CoChat
Sign Up
Cited by 7

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Mutually Dependent Clustering of SynDIG4/PRRT1 and AMPA Receptor Subunits GluA1 and GluA2 in Heterologous Cells and Primary Neurons

Plambeck, Kristopher E., He, Chun-Wei, Navarro, Hector H., Díaz, Elva·2022·Frontiers in Molecular Neuroscience
DOI 10.3389/fnmol.2022.788620PMC PMC9024365
Open this paper in CoChat — free account

Abstract

Regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) at synapses is a predominant mechanism for regulating synaptic strength. We identified the transmembrane protein synapse differentiation-induced gene 1 (SynDIG1; SD1) as an AMPAR interacting protein that regulates excitatory synaptic strength and AMPAR number both in vitro and in vivo. The related protein SynDIG4 (SD4; also known as PRRT1) was identified in several independent proteomic screens in complex with AMPARs, suggesting that it may function as an AMPAR auxiliary factor. Here, we show that the co-expression of SD4 with GluA1 or GluA2 homomeric AMPARs in COS cells leads to a 50 or 33% increase in the mean area of AMPAR puncta, respectively. This effect is accentuated when AMPAR puncta are stratified for co-localization with SD4, resulting in a 100 and 65% increase in GluA1 and GluA2 puncta, respectively. Chimeric proteins expressing only the membrane bound domain of SD4 co-expressed with full-length GluA1 or GluA2 recapitulated the effects of wild-type (WT) SD4. Additionally, the mean puncta area of GluA1 or GluA2 chimeras expressing the membrane and C-terminal domains increased significantly when co-localized with WT SD4. Similarly, the co-expression of GluA1 or GluA2 with SD4 results in a significant increase in the mean area of SD4 puncta co-localized with GluA1 or GluA2, respectively. Last, we observed a significant increase in the co-localization of SD4 with GluA1 after glycine induced long-term potentiation (LTP). The mean size of GluA1 puncta was significantly increased when stratified, indicating that co-localization with SD4 increases synaptic GluA1 cluster size during LTP. These data indicate mutually dependent clustering of SD4 and AMPAR subunits both in COS cells and primary hippocampal neurons, suggesting a mechanism for increased synaptic strength during chemical LTP.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3389/fnmol.2022.788620

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 34

  1. Babaei, 2021, NMDA and AMPA receptors dysregulation in Alzheimer’s disease., Eur. J. Pharmacol., 908, 10.1016/j.ejphar.2021.174310 · DOI
  2. Ben-Yaacov, 2017, Molecular mechanism of AMPA receptor modulation by TARP/stargazin., Neuron, 93, 1126.e4, 10.1016/j.neuron.2017.01.032 · DOI
  3. Díaz, 2010, Regulation of AMPA receptors by transmembrane accessory proteins., Eur. J. Neurosci., 32, 261, 10.1111/j.1460-9568.2010.07357.x · DOI
  4. Freund, 2008, Proline-rich sequence recognition domains (PRD): ligands, function and inhibition., Handb. Exp. Pharmacol., 186, 407, 10.1007/978-3-540-72843-6_17 · DOI
  5. Ge, 2021, GluA1-homomeric AMPA receptor in synaptic plasticity and neurological diseases., Neuropharmacology, 197, 10.1016/j.neuropharm.2021.108708 · DOI
  6. Huganir, 2013, AMPARs and synaptic plasticity: the last 25 years., Neuron, 80, 704, 10.1016/j.neuron.2013.10.025 · DOI · Paper page
  7. Jackson, 2011, The expanding social network of ionotropic glutamate receptors: TARPs and other transmembrane auxiliary subunits., Neuron, 70, 178, 10.1016/j.neuron.2011.04.007 · DOI · Paper page
  8. Jacobi, 2017, Diversity in AMPA receptor complexes in the brain., Curr. Opin. Neurobiol., 45, 32, 10.1016/j.conb.2017.03.001 · DOI · Paper page
  9. Kalashnikova, 2010, SynDIG1: an activity-regulated, AMPA- receptor-interacting transmembrane protein that regulates excitatory synapse development., Neuron, 65, 80, 10.1016/j.neuron.2009.12.021 · DOI
  10. Kay, 2000, The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains., FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol., 14, 231, 10.1096/fasebj.14.2.231 · DOI
  11. Kirk, 2016, Distribution of the SynDIG4/proline-rich transmembrane protein 1 in rat brain., J. Comp. Neurol., 524, 2266, 10.1002/cne.23945 · DOI · Paper page
  12. Ling, 2016, Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3., Sci. Rep., 6, 10.1038/srep24029 · DOI
  13. Lissin, 1998, Activity differentially regulates the surface expression of synaptic AMPA and NMDA glutamate receptors., Proc. Natl. Acad. Sci. U.S.A., 95, 7097, 10.1073/pnas.95.12.7097 · DOI
  14. Lovero, 2013, SynDIG1 promotes excitatory synaptogenesis independent of AMPA receptor trafficking and biophysical regulation., PLoS One, 8, 10.1371/journal.pone.0066171 · DOI
  15. Lu, 2009, Subunit composition of synaptic AMPA receptors revealed by a single-cell genetic approach., Neuron, 62, 254, 10.1016/j.neuron.2009.02.027 · DOI · Paper page
  16. Matt, 2018, SynDIG4/Prrt1 is required for excitatory synapse development and plasticity underlying cognitive function., Cell Rep., 22, 2246, 10.1016/j.celrep.2018.02.026 · DOI · Paper page
  17. Martin, 2021, Interaction and subcellular association of PRRT1/SynDIG4 with AMPA receptors., Front. Synaptic Neurosci., 13, 10.3389/fnsyn.2021.705664 · DOI · Paper page
  18. McAllister, 2007, Dynamic aspects of CNS synapse formation., Annu. Rev. Neurosci., 30, 425, 10.1146/annurev.neuro.29.051605.112830 · DOI · Paper page
  19. Plant, 2006, Transient incorporation of native GluR2-lacking AMPA receptors during hippocampal long-term potentiation., Nat. Neurosci., 9, 602, 10.1038/nn1678 · DOI · Paper page
  20. Sällman Almén, 2012, The dispanins: a novel gene family of ancient origin that contains 14 human members., PLoS One, 7, 10.1371/journal.pone.0031961 · DOI
  21. Sanderson, 2016, Receptor-dependent LTD requires transient synaptic incorporation of Ca2+-permeable AMPARs mediated by AKAP150-anchored PKA and calcineurin., Neuron, 89, 1000, 10.1016/j.neuron.2016.01.043 · DOI · Paper page
  22. Scheiffele, 2003, Cell-cell signaling during synapse formation in the CNS., Annu. Rev. Neurosci., 26, 485, 10.1146/annurev.neuro.26.043002.094940 · DOI
  23. Schwenk, 2012, High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes., Neuron, 74, 621, 10.1016/j.neuron.2012.03.034 · DOI · Paper page
  24. Schwenk, 2009, Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors., Science, 323, 1313, 10.1126/science.1167852 · DOI
  25. Shanks, 2012, Differences in AMPA and kainate receptor interactomes facilitate identification of AMPA receptor auxiliary subunit GSG1L., Cell Rep., 1, 590, 10.1016/j.celrep.2012.05.004 · DOI · Paper page
  26. Tomita, 2005, Stargazin modulates AMPA receptor gating and trafficking by distinct domains., Nature, 435, 1052, 10.1038/nature03624 · DOI · Paper page
  27. Tomita, 2003, Functional studies and distribution define a family of transmembrane AMPA receptor regulatory proteins., J. Cell Biol., 161, 805, 10.1083/jcb.200212116 · DOI
  28. Troyano-Rodriguez, 2019, PRRT1 regulates basal and plasticity-induced AMPA receptor trafficking., Mol. Cell. Neurosci., 98, 155, 10.1016/j.mcn.2019.06.008 · DOI · Paper page
  29. Twomey, 2016, Elucidation of AMPA receptor-stargazin complexes by cryo-electron microscopy., Science, 353, 83, 10.1126/science.aaf8411 · DOI
  30. von Engelhardt, 2010, CKAMP44: a brain-specific protein attenuating short-term synaptic plasticity in the dentate gyrus., Science, 327, 1518, 10.1126/science.1184178 · DOI
  31. Yount, 2010, Palmitoylome profiling reveals s-palmitoylation-dependent antiviral activity of IFITM3., Nat. Chem. Biol., 6, 610, 10.1038/nchembio.405 · DOI
  32. Yu, 2021, Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition., Nature, 594, 448, 10.1038/s41586-021-03540-0 · DOI
  33. Zhang, 2021, Gating and modulation of a hetero-octameric AMPA glutamate receptor., Nature, 594, 454, 10.1038/s41586-021-03613-0 · DOI
  34. Zhao, 2016, Architecture of fully occupied GluA2 AMPA receptor-TARP complex elucidated by Cryo-EM., Nature, 536, 108, 10.1038/nature18961 · DOI

Cited by

Sorted by citations

Metadata

Authors
Plambeck, K. E., He, C.-W., Navarro, H. H., & Díaz, E.
Venue
Frontiers in Molecular Neuroscience
Publisher
Frontiers Media SA
Published
2022-04-08
Volume
15
ISSN
1662-5099
DOI
10.3389/fnmol.2022.788620
PMC
PMC9024365
Cited by
7
References
2
Licence
creativecommons.org/licenses/by/4.0/ (vor)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review