Cited by 74Pharmacological Effects of Natural CompoundsSkin Protection and AgingAgricultural and Biological Research

Glycerolic Licorice Extracts as Active Cosmeceutical Ingredients: Extraction Optimization, Chemical Characterization, and Biological Activity

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Ciganović, Petar, Jakimiuk, Katarzyna, Tomczyk, Michał, Zovko Končić, Marijana·2019·Antioxidants

Abstract

A green ultrasound-assisted extraction (UAE) method using glycerol/water mixtures for extraction of licorice (Glycyrrhiza glabra) bioactive constituents was developed in this study. The response surface method, according to the Box-Behnken design, was employed to optimize the extraction parameters: glycerol concentration (X1), temperature (X2), and the amount of herbal drug used in the production (X3). The responses were content of total phenols (TP), TP extraction efficiency (TPy) and the content of licorice characteristic constituents, glabridin (Gla) and isoliquiritigenin (Iso). Response surface analysis predicted the optimal extraction conditions for maximized amounts of TP, Tpy, Gla, and Iso. The extracts were prepared using the calculated conditions. The analysis of the selected constituents confirmed the validity of the model. Furthermore, biological activity of the extracts was tested. The results demonstrate that UAE using glycerol is a fast and efficient method for preparation of extracts with excellent radical scavenging, Fe2+ chelating and antioxidant activity. Furthermore, the observed notable tyrosinase and elastase inhibitory activity of the extracts, as well as their anti-inflammatory activity, indicate the anti-aging properties of the investigated extracts. The fact that the extracts were prepared using the safe, cosmetically active solvent, glycerol, makes them suitable for direct use in specialized cosmeceutical formulations.

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Crossref · 10.3390/antiox8100445

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  • Functional cosmeceutical ingredients with anti-oxidant activity offer protection against oxidative damage of skin macromolecules associated with effects of free radicals and UV radiation on the skin.

    “They also offer protection against oxidative damage of skin macromolecules associated with the effects of free radicals and UV radiation on the skin [58,75].”
  • G. glabra extract obtained by green ultrasound-assisted extraction with glycerol/water mixtures has good anti-oxidant activity, tyrosinase and elastase inhibitory activity, and anti-inflammatory activity, leading to anti-aging properties.

    “Moreover, Ciganovic et al. highlighted for G. glabra extract, obtained by a green ultrasoundassisted extraction method using glycerol/water mixtures, a good anti-oxidant activity, tyrosinase, and elastase inhibitory activity as well as anti-inflammatory activity, leading to excellent anti-aging properties [58].”

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  1. [9]Glória M.B.A. SWEETENERS|Others Encyclopedia of Food Sciences and Nutrition 2nd ed. Caballero B. Academic Press Oxford, MA, USA 2003 5695 5702

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Authors
Ciganović, P., Jakimiuk, K., Tomczyk, M., & Zovko Končić, M.
Affiliations
  • University of Zagreb (HR)
  • Medical University of Białystok (PL)
  • Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, 10000 Zagreb, Croatia
Venue
Antioxidants
Publisher
MDPI AG
Published
2019-10-01
Volume
8
Issue
10
Pages
445
ISSN
2076-3921 (ISSN-L)
OpenAlex
W2978538299
Cited by
74
References
37
Citations by year
2026: 5 · 2025: 8 · 2024: 10 · 2023: 18 · 2022: 18 · 2021: 10 · 2020: 4 · 2019: 1
FWCI
8.31
Type
journal article
Language
en
Open access
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Croatian Science Foundation (IP-2018-01-6504)
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacological Effects of Natural Compounds
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords
licorice, ultrasound-assisted extraction, cosmeceuticals, glabridin, isoliquiritigenin, total phenolic content, antioxidant activity, free radical scavenging, Fe2+ chelation, tyrosinase inhibition, elastase inhibition, anti-inflammatory activity, anti-aging, green extraction

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