Cited by 60Probiotics and Fermented FoodsMeat and Animal Product QualityListeria monocytogenes in Food Safety

Investigation of antimicrobial and antioxidant properties of postbiotics produced by lactobacillus rhamnosus and limosilactobacillus reuteri and their potential application in surface decontamination of red meat

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Jalali, Safura, Mojgani, Naheed, Haghighat, Setareh, Sanjabi, Mohammad Reza, Sarem-Nezhad, Solmaz·2024·LWT

Abstract

This study investigated the antimicrobial properties of postbiotics produced by lactobacillus rhamnosus and limosilactobacillus reuteri in surface decontamination of red meat against pathogenic bacteria ( Escherichia coli and Staphylococcus aureus ). The study evaluated postbiotic activity using agar well diffusion, minimum inhibitory concentration (MIC mg/mL), minimum bactericidal concentration (MBC mg/mL), antioxidant properties, total phenolic content (TPC), and organic acid content. Among 18 meat samples, the mixed postbiotic (L. rhamnosus and L. reuteri , 100 mL/mg) displayed the strongest effect against E. coli , achieving a 5.54 log CFU/g reduction after 48 h with a well diffusion diameter of 23 mm, MIC of 20, and MBC of 25. A similar trend was observed for S. aureus , with the mixed postbiotic showing a reduction of 6.9 log CFU/g after 48 h, MIC of 25, and MBC of 50. Interestingly, the mixed postbiotic also exhibited the highest TPC (250.8 mL/mg). L. reuteri postbiotic displayed the strongest antioxidant activity (52.3 mL/mg) and highest organic acid content (18.9 mL/mg). These results suggest the potential of Lactobacilli -derived postbiotics as natural antimicrobials for controlling surface pathogens on meat products. Additionally, the observed link between TPC and antimicrobial activity warrants further investigation into the potential role of antioxidants in enhancing biopreservation efficacy. • Lactobacillus rhamnosus & reuteri postbiotics (100 mL/mg) reduced surface pathogens of meat. • Mixed postbiotics (100 mL/mg) reduced E. coli by 5.54 log CFU/g (48h). • Mixed postbiotic reduced S. aureus by 6.9 log 5 CFU/g, showed highest antioxidant activity (52.3 mL/mg). • L. reuteri postbiotic had the highest organic acid content, potentially contributing to its antimicrobial effects.

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Crossref · 10.1016/j.lwt.2024.116758

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Authors
Jalali, S., Mojgani, N., Haghighat, S., Sanjabi, M. R., & Sarem-Nezhad, S.
Affiliations
  • Islamic Azad University, Tehran (IR)
  • Razi Vaccine and Serum Research Institute (IR)
  • Iranian Research Organization for Science and Technology (IR)
Corresponding author
Mojgani, Naheed
Venue
LWT
Publisher
Elsevier BV
Published
2024-10
Volume
209
Pages
116758
ISSN
0023-6438 (ISSN-L)
OpenAlex
W4402512291
Cited by
59
References
32
Citations by year
2026: 33 · 2025: 26
FWCI
12.71
Type
journal article
Language
en
Open access
Locations
LWT (publishedVersion, cc-by)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
Keywords
postbiotics, Lactobacillus rhamnosus, Lactobacillus reuteri, red meat, surface decontamination, Escherichia coli, Staphylococcus aureus, antimicrobial activity, antioxidant activity, total phenolic content, organic acids, biopreservation, meat safety

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