Cited by 23Neuroscience and Neuropharmacology ResearchReceptor Mechanisms and SignalingNeuroendocrine regulation and behavior

Heterodimer of A2A and oxytocin receptors regulating glutamate release in adult striatal astrocytes

Open access
Amato, Sarah, Averna, Monica, Guidolin, Diego, Pedrazzi, Marco, Pelassa, Simone, Capraro, Michela, Passalacqua, Mario, Bozzo, Matteo +6 more·2022·International Journal of Molecular Sciences

Abstract

BACKGROUND: Roles of astrocytes in the modulatory effects of oxytocin (OT) in central nervous system are increasingly considered. Nevertheless, OT effects on gliotransmitter release have been neglected. METHODS: In purified astrocyte processes from adult rat striatum, we assessed OT receptor (OTR) and adenosine A2A receptor expression by confocal analysis. The effects of receptors activation on glutamate release from the processes were evaluated; A2A-OTR heteromerization was assessed by co-immunoprecipitation and PLA. Structure of the possible heterodimer of A2A and OT receptors was estimated by a bioinformatic approach. RESULTS: Both A2A and OT receptors were expressed on the same astrocyte processes. Evidence for A2A-OTR receptor-receptor interaction was obtained by measuring the release of glutamate: OT inhibited the evoked glutamate release, while activation of A2A receptors, per se ineffective, abolished the OT effect. Biochemical and biophysical evidence for A2A-OTR heterodimers on striatal astrocytes was also obtained. The residues in the transmembrane domains 4 and 5 of both receptors are predicted to be mainly involved in the heteromerization. CONCLUSIONS: When considering effects of OT in striatum, modulation of glutamate release from the astrocyte processes and of glutamatergic synapse functioning, and the interaction with A2A receptors on the astrocyte processes should be taken into consideration.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3390/ijms23042326

Cites

103 of 103 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In the striatum, OXTR activation on astrocytes inhibits glutamate release, and D2R-OXTR heterocomplexes facilitate dopaminergic signaling.

    “In the striatum, OXTR activation on astrocytes inhibits glutamate release (Amato et al., 2022), and D2R-OXTR heterocomplexes facilitate dopaminergic signaling (Hernandez-Mondragon et al., 2023; Pérez de la Mora et al., 2016).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 104 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Amato, S., Averna, M., Guidolin, D., Pedrazzi, M., Pelassa, S., Capraro, M., Passalacqua, M., Bozzo, M., Gatta, E., Anderlini, D., Maura, G., Agnati, L. F., Cervetto, C., & Marcoli, M.
Affiliations
  • University of Genoa (IT)
  • University of Padua (IT)
  • The University of Queensland (AU)
  • University of Modena and Reggio Emilia (IT)
Corresponding author
  • Cervetto, Chiara
  • Marcoli, Manuela
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2022-02-19
Volume
23
Issue
4
Pages
2326
ISSN
1422-0067 (ISSN-L)
OpenAlex
W4214663246
Cited by
23
References
104
Citations by year
2026: 4 · 2025: 2 · 2024: 4 · 2023: 10 · 2022: 3
FWCI
2.10
Type
journal article
Language
en
Open access
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • CINECA IRIS Institutial Research Information System (University of Genoa) (submittedVersion, other-oa)
Funders
  • FFABR (Fondo di finanziamento per le attività base di ricerca; MIUR-Italy) (FFABR- to C.C. and M.M.)
  • Ph.D School of the Department of Experimental Medicine (University of Genova) (contribution 2021 to S.A.)
  • MUR (DIFILAB, Grant/Award Number: RBAP11ETKA-005)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
Keywords
oxytocin receptor, adenosine A2A receptor, receptor heterodimerization, striatal astrocytes, glutamate release, gliotransmitter release, astrocyte processes, oxytocin
MeSH terms
Animals, Astrocytes, Corpus Striatum, Male, Oxytocin, Neostriatum, Rats, Sprague-Dawley, Receptors, Oxytocin, Glutamic Acid, Receptor, Adenosine A2A, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review