Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced
Abstract
This study aimed to isolate bacteriocin-like inhibitory substance (BLIS) producing strains from raw buffalo milk and characterize their probiotic and antimicrobial potential. The BT-11 isolate was identified through 16S rDNA gene sequencing and recA gene amplification as Lactobacillus paraplantarum. The BT-11 strain survived in low pH (2, 3) and bile (0.5, 1, and 2%) for 3 hr of incubation. The strain exhibited adhesion to Caco-2 cells (69.85%), auto-aggregation (89.74%), and hydrophobicity (81.76%). The BLIS produced by BT-11 showed antibacterial activity against indicator bacteria including multidrug-resistant pathogens. Time–kill kinetic analysis revealed the reduction in the growth of Staphylococcus aureus and Salmonella typhimurium (>3 Log CFU/ml) in time-dependent manner. BLIS showed biopreservative effect in meat contaminated with Listeria monocytogenes. The results concluded that L. paraplantarum BT-11 is potential probiotic strain with potential to produce BLISs. Practical applications Raw buffalo milk represents excellent source of unexplored potential probiotics with antimicrobial characteristics. In this study, the Lactobacillus paraplantarum BT-11 was isolated from raw buffalo milk and its probiotic potential and bacteriocin-like inhibitory substance (BLIS) was characterized. The results demonstrated that L. paraplantarum BT-11 is promising potential probiotic. The BLIS produced by L. paraplantarum BT-11 showed antagonistic activity against pathogens including multidrug-resistant foodborne bacteria. BLIS also showed biopreservative potential in meat against Listeria monocytogenes. L. paraplantarum and its antimicrobial compound can be used as bio preservative agent in food safety applications.
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One isolate, L. paraplantarum BT-11, produced a bacteriocin-like inhibitory substance (BLIS), as previously reported by the main author.
“One isolate (L. paraplantarum BT-11) produced bacteriocin-like inhibitory substance (BLIS), which has been already reported by the main author [13].”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [13]
Very limited studies have been reported on the biopreservative potential of probiotics from buffalo milk and their antimicrobial metabolites.
“Very limited studies have been reported on biopreservative potential of the probiotics from buffalo milk and their antimicrobial metabolites [11, 13].”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [13]
LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, lowering cholesterol levels, and immunomodulatory activities.
“The LAB are associated with several health benefits such as improvement of gastrointestinal tract conditions, antibacterial and antifungal activities, antiallergic and antioxidant properties, and lowering cholesterol levels and immunomodulatory activities [12, 13].”
From Antimicrobial Activities and Biopreservation Potential of Lactic Acid Bacteria (LAB) from Raw Buffalo (Bubalus bubalis) Milk (2023) · cites this paper as [13]
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Kalhoro, M. S., Anal, A. K., Kalhoro, D. H., Hussain, T., Murtaza, G., & Mangi, M. H. (2023) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2023/8475995 · Cited by 26 · see citing works
“paraplantarum BT-11) produced bacteriocin-like inhibitory substance (BLIS), which has been already reported by the main author [13].”
BackgroundMethodInfluentialSelf-citation
- Authors
- Kalhoro, M. S., Visessanguan, W., Nguyen, L. T., & Anal, A. K.
- Affiliations
- Asian Institute of Technology (TH)
- National Science and Technology Development Agency (TH)
- Corresponding author
- Anal, Anil Kumar
- Venue
- Journal of Food Processing and Preservation
- Publisher
- Wiley
- Published
- 2019-05-20
- Volume
- 43
- Issue
- 8
- ISSN
- 0145-8892 (ISSN-L)
- 1745-4549
- OpenAlex
- W2944967174
- Cited by
- 17
- References
- 59
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 2
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Food Processing and Preservation (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Animal Diversity and Health Studies
- Keywords
- Lactobacillus paraplantarum, probiotics, bacteriocin-like inhibitory substances, antimicrobial activity, multidrug-resistant bacteria, Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, biopreservation, meat preservation, bile tolerance, acid resistance, cell adhesion, self-aggregation, hydrophobicity, food safety
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