Adhesion and aggregation properties of probiotic and pathogen strains
Abstract
Autoaggregation has been correlated with adhesion, which is known to be a prerequisite for colonization and infection of the gastrointestinal tract by many pathogens. The coaggregation properties of probiotic strains with pathogens as well as their ability to displace pathogens are of importance for therapeutic manipulation of the aberrant intestinal microbiota. Consequently, the ability to aggregate and coaggregate are desirable properties for probiotics in health-promoting foods. Aggregation assays and bacterial adhesion to hydrocarbons (BATH test) demonstrated significant differences in cell surface properties among the tested commercial probiotic strains. Hydrophobicity increased when the cells were heat-inactivated. All probiotic strains tested showed aggregation abilities with the pathogen strains tested, but the results were strain-specific and dependent on time and incubation conditions. Our results indicate that the ability to autoaggregate, together with cell-surface hydrophobicity and coaggregation abilities with pathogen strains can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human or animal use.
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Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli.
“Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli [8, 12–16].”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [13]
Auto-aggregation is associated with enhanced colonization, inhibition of pathogenic infections and immunomodulation of the intestinal mucosa.
“Auto-aggregation is a desired property for probiotics which is associated with enhanced colonization, inhibition of pathogenic infections and immunomodulation of the intestinal mucosa [12, 13, 28, 43, 44].”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [13]
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- Authors
- Collado, M. C., Meriluoto, J., & Salminen, S.
- Affiliations
- Åbo Akademi University (FI)
- University of Turku (FI)
- Venue
- European Food Research and Technology
- Published
- 2007-04-12
- Volume
- 226
- Issue
- 5
- Pages
- 1065-1073
- ISSN
- 0373-0174 (ISSN-L)
- 1438-2377
- 1438-2385
- OpenAlex
- W2062523464
- Cited by
- 603
- References
- 33
- Citations by year
- 2026: 43 · 2025: 44 · 2024: 58 · 2023: 50 · 2022: 56 · 2021: 65 · 2020: 50 · 2019: 41 · 2018: 40 · 2017: 41 · 2016: 12 · 2015: 23 · 2014: 16 · 2013: 15 · 2012: 15
- FWCI
- 11.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Food Research and Technology (publishedVersion)
- Åbo Akademi University Research Portal (submittedVersion)
- Funders
- Generalitat Valenciana
- Turun Yliopisto
- Åbo Akademi
- Biotieteiden ja Ympäristön Tutkimuksen Toimikunta
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- probiotics, self-aggregation, co-aggregation, bacterial adhesion, cell surface hydrophobicity, pathogenic bacteria, probiotic screening, thermal inactivation, intestinal colonization, probiotic foods
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