Cited by 19Advanced Fluorescence Microscopy Techniquesbioluminescence and chemiluminescence researchGene Regulatory Network Analysis

Imaging Approaches for the Study of Metabolism in Real Time Using Genetically Encoded Reporters

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Chandris, Panagiotis, Giannouli, Christina C., Panayotou, George·2022·Frontiers in Cell and Developmental Biology

Abstract

Metabolism comprises of two axes in order to serve homeostasis: anabolism and catabolism. Both axes are interbranched with the so-called bioenergetics aspect of metabolism. There is a plethora of analytical biochemical methods to monitor metabolites and reactions in lysates, yet there is a rising need to monitor, quantify and elucidate in real time the spatiotemporal orchestration of complex biochemical reactions in living systems and furthermore to analyze the metabolic effect of chemical compounds that are destined for the clinic. The ongoing technological burst in the field of imaging creates opportunities to establish new tools that will allow investigators to monitor dynamics of biochemical reactions and kinetics of metabolites at a resolution that ranges from subcellular organelle to whole system for some key metabolites. This article provides a mini review of available toolkits to achieve this goal but also presents a perspective on the open space that can be exploited to develop novel methodologies that will merge classic biochemistry of metabolism with advanced imaging. In other words, a perspective of “watching metabolism in real time.”

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  • For PdhR-based FRET sensors, increasing pyruvate concentrations result in enhanced CFP emission and reduced YFP emission.

    “Similar to other previously reported PdhR-based FRET sensors74,75, increasing pyruvate concentrations result in enhanced CFP emission and reduced YFP emission.”

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Authors
Chandris, P., Giannouli, C. C., & Panayotou, G.
Affiliations
  • Alexander Fleming Biomedical Sciences Research Center (GR)
  • Academy of Athens (GR)
Corresponding author
Chandris, Panagiotis
Venue
Frontiers in Cell and Developmental Biology
Published
2022-01-18
Volume
9
Pages
725114-725114
ISSN
2296-634X (ISSN-L)
OpenAlex
W4205370446
Cited by
19
References
138
Citations by year
2026: 4 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 1
FWCI
0.58
Type
journal article
Language
en
Open access
Locations
  • Frontiers in Cell and Developmental Biology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
Keywords
genetically encoded reporters, metabolic flux, bioenergetics, anabolism, catabolism, metabolite kinetics, live-cell imaging

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