Characterization and Antibacterial Potential of Lactic Acid Bacterium Pediococcus pentosaceus 4I1 Isolated from Freshwater Fish Zacco koreanus
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This study was undertaken to characterize a lactic acid bacterium 4I1, isolated from the freshwater fish, Zacco koreanus. Morphological, biochemical and molecular characterization of 4I1 revealed it to be Pediococcus pentosaceus 4I1. The cell free supernatant (CFS) of P. pentosaceus 4I1 exhibited significant (p<0.05) antibacterial effects (inhibition zone diameters: 16.5–20.4 mm) against tested foodborne pathogenic bacteria with MIC and MBC values of 250-500 and 500-1,000 µg/mL, respectively. Further, antibacterial action of CFS of P. pentosaceus 4I1 against two selected bacteria Staphylococcus aureus KCTC-1621 and Escherichia coli O157:H7 was determined in subsequent assays. The CFS of P. pentosaceus 4I1 revealed its antibacterial action against S. aureus KCTC-1621 and E. coli O157:H7 on membrane integrity as confirmed by a reduction in cell viability, increased potassium ion release (900 and 800 mmol/L), reduced absorption at 260-nm (3.99 and 3.77 OD), and increased relative electrical conductivity (9.9 and 9.7%), respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of the CFS of P. pentosaceus 4I1 resulted in the identification of seven major compounds, which included amino acids, fatty acids and organic acids. SEM-based morphological analysis further confirmed the antibacterial effect of CFS of P. pentosaceus 4I1 against S. aureus KCTC-1621 and E. coli O157:H7. In addition, the CFS of P. Pentosaceus 4I1 displayed potent inhibitory effects on biofilms formation by S. aureus KCTC-1621 and E. coli O157:H7. The study indicates the CFS of P. pentosaceus 4I1 offers an alternative means of controlling foodborne pathogens.
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The release of 260 nm absorbing material (DNA and RNA) due to the CFS of probiotic strains leads to the loss of essential cell electrolytes and cell structure and integrity.
“The release of 260 nm absorbing material (DNA and RNA) due to the CFS of probiotic strains leads to the loss of essential cell electrolytes and cell structure and integrity [8].”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [8]
The Results and Discussion report that the cell-free supernatant of P. pentosaceus 4I1 caused DNA and RNA release from two tested pathogens, alongside loss of membrane integrity and essential electrolytes. The claim reverses the proposed relationship: the Discussion treats membrane damage as the explanation for the release, not the release as the cause of structural damage or electrolyte loss. The findings also concern one strain’s supernatant, not probiotic strains generally. Page numbers were not supplied. (LLM, conf=0.86)
Experimental — accuracy not yet measuredIncreased outbreaks of foodborne diseases and antimicrobial resistance of pathogens against commercial antibiotics demand greater interest and need for natural alternative ways to control foodborne pathogens.
“Increased outbreaks of foodborne diseases in recent years along with antimicrobial resistance of pathogens against commercial antibiotics [8] demand greater interest and need for natural alternative ways to control foodborne pathogens.”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [8]
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“Similar results regarding the loss of 260nm absorbing materials of pathogens due to the antimicrobial compounds or LAB were also reported [8, 31, 32].”
BackgroundResultKalhoro, M. S., Visessanguan, W., Nguyen, L. T., & Anal, A. K. (2019) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.14015 · Cited by 17 · see citing works
- Authors
- Bajpai, V. K., Han, J.-H., Rather, I. A., Park, C., Lim, J., Paek, W. K., Lee, J. S., Yoon, J.-I., & Park, Y.-H.
- Affiliations
- Yeungnam University (KR)
- National Science Museum (KR)
- Corresponding author
- Bajpai, Vivek K.
- Rather, Irfan A.
- Lim, Jeongheui
- Park, Yong-Ha
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2016-12-20
- Volume
- 7
- Pages
- 2037-2037
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC5167689
- OpenAlex
- W2563201186
- Cited by
- 76
- References
- 56
- Citations by year
- 2026: 11 · 2025: 8 · 2024: 8 · 2023: 16 · 2022: 5 · 2021: 12 · 2020: 4 · 2019: 8 · 2018: 3 · 2017: 1
- FWCI
- 5.04
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Foundation (2013M3A9A5047052)
- National Research Foundation of Korea (2013M3A9A5047052)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- Pediococcus pentosaceus, lactic acid bacteria, Zacco koreanus, freshwater fish, antibacterial activity, cell-free supernatant, foodborne pathogens, Staphylococcus aureus, Escherichia coli O157:H7, biofilm inhibition, membrane integrity, organic acids, fatty acids, amino acids
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