In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains
Open accessAbstract
We isolated several strains of human and animal origin, focusing on the genera Lactobacillus and Bifidobacterium. Samples of cow colostrum, feces and saliva of calves and piglets, and infant feces were acquired, bacterial strains were isolated and identified, and twenty strains of lactobacilli and bifidobacteria were shortlisted for further in vitro analysis of adhesion capacity to human cells, surface hydrophobicity, and auto-aggregation. Adhesion capacity was evaluated using a mixture of Caco-2 and HT-29-MTX cells and hydrophobicity was measured using the microbial adhesion to the hydrocarbon method. Lactobacillus reuteri was the most frequently isolated species, followed by L. casei subsp. paracasei and L. gasseri; all strains were isolated from infant feces. Bifidobacteria were represented by the species B. longum (infant feces), and B. thermophilum and B. pseudolongum (calves, piglets). All twenty strains showed good adhesion capacities to the mixed cell culture (17.7–37.2%), particularly, L. reuteri isolates K7 and K14 (37.2% and 35.5%, respectively). In hydrophobicity and auto-aggregation assays, strain-specific differences irrespective of the origin or taxonomic group were observed. Hydrophobicity values varied considerably (from 6.1% to 87.4%), whereas auto-aggregation ability ranged from 21.7% to 69.7%. No relation was observed between hydrophobicity and adhesion capacity; instead, auto-aggregation was apparently related with adhesion.
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Adhesion to epithelial cells is a desirable property for probiotic potential and results in colonizing the gastrointestinal tract, helping probiotic bacteria in competition and proliferation in the intestine.
“One of the main desirable properties to consider in the search for new strains with probiotic potential is related to adhesion to epithelial cells, which consequently results in colonizing the gastrointestinal tract, helping probiotic bacteria in the competition and proliferation in the intestine [38].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [38]
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- Authors
- Krausova, G., Hyrslova, I., & Hynstova, I.
- Affiliations
- Department of Microbiology and Technology, Dairy Research Institute, Ltd., Ke Dvoru 12a, 160 00 Prague, Czech Republic
- Výzkumný Ústav Mlékárenský (CZ)
- ORCID
- Hyrslova, Ivana
- Venue
- Fermentation
- Publisher
- MDPI AG
- Published
- 2019-12-04
- Volume
- 5
- Issue
- 4
- Pages
- 100
- ISSN
- 2311-5637 (ISSN-L)
- OpenAlex
- W2991759969
- Cited by
- 287
- References
- 41
- Citations by year
- 2026: 48 · 2025: 69 · 2024: 60 · 2023: 38 · 2022: 40 · 2021: 25 · 2020: 5
- FWCI
- 13.51
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Fermentation (publishedVersion, cc-by)
- Funders
- Ministerstvo Zemědělství (MZE-RO1419)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- probiotic strains, Lactobacillus, Bifidobacterium, bacterial adhesion, surface hydrophobicity, self-aggregation, Lactobacillus reuteri, Lactobacillus gasseri, Bifidobacterium longum, Bifidobacterium thermophilum, Bifidobacterium pseudolongum, infant feces, cow colostrum, calves, piglets, Caco-2 cells
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