Auto-aggregation and Co-aggregation ability in bifidobacteria and clostridia
Abstract
A total of 142 human and 88 calf bifidobacteria were isolated and identified; approximately 12 % of all isolated strains exhibited auto-aggregation (Agg) phenotype (Agg+). Properties considered to be predicting for their adhesion to intestine, i.e. auto-aggregation, and hydrophobicity were determined by xylene extraction in 18 human and 8 calf origin bifidobacteria. Co-aggregation of 8 human bifidobacteria with 8 clostridia was also evaluated. Agg varied between 16.3 and 96.4 %, hydrophobicity values ranged from 0 to 82.8 %. The strongest Agg and hydrophobicity were observed in B. bifidum and B. merycicum isolates. However, there were no statistically significant correlations between these two properties. Variability in the percentage of Agg and hydrophobicity was observed after cultivation of bifidobacteria on different carbon sources. All bifidobacteria showed co-aggregation ability with clostridia tested but there were remarkable differences depending on specific combinations of strains. The bifidobacterial strains with the highest ability to co-aggregate with clostridia were B. bifidum I4 and B. longum I10 isolated from infants; these strains gave also high values of Agg. Agg properties together with co-aggregation ability with potential pathogen can be used for preliminary selection of probiotic bacteria.
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Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli.
“Auto-aggregation ability correlates positively with adherence to human epithelial cell lines and the ability to co-aggregate with Listeria monocytogenes, Staphylococcus aureus and pathogenic Escherichia coli [8, 12–16].”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [16]
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- Authors
- Vlková, E., Rada, V., Šmehilová, M., & Killer, J.
- Affiliations
- Czech University of Life Sciences Prague (CZ)
- Corresponding author
- Vlková, E.
- ORCID
- Venue
- Folia Microbiologica
- Published
- 2008-05
- Volume
- 53
- Issue
- 3
- Pages
- 263-269
- ISSN
- 0015-5632 (ISSN-L)
- 1874-9356
- OpenAlex
- W2109603905
- Cited by
- 103
- References
- 32
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 11 · 2023: 13 · 2022: 12 · 2021: 12 · 2020: 4 · 2019: 3 · 2018: 9 · 2017: 5 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 5 · 2012: 3
- FWCI
- 2.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Folia Microbiologica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Bifidobacterium, Clostridia, self-aggregation, co-aggregation, bacterial hydrophobicity, intestinal adhesions, probiotic strain selection, Bifidobacterium bifidum, Bifidobacterium longum, carbon source effects
- MeSH terms
- Animals, Bacterial Adhesion, Bifidobacterium, Carbon, Cattle, Clostridium, Culture Media, Feces, Humans, Intestines, Surface Properties, Hydrophobic and Hydrophilic Interactions
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