Imaging Approaches for the Study of Metabolism in Real Time Using Genetically Encoded Reporters
Open accessAbstract
Metabolism comprises of two axes in order to serve homeostasis: anabolism and catabolism. Both axes are interbranched with the so-called bioenergetics aspect of metabolism. There is a plethora of analytical biochemical methods to monitor metabolites and reactions in lysates, yet there is a rising need to monitor, quantify and elucidate in real time the spatiotemporal orchestration of complex biochemical reactions in living systems and furthermore to analyze the metabolic effect of chemical compounds that are destined for the clinic. The ongoing technological burst in the field of imaging creates opportunities to establish new tools that will allow investigators to monitor dynamics of biochemical reactions and kinetics of metabolites at a resolution that ranges from subcellular organelle to whole system for some key metabolites. This article provides a mini review of available toolkits to achieve this goal but also presents a perspective on the open space that can be exploited to develop novel methodologies that will merge classic biochemistry of metabolism with advanced imaging. In other words, a perspective of “watching metabolism in real time.”
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For PdhR-based FRET sensors, increasing pyruvate concentrations result in enhanced CFP emission and reduced YFP emission.
“Similar to other previously reported PdhR-based FRET sensors74,75, increasing pyruvate concentrations result in enhanced CFP emission and reduced YFP emission.”
From Energy paradox in REM sleep: balancing supply and consumption in brain metabolism (2026) · cites this paper as [75]
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Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism
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Genetic architecture of human brain evolution
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“Similar to other previously reported PdhR-based FRET sensors 74,75 , increasing pyruvate concentrationsresultinenhancedCFPemissionandreducedYFPemission.”
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Gene Expression and Editing in Mammalian Retinal Ganglion Cells
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Genetically Encoded Metabolic Sensors to Study Retina Metabolism
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Characterizing spatiotemporal microbial colony distributions in printed PEG-DA hydrogel films
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Mechano-metabolic control of multicellular morphogenesis
Bodineau, C., Bubna-Litic, M., Eicher, A. K., Pourquié, O., & Mongera, A. (2026) · Development · doi:10.1242/dev.204876
- Authors
- Chandris, P., Giannouli, C. C., & Panayotou, G.
- Affiliations
- Alexander Fleming Biomedical Sciences Research Center (GR)
- Academy of Athens (GR)
- Corresponding author
- Chandris, Panagiotis
- Venue
- Frontiers in Cell and Developmental Biology
- Publisher
- Frontiers Media SA
- Published
- 2022-01-18
- Volume
- 9
- Pages
- 725114-725114
- ISSN
- 2296-634X (ISSN-L)
- PMC
- PMC8804523
- OpenAlex
- W4205370446
- Cited by
- 19
- References
- 138
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 1
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Cell and Developmental Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- genetically encoded reporters, metabolic flux, bioenergetics, anabolism, catabolism, metabolite kinetics, live-cell imaging
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