Cited by 84Genetics, Aging, and Longevity in Model OrganismsSulfur Compounds in BiologyMitochondrial Function and Pathology

GlyNAC (Glycine and N-Acetylcysteine) Supplementation in Mice Increases Length of Life by Correcting Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Abnormalities in Mitophagy and Nutrient Sensing, and Genomic Damage

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Kumar, Premranjan, Osahon, Ob W., Sekhar, Rajagopal V.·2022·Nutrients

Abstract

Determinants of length of life are not well understood, and therefore increasing lifespan is a challenge. Cardinal theories of aging suggest that oxidative stress (OxS) and mitochondrial dysfunction contribute to the aging process, but it is unclear if they could also impact lifespan. Glutathione (GSH), the most abundant intracellular antioxidant, protects cells from OxS and is necessary for maintaining mitochondrial health, but GSH levels decline with aging. Based on published human studies where we found that supplementing glycine and N-acetylcysteine (GlyNAC) improved/corrected GSH deficiency, OxS and mitochondrial dysfunction, we hypothesized that GlyNAC supplementation could increase longevity. We tested our hypothesis by evaluating the effect of supplementing GlyNAC vs. placebo in C57BL/6J mice on (a) length of life; and (b) age-associated GSH deficiency, OxS, mitochondrial dysfunction, abnormal mitophagy and nutrient-sensing, and genomic-damage in the heart, liver and kidneys. Results showed that mice receiving GlyNAC supplementation (1) lived 24% longer than control mice; (2) improved/corrected impaired GSH synthesis, GSH deficiency, OxS, mitochondrial dysfunction, abnormal mitophagy and nutrient-sensing, and genomic-damage. These studies provide proof-of-concept that GlyNAC supplementation can increase lifespan and improve multiple age-associated defects. GlyNAC could be a novel and simple nutritional supplement to improve lifespan and healthspan, and warrants additional investigation.

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  • Supplementation with glycine and N-acetylcysteine ensures correct GSH balance and extends mice lifespan by 24%.

    “Recent data from Kumar et al. showed that supplementation with glycine and N-acetylcysteine ensures the correct GSH balance and extends mice lifespan by 24% [199].”

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Authors
Kumar, P., Osahon, O. W., & Sekhar, R. V.
Affiliations
Baylor College of Medicine (US)
Corresponding author
Sekhar, Rajagopal V.
Venue
Nutrients
Publisher
MDPI AG
Published
2022-03-07
Volume
14
Issue
5
Pages
1114
ISSN
2072-6643 (ISSN-L)
OpenAlex
W4221021054
Cited by
83
References
62
Citations by year
2026: 11 · 2025: 22 · 2024: 17 · 2023: 26 · 2022: 7
FWCI
6.12
Type
journal article
Language
en
Open access
Locations
  • Nutrients (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Digital Commons-TMC (Texas Medical Center) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
glycine, N-acetylcysteine, lifespan extension, aging, glutathione deficiency, oxidative stress, mitochondrial dysfunction, mitophagy, nutrient sensing, genomic damage, antioxidant supplementation, healthspan, C57BL/6J mice
MeSH terms
Nutrients, Acetylcysteine, Animals, Glutathione, Glycine, Longevity, Mice, Inbred C57BL, Mitochondria, Oxidative Stress, Dietary Supplements, Genomics, Mice, Mitophagy

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