Cited by 106Retinal Development and DisordersRetinal Diseases and TreatmentsAdvanced Drug Delivery Systems

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Subretinal Injection Techniques for Retinal Disease: A Review

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Irigoyen, Cristina, Amenabar Alonso, Asier, Sanchez-Molina, Jorge, Rodríguez-Hidalgo, María, Lara-López, Araceli, Ruiz-Ederra, Javier·2022·Journal of Clinical Medicine

DOI 10.3390/jcm11164717PMC PMC9409835

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Abstract

Inherited retinal dystrophies (IRDs) affect an estimated 1 in every 2000 people, this corresponding to nearly 2 million cases worldwide. Currently, 270 genes have been associated with IRDs, most of them altering the function of photoreceptors and retinal pigment epithelium. Gene therapy has been proposed as a potential tool for improving visual function in these patients. Clinical trials in animal models and humans have been successful in various types of IRDs. Recently, voretigene neparvovec (Luxturna®) has been approved by the US Food and Drug Administration for the treatment of biallelic mutations in the RPE65 gene. The current state of the art in gene therapy involves the delivery of various types of viral vectors into the subretinal space to effectively transduce diseased photoreceptors and retinal pigment epithelium. For this, subretinal injection is becoming increasingly popular among researchers and clinicians. To date, several approaches for subretinal injection have been described in the scientific literature, all of them effective in accessing the subretinal space. The growth and development of gene therapy give rise to the need for a standardized procedure for subretinal injection that ensures the efficacy and safety of this new approach to drug delivery. The goal of this review is to offer an insight into the current subretinal injection techniques and understand the key factors in the success of this procedure.

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Authors: Irigoyen, C., Amenabar Alonso, A., Sanchez-Molina, J., Rodríguez-Hidalgo, M., Lara-López, A., & Ruiz-Ederra, J.
Affiliations: University of the Basque Country (ES)
Department of Ophthalmology, Donostia University Hospital (HUD), 20014 Donostia San-Sebastián, Spain
Biogipuzkoa Health Research Institute (ES)
Miramoon Pharma S.L., Avenida de Tolosa, 72, 20018 San Sebastián, Spain
ORCID: Irigoyen, Cristina
Sanchez-Molina, Jorge
Rodríguez-Hidalgo, María
Lara-López, Araceli
Venue: Journal of Clinical Medicine
Publisher: MDPI AG
Published: 2022-08-12
Volume: 11
Issue: 16
Pages: 4717
ISSN: 2077-0383 (ISSN-L)
DOI: 10.3390/jcm11164717
PMC: PMC9409835
OpenAlex: W4291465575
Cited by: 106
References: 88
Citations by year: 2026: 28 · 2025: 45 · 2024: 17 · 2023: 16
FWCI: 2.03
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Journal of Clinical Medicine (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: National Institute of Health Carlos III (PI20/01186, PTQ2019-010412, DIN2020-011302)
European Regional Development Fund (Institute of Health Carlos III/ISCIII) (PI20/01186, PTQ2019-010412, DIN2020-011302)
Retinitis Pigmentosa Patients of Gipuzkoa Foundation (BEGISARE) (PI20/01186, PTQ2019-010412, DIN2020-011302)
Spanish Ministry of Science and Innovation (PI20/01186, PTQ2019-010412, DIN2020-011302)
Topics: Health Sciences › Medicine › Ophthalmology › Retinal Development and Disorders
Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords: subretinal injection, retinal gene therapy, inherited retinal disease, RPE65, voretigene neparvovec, viral vectors, photoreceptors, retinal pigment epithelium

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Subretinal Injection Techniques for Retinal Disease: A Review

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