, Cell, 169, 1276, 10.1016/j.cell.2017.05.018 · DOI · Paper page
  20. Yang, 2011, Mechanism mediating oligomeric Abeta clearance by naive primary microglia, Neurobiol Dis, 42, 221, 10.1016/j.nbd.2011.01.005 · DOI · Paper page
  21. Spangenberg, 2017, Inflammation in Alzheimer’s disease: Lessons learned from microglia-depletion models, Brain Behav Immun, 61, 1, 10.1016/j.bbi.2016.07.003 · DOI · Paper page
  22. Takahashi, 2006, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell, 126, 663, 10.1016/j.cell.2006.07.024 · DOI · Paper page
  23. Hinze, 2011, Differentiation of mouse bone marrow derived stem cells toward microglia-like cells, BMC Cell Biol, 12, 35, 10.1186/1471-2121-12-35 · DOI
  24. Etemad, 2012, A novel in vitro human microglia model: Characterization of human monocyte-derived microglia, J Neurosci Methods, 209, 79, 10.1016/j.jneumeth.2012.05.025 · DOI
  25. Bennett, 2016, New tools for studying microglia in the mouse and human CNS, Proc Natl Acad Sci USA, 113, E1738, 10.1073/pnas.1525528113 · DOI · Paper page
  26. Singleton, 1996, Insulinlike growth factor I receptor prevents apoptosis and enhances neuroblastoma tumorigenesis, Cancer Res, 56, 4522
  27. Halle, 2008, The NALP3 inflammasome is involved in the innate immune response to amyloid-beta, Nat. Immunol, 9, 857, 10.1038/ni.1636 · DOI · Paper page
  28. Bornemann, 2001, Abeta-induced inflammatory processes in microglia cells of APP23 transgenic mice, Am J Pathol, 158, 63, 10.1016/S0002-9440(10)63945-4 · DOI
  29. 10.1002/cne.24484 · DOI
  30. Lue, 2010, Microglia activation and anti-inflammatory regulation in Alzheimer’s disease, Mol Neurobiol, 41, 115, 10.1007/s12035-010-8106-8 · DOI
  31. Neniskyte, 2013, Lactadherin/MFG-E8 is essential for microglia-mediated neuronal loss and phagoptosis induced by amyloid ß, J Neurochem, 126, 312, 10.1111/jnc.12288 · DOI
  32. Ji, 2007, Resident microglia die and infiltrated neu-trophils and monocytes become major inflammatory cells in lipopolysaccharide-injected brain, Glia, 55, 1577, 10.1002/glia.20571 · DOI
  33. Miwa, 1997, Lipopolysaccharide enhances synthesis of brain-derived neurotrophic factor in cultured rat microglia, J Neurosci Res, 50, 1023, 10.1002/(SICI)1097-4547(19971215)50:6<1023::AID-JNR13>3.0.CO;2-5 · DOI
  34. Elkabes, 1998, Lipopolysaccharide differentially regulates microglial trk receptor and neu-rotrophin expression, J Neurosci Res, 54, 117, 10.1002/(SICI)1097-4547(19981001)54:1<117::AID-JNR12>3.0.CO;2-4 · DOI
  35. Bartlett, 2013, P2X7 receptor activation induces reactive oxygen species formation and cell death in murine EOC13 microglia, Mediators Inflamm, 2013, 271813, 10.1155/2013/271813 · DOI
  36. Qin, 2017, Presenilin 2 deficiency facilitates Aß-induced neuroinflammation and injury by upregulating P2X7 expression, Sci China Life Sci, 60, 189, 10.1007/s11427-016-0347-4 · DOI
  37. 10.1038/s41380-018-0108-3 · DOI · Paper page
  38. Sugiura, 2014, Induced pluripotent stem cell generation-associated point mutations arise during the initial stages of the conversion of these cells, Stem Cell Rep, 2, 52, 10.1016/j.stemcr.2013.11.006 · DOI
  39. Young, 2012, Background mutations in parental cells account for most of the genetic heterogeneity of induced pluripotent stem cells, Cell Stem Cell, 10, 570, 10.1016/j.stem.2012.03.002 · DOI
  40. Mills, 2013, Clonal genetic and hematopoietic heterogeneity among human-induced pluripotent stem cell lines, Blood, 122, 2047, 10.1182/blood-2013-02-484444 · DOI

Cited by

Sorted by citations

Metadata

Authors
Xu, M., Zhang, L., Liu, G., Jiang, N., Zhou, W., & Zhang, Y.
Affiliations
Beijing Institute of Pharmacology and Toxicology, Beijing, P.R. China
Venue
Journal of Alzheimer's Disease
Publisher
SAGE Publications
Published
2019-01-08
Volume
67
Issue
1
Pages
357-368
ISSN
  • 1387-2877
  • 1875-8908
DOI
10.3233/jad-180722
Cited by
39
References
12

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review