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Cited by 115Dermatology and Skin DiseasesMedicinal Plants and NeuroprotectionContact Dermatitis and Allergies

Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

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Lee, Yonghyeon, Choi, Hyeon Kyeong, N’deh, Kaudjhis Patrick Ulrich, Choi, Young-Jin, Fan, Meiqi, Kim, Eun-kyung, Chung, Kang-Hyun, An, Jeung Hee·2020·Nutrients
DOI 10.3390/nu12020411PMC PMC7071208
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Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

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  • C. asiatica extract inhibits pro-inflammatory cytokines to suppress dermatitis symptoms and reduces infiltration of immune cells into dermal tissue in in vitro and in vivo models of DNCB-induced atopic dermatitis.

    “The pharmacological effects of C. asiatica on 2,4‐dinitrochlorobenzene (DNCB)‐induced skin inflammation has been tested using in vitro and in vivo models of atopic dermatitis, and the results illustrated strong protective activity of C. asiatica extract whereby it inhibited pro‐inflammatory cytokines to effectively suppress dermatitis symptoms, most significantly the reduced infiltration of immune”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [82]

  • C. asiatica shows significant immunosuppressive action in context of atopic dermatitis, where it suppresses infiltration of immune cells and controls inflammation.

    “C. asiatica shows significant immunosuppressive action in the context of atopic dermatitis, where it suppresses the infiltration of immune cells and controls inflammation.”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [82]

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  1. [1]Biedermann T. Skabytska Y. Kaesler S. Volz T. Regulation of T Cell Immunity in Atopic Dermatitis by Microbes: The Yin and Yang of Cutaneous Inflammation Front. Immunol. 2015 6 353 10.3389/fimmu.2015.00353 26217343 PMC4500098 · DOI
  2. [2]Peng W. Novak N. Pathogenesis of atopic dermatitis Clin. Exp. Allergy 2015 45 566 574 10.1111/cea.12495 25610977 · DOI
  3. [3]Avena-Woods C. Overview of Atopic Dermatitis Am. J. Manag. Care 2017 23 115 123 28978208
  4. [4]Nutten S. Atopic Dermatitis: Global Epidemiology and Risk Factors Ann. Nutr. Metab. 2015 66 8 16 10.1159/000370220 25925336 · DOI
  5. [5]Park J.H. Choi J.Y. Son D.J. Park E.K. Song M.J. Hellström M.J. Hong J.T. Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model Int. J. Mol. Sci. 2017 18 738 10.3390/ijms18040738 PMC5412323 28358324 · DOI · Paper page
  6. [6]Brunner P.M. Guttman-Yassk E. Leung D.Y. The immunology of atopic dermatitis and its reversibility with broad-spectrum and targeted therapies J. Allergy Clin. Immunol. 2017 139 S65 S76 10.1016/j.jaci.2017.01.011 28390479 PMC5405702 · DOI
  7. [7]Wollenberg A. Oranje A. Deleuran M. Simon D. Szalai Z. Kunz B. Svensson A. Barbarot S. von Kobyletzki L. Taieb A. ETFAD/EADV Eczema task force 2015 position paper on diagnosis and treatment of atopic dermatitis in adult and paediatric patients J. Eur. Acad. Dermatol. Venereol. 2016 30 729 747 10.1111/jdv.13599 27004560 · DOI
  8. [8]Paller A.S. Tom W.L. Lebwohl M.G. Blumenthal R.L. Boguniewicz M. Call R.S. Eichenfield L.F. Forsha D.W. Rees W.C. Simpson E.L. Efficacy and Safety of Crisaborole Ointment, a Novel, Nonsteroidal Phosphodiesterase 4 (PDE4) Inhibitor for the Topical Treatment of Atopic Dermatitis (AD) in Children and Adults J. Am. Acad. Derm. 2016 75 494 503 10.1016/j.jaad.2016.05.046 27417017 · DOI
  9. [9]Dawid-Pać R. Medicinal plants used in treatment of inflammatory skin diseases Postepy Derm. Alergol. 2013 30 170 177 10.5114/pdia.2013.35620 PMC3834722 24278070 · DOI
  10. [10]Lokanathan Y. Omar N. Ahmad Puzi N.N. Saim A. Hj Idrus R. Recent Updates in Neuroprotective and Neuroregenerative Potential of Centella asiatica Malays. J. Med. Sci. 2016 23 4 14 27540320 PMC4975583
  11. [11]Hashim P. Sidek H. Helan M.H. Sabery A. Palanisamy U.D. Ilham M. Triterpene Composition and Bioactivities of Centella asiatica Molecules 2011 16 1310 1322 10.3390/molecules16021310 21278681 PMC6259745 · DOI · Paper page
  12. [13]Lee H.N. Shin S.A. Choo G.S. Kim H.J. Park Y.S. Kim B.S. Kim S.K. Cho S.D. Nam J.S. Choi C.S. Anti-inflammatory effect of quercetin and galangin in LPS-stimulated RAW264.7 macrophages and DNCB-induced atopic dermatitis animal models Int. J. Mol. Med. 2018 41 888 898 10.3892/ijmm.2017.3296 29207037 PMC5752163 · DOI
  13. [15]Puttarak P. Panichayupakaranant P. Factors affecting the content of pentacyclic triterpenes in Centella asiatica raw materials Pharm. Biol. 2012 50 1508 1512 10.3109/13880209.2012.685946 22966846 · DOI
  14. [16]George M. Joseph L. Ramaswamy Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica extracts Afr. J. Tradit. Complement. Altern. Med. 2009 6 554 559 10.4314/ajtcam.v6i4.57206 20606777 PMC2816466 · DOI
  15. [17]Ju Ho P. Jun Sung J. Ki Cheon K. Jin Tae H. Anti-inflammatory effect of Centella asiatica phytosome in a mouse model of phthalic anhydride-induced atopic dermatitis Phytomedicine 2018 43 110 119 10.1016/j.phymed.2018.04.013 29747743 · DOI
  16. [18]Adtani P.N. Narasimhan M. Punnoose A.M. Kambalachenu H.R. Antifibrotic effect of Centella asiatica Linn and asiatic acid on arecoline-induced fibrosis in human buccal fibroblasts J. Investig. Clin. Dent. 2017 8 e12208 10.1111/jicd.12208 26840561 · DOI · Paper page
  17. [19]Razali N.N.M. Ng C.T. Fong L.Y. Cardiovascular Protective Effects of Centella asiatica and Its Triterpenes: A Review Planta Med. 2019 85 1203 1215 10.1055/a-1008-6138 31539918 · DOI · Paper page
  18. [20]Hao C. Wu B. Hou Z. Xie Q. Liao T. Wang T. Ma D. Asiatic acid inhibits LPS-induced inflammatory response in human gingival fibroblasts Int. Immunopharmacol. 2017 50 313 318 10.1016/j.intimp.2017.07.005 28738247 · DOI
  19. [21]Lee J.W. Park H.A. Kwon O.K. Jang Y.G. Kim J.Y. Choi B.K. Lee H.J. Lee S. Paik J.H. Oh S.R. Asiatic acid inhibits pulmonary inflammation induced by cigarette smoke Int. Immunopharmacol. 2016 39 208 217 10.1016/j.intimp.2016.07.010 27494684 · DOI
  20. [22]Dong S.H. Liu Y.W. Wei F. Tan H.Z. Han Z.D. Asiatic acid ameliorates pulmonary fibrosis induced by bleomycin (BLM) via suppressing pro-fibrotic and inflammatory signaling pathways Biomed. Pharm. 2017 89 1297 1309 10.1016/j.biopha.2017.03.005 28320097 · DOI
  21. [24]Jia Z. Tang M. Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals Food Chem. 1999 64 555 559 10.1016/S0308-8146(98)00102-2 · DOI
  22. [25]Re R. Pellegrini N. Proteggente A. Pannala A. Yang M. Rice-evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay Free Radic. Biol. Med. 1999 26 1231 1237 10.1016/S0891-5849(98)00315-3 10381194 · DOI
  23. [26]Blois M.S. Antioxidant determinations by the use of a stable free radical Nature 1958 181 1199 1200 10.1038/1811199a0 · DOI
  24. [27]Gunasekaran P. Fan M. Kim E.Y. Shin J.H. Lee J.E. Son E.J. Kim J. Hwang E. Yim M.S. Kim E.H. Amphiphilic Triazine Polymer Derivatives as Antibacterial and Anti-atopic Agents in Mice Model Sci. Rep. 2019 9 15161 10.1038/s41598-019-51561-7 31641232 PMC6805867 · DOI
  25. [28]Gupta A.K. Chow M. Prednicarbate (Dermatop): Profile of a corticosteroid J. Cutan. Med. Surg. 2004 8 244 247 10.1177/120347540400800408 16092003 · DOI
  26. [29]Choi E.J. Iwasa M. Han K.I. Kim W.J. Tang Y. Hwang Y.J. Chae J.R. Han W.C. Shin Y.S. Kim E.K. Heat-Killed Enterococcus faecalis EF-2001 Ameliorates Atopic Dermatitis in a Murine Model Nutreients 2016 8 146 10.3390/nu8030146 PMC4808875 26959058 · DOI
  27. [30]Boniface K. Bernard F.X. Garcia M. Gurney A.L. Lecron J.C. Morel F. IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes J. Immunol. 2005 174 3695 3702 10.4049/jimmunol.174.6.3695 15749908 · DOI · Paper page
  28. [31]Arora R. Kumar R. Agarwal A. Reeta K.H. Gupta Y.K. Comparison of three different extracts of Centella asiatica for anti-amnesic, antioxidant and anticholinergic activities: In vitro and in vivo study Biomed. Pharm. 2018 105 1344 1352 10.1016/j.biopha.2018.05.156 30021372 · DOI · Paper page
  29. [32]Yang H.R. Lee H. Kim J.H. Hong D.H. Rho I.R. Kim G.S. Kim E. Kang C. Therapeutic Effect of Rumex japonicus Houtt on DNCB-Induced Atopic Dermatitis-Like Skin Lesions in Balb/c Mice and Human Keratinocyte HaCaT Cells Nutrients 2019 11 573 10.3390/nu11030573 PMC6470878 30866501 · DOI
  30. [33]Zainola M.K. Abd-Hamid A. Yusof S. Muse R. Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban Food Chem. 2003 81 575 581 10.1016/S0308-8146(02)00498-3 · DOI · Paper page
  31. [34]Shukla A. Rasik A.M. Dhawan B.N. Asiaticoside-induced elevation of antioxidant levels in healing wounds Phytother. Res. 1999 13 50 54 10.1002/(SICI)1099-1573(199902)13:1<50::AID-PTR368>3.0.CO;2-V 10189951 · DOI
  32. [35]Mustafa R.A. Hamid A.A. Mohamed S. Abu Bakar F. Total phenolic compounds, flavonoids and radical scavenging activity of 21 selected tropical plants J. Food Sci. 2010 75 28 35 10.1111/j.1750-3841.2009.01401.x 20492146 · DOI
  33. [36]Schmid-Grendelmeier P. Simon D. Simon H.U. Akdis C.A. Wüthrich B. Epidemiology, clinical features, and immunology of the “intrinsic” (non-IgE-mediated) type of atopic dermatitis (constitutional dermatitis) Allergy 2001 56 841 849 10.1034/j.1398-9995.2001.00144.x 11551248 · DOI
  34. [37]Bieber T. Atopic dermatitis Ann. Derm. 2010 22 125 137 10.5021/ad.2010.22.2.125 PMC2883413 20548901 · DOI
  35. [38]Leung D.Y. Boguniewicz M. Howell M.D. Nomura I. Hamid Q.A. New insights into atopic dermatitis J. Clin. Investig. 2004 113 651 657 10.1172/JCI21060 14991059 PMC351324 · DOI · Paper page
  36. [39]Wood L.C. Elias P.M. Calhoun C. Tsai J.C. Grunfeld C. Feingold K.R. Barrier disruption stimulates interleukin-1 alpha expression and release from a pre-formed pool in murine epidermis J. Invest. Derm. 1996 106 397 403 10.1111/1523-1747.ep12343392 8648167 · DOI
  37. [40]Dokmeci E. Herrick C.A. The immune system and atopic dermatitis Semin. Cutan. Med. Surg. 2008 27 138 143 10.1016/j.sder.2008.04.006 18620135 · DOI
  38. [41]Homey B. Steinhoff M. Ruzicka T. Leung D.Y. Cytokines and chemokines orchestrate atopic skin inflammation J. Allergy Clin. Immunol. 2006 118 178 189 10.1016/j.jaci.2006.03.047 16815153 · DOI
  39. [42]Hong S.H. Ku J.M. Kim H.I. Kim T.Y. Seo H.S. Shin Y.C. Ko S.G. Topical Application of KAJD Attenuates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis Symptoms Through Regulation of IgE and MAPK Pathways in BALB/C Mice and Several Immune Cell Types Front. Pharm. 2019 10 1097 10.3389/fphar.2019.01097 PMC6761305 31607928 · DOI
  40. [43]Liu F.T. Goodarzi H. Chen H.Y. IgE, mast cells, and eosinophils in atopic dermatitis Clin. Rev. Allergy Immunol. 2011 41 298 310 10.1007/s12016-011-8252-4 21249468 · DOI
  41. [44]Lim J.M. Lee B. Min J.H. Kim E.Y. Kim J.H. Hong S. Kim J.J. Sohn Y. Jung H.S. Effect of peiminine on DNCB-induced atopic dermatitis by inhibiting inflammatory cytokine expression in vivo and in vitro Int. Immunopharmacol. 2018 56 135 142 10.1016/j.intimp.2018.01.025 29414643 · DOI
  42. [45]Muñoz F.C. Cervantes M.M. Cervantes-García D. Jiménez M. Ventura-Juárez J. Salinas E. Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat J. Immunol. Res. 2017 2017 6935402 10.1155/2017/6935402 28265582 PMC5318634 · DOI
  43. [46]Suárez-Fariñas M. Dhingra N. Gittler J. Shemer A. Cardinale I. de Guzman Strong C. Krueger J.G. Guttman-Yassky E. Intrinsic atopic dermatitis shows similar TH2 and higher TH17 immune activation compared with extrinsic atopic dermatitis J. Allergy Clin. Immunol. 2013 132 361 370 10.1016/j.jaci.2013.04.046 23777851 PMC3991240 · DOI · Paper page
  44. [47]Weller C.L. Collington S.J. Hartnell A. Conroy D.M. Kaise T. Barker J.E. Wilson M.S. Taylor G.W. Jose P.J. Williams T.J. Chemotactic action of prostaglandin E2 on mouse mast cells acting via the PGE2 receptor 3 Proc. Natl. Acad. Sci. USA 2007 104 11712 11717 10.1073/pnas.0701700104 17606905 PMC1913869 · DOI
  45. [48]Hundley T.R. Prasad A.R. Beaven M.A. Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase J. Immunol. 2001 167 1629 1636 10.4049/jimmunol.167.3.1629 11466386 · DOI
  46. [49]Finn D.F. Walsh J.J. Twenty-first century mast cell stabilizers Br. J. Pharm. 2013 170 23 37 10.1111/bph.12138 PMC3764846 23441583 · DOI
  47. [50]O’Mahony L. Akdis M. Akdis C.A. Regulation of the immune response and inflammation by histamine and histamine receptors J. Allergy Clin. Immunol. 2011 128 1153 1162 10.1016/j.jaci.2011.06.051 21824648 · DOI · Paper page
  48. [51]Wu G.S. The functional interactions between the p53 and MAPK signaling pathways Cancer Biol. 2004 3 156 161 10.4161/cbt.3.2.614 14764989 · DOI
  49. [52]Dillman J.F. McGary K.L. Schlager J.J. An inhibitor of p38 MAP kinase downregulates cytokine release induced by sulfur mustard exposure in human epidermal keratinocytes Toxicol. In Vitro 2004 18 593 599 10.1016/j.tiv.2004.01.009 15251176 · DOI
  50. [53]Ipaktchi K. Mattar A. Niederbichler A.D. Hoesel L.M. Hemmila M.R. Su G.L. Remick D.G. Wang S.C. Arbabi S. Topical p38MAPK inhibition reduces dermal inflammation and epithelial apoptosis in burn wounds Shock 2006 26 201 209 10.1097/01.shk.0000225739.13796.f2 16878030 · DOI
  51. [54]Kaminska B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy--from molecular mechanisms to therapeutic benefits Biochim Biophys. Acta 2005 1754 253 262 10.1016/j.bbapap.2005.08.017 16198162 · DOI · Paper page
  52. [55]Tanigawa N. Hagiwara M. Tada H. Komatsu T. Sugiura S. Kobayashi K. Kato Y. Ishida N. Nishida K. Ninomiya M. Acacetin inhibits expression of E-selectin on endothelial cells through regulation of the MAP kinase signaling pathway and activation of NF-κB Immunopharmacol. Immunotoxical. 2013 35 471 477 10.3109/08923973.2013.811596 23855486 · DOI

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Authors
Lee, Y., Choi, H. K., N’deh, K. P. U., Choi, Y.-J., Fan, M., Kim, E.-K., Chung, K.-H., & An, J. H.
Affiliations
  • Seoul National University of Science and Technology (KR)
  • Konkuk University (KR)
  • Department of Food and Nutrition, KC University, Seoul 07661, Korea
Corresponding author
An, Jeung Hee
ORCID
Venue
Nutrients
Publisher
MDPI AG
Published
2020-02-05
Volume
12
Issue
2
Pages
411
ISSN
2072-6643 (ISSN-L)
DOI
10.3390/nu12020411
PMC
PMC7071208
OpenAlex
W3005411878
Cited by
115
References
0
Citations by year
2026: 13 · 2025: 17 · 2024: 34 · 2023: 25 · 2022: 11 · 2021: 10 · 2020: 4
FWCI
9.12
Type
journal article
Language
en
Open access
Licence
Locations
  • Nutrients (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Agriculture, Food and Rural Affairs (G0190300-01)
Topics
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
Keywords
Centella asiatica, atopic dermatitis, DNCB-induced dermatitis, anti-inflammatory activity, immunomodulation, HaCaT keratinocytes, mast cell infiltration, interleukin-6, TNF-α, cytokine expression, skin inflammation, plant extracts
MeSH terms
Administration, Oral, Administration, Topical, Animals, Anti-Inflammatory Agents, Dermatitis, Atopic, Dinitrochlorobenzene, Disease Models, Animal, Inflammation, Mast Cells, Mice, Inbred BALB C, Plant Extracts, Skin, Triterpenes, Cytokines, Centella, Mice, Immunomodulation

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