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Cited by 522Essential Oils and Antimicrobial ActivityAntimicrobial agents and applicationsInsect Pest Control Strategies

The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.

Antibacterial and Antioxidant Activity of Essential Oil Terpenes against Pathogenic and Spoilage-Forming Bacteria and Cell Structure-Activity Relationships Evaluated by SEM Microscopy

Open access
Zengin, Hatice, Baysal, Ayse·2014·Molecules
DOI 10.3390/molecules191117773PMC PMC6272013
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Abstract

The antibacterial activity and antioxidant effect of the compounds α-terpineol, linalool, eucalyptol and α-pinene obtained from essential oils (EOs), against pathogenic and spoilage forming bacteria were determined. The antibacterial activities of these compounds were observed in vitro on four Gram-negative and three Gram-positive strains. S. putrefaciens was the most resistant bacteria to all tested components, with MIC values of 2% or higher, whereas E. coli O157:H7 was the most sensitive strain among the tested bacteria. Eucalyptol extended the lag phase of S. Typhimurium, E. coli O157:H7 and S. aureus at the concentrations of 0.7%, 0.6% and 1%, respectively. In vitro cell growth experiments showed the tested compounds had toxic effects on all bacterial species with different level of potency. Synergistic and additive effects were observed at least one dose pair of combination against S. Typhimurium, E. coli O157:H7 and S. aureus, however antagonistic effects were not found in these combinations. The results of this first study are encouraging for further investigations on mechanisms of antimicrobial activity of these EO components.

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Crossref · 10.3390/molecules191117773

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Figures

Figure 1 Inhibition of cells treated with (A) eucalyptol, S. Typhimurium; (B) linalool, S. Typhimurium; (C) eucalyptol, E. coli O157:H7; (D) linalool, E. coli O157:H7; (E) α-terpineol, S. aureus; (F) eucalyptol, S. aureus; (G) linalool, S. aureus; (H) α-terpineol, S. Typhimurium. (T: α-Terpineol, L: Linalool, E: Eucalyptol).
Figure 1 Inhibition of cells treated with (A) eucalyptol, S. Typhimurium; (B) linalool, S. Typhimurium; (C) eucalyptol, E. coli O157:H7; (D) linalool, E. coli O157:H7; (E) α-terpineol, S. aureus; (F) eucalyptol, S. aureus; (G) linalool, S. aureus; (H) α-terpineol, S. Typhimurium. (T: α-Terpineol, L: Linalool, E: Eucalyptol).CC BY · PubMed Central
Figure 2 Inhibition of E. coli O157:H7 treated with α-terpineol/linalool (T/L) combinations at FIC index value. (A) TM vs. LM, LM/2, LM/4, LM/8; (B) TM/2 vs. LM, LM/2, LM/4, LM/8; (C) TM/4 vs. LM, LM/2, LM/4, LM/8; (D) TM/8 vs. LM, LM/2, LM/4, LM/8 (T: α-Terpineol, L: Linalool, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).
Figure 2 Inhibition of E. coli O157:H7 treated with α-terpineol/linalool (T/L) combinations at FIC index value. (A) TM vs. LM, LM/2, LM/4, LM/8; (B) TM/2 vs. LM, LM/2, LM/4, LM/8; (C) TM/4 vs. LM, LM/2, LM/4, LM/8; (D) TM/8 vs. LM, LM/2, LM/4, LM/8 (T: α-Terpineol, L: Linalool, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).CC BY · PubMed Central
Figure 3 Inhibition of E. coli O157:H7 treated with α-terpineol/eucalyptol (T/E) combination at FIC index values. (A) TM vs. EM, EM/2, EM/4, EM/8; (B) TM/2 vs. EM, EM/2, EM/4, EM/8; (C) TM/4 vs. EM, EM/2, EM/4, EM/8; (D) TM/8 vs. EM, EM/2, EM/4, EM/8 (T: α-Terpineol, E: Eucalyptol, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).
Figure 3 Inhibition of E. coli O157:H7 treated with α-terpineol/eucalyptol (T/E) combination at FIC index values. (A) TM vs. EM, EM/2, EM/4, EM/8; (B) TM/2 vs. EM, EM/2, EM/4, EM/8; (C) TM/4 vs. EM, EM/2, EM/4, EM/8; (D) TM/8 vs. EM, EM/2, EM/4, EM/8 (T: α-Terpineol, E: Eucalyptol, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).CC BY · PubMed Central
Figure 4 Inhibition of E. coli O157:H7 treated with linalool/eucalyptol (L/E) combination at FIC index values. (A) LM vs. EM, EM/2, EM/4, EM/8; (B) LM/2 vs. EM, EM/2, EM/4, EM/8; (C) LM/4 vs. EM, EM/2, EM/4, EM/8; (D) LM/8 vs. EM, EM/2, EM/4, EM/8 (L: Linalool, E: Eucalyptol, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).
Figure 4 Inhibition of E. coli O157:H7 treated with linalool/eucalyptol (L/E) combination at FIC index values. (A) LM vs. EM, EM/2, EM/4, EM/8; (B) LM/2 vs. EM, EM/2, EM/4, EM/8; (C) LM/4 vs. EM, EM/2, EM/4, EM/8; (D) LM/8 vs. EM, EM/2, EM/4, EM/8 (L: Linalool, E: Eucalyptol, M: MIC value, M/2: MIC value/2, M/4: MIC value/4, M/8: MIC value/8).CC BY · PubMed Central
Figure 5 Release of cellular material at 260-nm and 280-nm for E. coli O157:H7 (A); S. Typhimurium (B); and S. aureus (C) cells treated with combinations at FIC values. (T: α-Terpineol, L: Linalool, E: Eucalyptol, add: additive; syn: synergy).
Figure 5 Release of cellular material at 260-nm and 280-nm for E. coli O157:H7 (A); S. Typhimurium (B); and S. aureus (C) cells treated with combinations at FIC values. (T: α-Terpineol, L: Linalool, E: Eucalyptol, add: additive; syn: synergy).CC BY · PubMed Central
Figure 6 SEM micrographs of untreated bacterial cells of (A) E. coli O157:H7; treated bacterial cells of E. coli O157:H7 with (B) eucalyptol; (C) linalool; (D) α-terpineol.
Figure 6 SEM micrographs of untreated bacterial cells of (A) E. coli O157:H7; treated bacterial cells of E. coli O157:H7 with (B) eucalyptol; (C) linalool; (D) α-terpineol.CC BY · PubMed Central
Figure 7 SEM micrographs of untreated bacterial cells of (A) S. aureus; treated bacterial cells of S. aureus with (B) eucalyptol; (C) linalool; (D) α-terpineol.
Figure 7 SEM micrographs of untreated bacterial cells of (A) S. aureus; treated bacterial cells of S. aureus with (B) eucalyptol; (C) linalool; (D) α-terpineol.CC BY · PubMed Central
Figure 8 SEM micrographs of treated E. coli O157:H7 cells with combination of the terpenes at FIC values (with their effect): (A) α-terpineol/linalool; (B) α-terpineol/eucalyptol; (C) linalool/eucalyptol (additive).
Figure 8 SEM micrographs of treated E. coli O157:H7 cells with combination of the terpenes at FIC values (with their effect): (A) α-terpineol/linalool; (B) α-terpineol/eucalyptol; (C) linalool/eucalyptol (additive).CC BY · PubMed Central
Figure 9 SEM micrographs of treated S. aureus cells with combination of the terpenes at FIC values (with their effect): (A) α-terpineol/linalool (additive); (B) α-terpineol/linalool (synergistic); (C) linalool/eucalyptol (additive); (D) α-terpineol /eucalyptol (additive).
Figure 9 SEM micrographs of treated S. aureus cells with combination of the terpenes at FIC values (with their effect): (A) α-terpineol/linalool (additive); (B) α-terpineol/linalool (synergistic); (C) linalool/eucalyptol (additive); (D) α-terpineol /eucalyptol (additive).CC BY · PubMed Central

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  1. Kapros, 2009, Cytotoxicity of tea tree oil in tobacco cells, Allelopath. J., 23, 185

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Metadata

Authors
Zengin, H., & Baysal, A.
Affiliations
  • Izmir Institute of Technology (TR)
  • Department of Food Engineering, Izmir Institute of Technology, Urla, Izmir 35437, Turkey
Venue
Molecules
Publisher
MDPI AG
Published
2014-11-03
Volume
19
Issue
11
Pages
17773-17798
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules191117773
PMC
PMC6272013
OpenAlex
W1966480046
Cited by
519
References
65
Citations by year
2026: 31 · 2025: 54 · 2024: 56 · 2023: 59 · 2022: 50 · 2021: 43 · 2020: 72 · 2019: 55 · 2018: 42 · 2017: 30 · 2016: 19 · 2015: 8
FWCI
27.24
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • IYTE GCRIS Database (Izmir Institute of Technology) (submittedVersion, other-oa)
  • İYTE (İZMİR YÜKSEK TEKNOLOJİ ENSTİTÜSÜ) (submittedVersion, other-oa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords
antibacterial activity, antioxidant activity, α-terpineol, linalool, 1,8-cineole, α-pinene, pathogenic bacteria, synergistic and additive effects, Salmonella typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, minimum inhibitory concentration, bacterial growth inhibition
MeSH terms
Eucalyptol, Acyclic Monoterpenes, Cyclohexane Monoterpenes, Bicyclic Monoterpenes, Anti-Bacterial Agents, Antioxidants, Bacteria, Cyclohexanols, Microscopy, Electron, Scanning, Oils, Volatile, Structure-Activity Relationship, Terpenes, Monoterpenes, Cyclohexenes

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