GlyNAC (Glycine and N-Acetylcysteine) Supplementation in Mice Increases Length of Life by Correcting Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Abnormalities in Mitophagy and Nutrient Sensing, and Genomic Damage
Open accessAbstract
Determinants of length of life are not well understood, and therefore increasing lifespan is a challenge. Cardinal theories of aging suggest that oxidative stress (OxS) and mitochondrial dysfunction contribute to the aging process, but it is unclear if they could also impact lifespan. Glutathione (GSH), the most abundant intracellular antioxidant, protects cells from OxS and is necessary for maintaining mitochondrial health, but GSH levels decline with aging. Based on published human studies where we found that supplementing glycine and N-acetylcysteine (GlyNAC) improved/corrected GSH deficiency, OxS and mitochondrial dysfunction, we hypothesized that GlyNAC supplementation could increase longevity. We tested our hypothesis by evaluating the effect of supplementing GlyNAC vs. placebo in C57BL/6J mice on (a) length of life; and (b) age-associated GSH deficiency, OxS, mitochondrial dysfunction, abnormal mitophagy and nutrient-sensing, and genomic-damage in the heart, liver and kidneys. Results showed that mice receiving GlyNAC supplementation (1) lived 24% longer than control mice; (2) improved/corrected impaired GSH synthesis, GSH deficiency, OxS, mitochondrial dysfunction, abnormal mitophagy and nutrient-sensing, and genomic-damage. These studies provide proof-of-concept that GlyNAC supplementation can increase lifespan and improve multiple age-associated defects. GlyNAC could be a novel and simple nutritional supplement to improve lifespan and healthspan, and warrants additional investigation.
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Supplementation with glycine and N-acetylcysteine ensures correct GSH balance and extends mice lifespan by 24%.
“Recent data from Kumar et al. showed that supplementation with glycine and N-acetylcysteine ensures the correct GSH balance and extends mice lifespan by 24% [199].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [199]
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- Authors
- Kumar, P., Osahon, O. W., & Sekhar, R. V.
- Affiliations
- Baylor College of Medicine (US)
- Corresponding author
- Sekhar, Rajagopal V.
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2022-03-07
- Volume
- 14
- Issue
- 5
- Pages
- 1114
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC8912885
- OpenAlex
- W4221021054
- Cited by
- 83
- References
- 62
- Citations by year
- 2026: 11 · 2025: 22 · 2024: 17 · 2023: 26 · 2022: 7
- FWCI
- 6.12
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Digital Commons-TMC (Texas Medical Center) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- glycine, N-acetylcysteine, lifespan extension, aging, glutathione deficiency, oxidative stress, mitochondrial dysfunction, mitophagy, nutrient sensing, genomic damage, antioxidant supplementation, healthspan, C57BL/6J mice
- MeSH terms
- Nutrients, Acetylcysteine, Animals, Glutathione, Glycine, Longevity, Mice, Inbred C57BL, Mitochondria, Oxidative Stress, Dietary Supplements, Genomics, Mice, Mitophagy
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