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Cited by 58Probiotics and Fermented FoodsReproductive tract infections researchInfant Nutrition and Health

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A Surface Protein From Lactobacillus plantarum Increases the Adhesion of Lactobacillus Strains to Human Epithelial Cells

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Wang, Guangqiang, Zhang, Minghui, Zhao, Jianxin, Xia, Yongjun, Lai, Phoency F.-H., Ai, Lianzhong·2018·Frontiers in Microbiology
DOI 10.3389/fmicb.2018.02858PMC PMC6261977
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Abstract

Adhesion to epithelial cells is considered important for Lactobacillus to exert probiotic effects. In this study, we found that trypsin treatment decreased the adhesion ability of Lactobacillus plantarum AR326 and AR269, which exhibit good adhesion ability, and surface proteins extracts increased the adhesion of the strains with poor adhesion ability. By SDS-PAGE and mass spectrometry analysis, the main component of the surface proteins was detected and identified as a protein of approximately 37 kDa. It was 100% homologous with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from L. plantarum WCFS1. The adhesion of AR326 and AR269 was decreased significantly by blocking with the anti-GAPDH antibody, and GAPDH restored the adhesion of AR326 and AR269 treated with trypsin. In addition, purified GAPDH significantly increased the adhesion of the strains with poor adhesion ability. These results indicated that GAPDH mediates the adhesion of these highly adhesive lactobacilli to epithelial cells and can be used to improve the adhesion ability of probiotics or other bacteria of interest.

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Metadata

Authors
Wang, G., Zhang, M., Zhao, J., Xia, Y., Lai, P. F.-H., & Ai, L.
Affiliations
  • University of Shanghai for Science and Technology (CN)
  • Jiangnan University (CN)
Corresponding author
Ai, Lianzhong
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2018-11-22
Volume
9
Pages
2858-2858
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2018.02858
PMC
PMC6261977
OpenAlex
W2900721819
Cited by
58
References
1
Citations by year
2026: 7 · 2025: 9 · 2024: 8 · 2023: 4 · 2022: 11 · 2021: 14 · 2020: 5
FWCI
4.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • National Natural Science Foundation of China (31401670, 31471721, 31771956)
  • Shanghai Municipal Education Commission
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
Lactobacillus plantarum, bacterial adhesion, epithelial cells, surface proteins, glyceraldehyde-3-phosphate dehydrogenase, probiotics, trypsinization, adhesion improvement

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