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Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients
Open accessAbstract
AIM: To investigate Fusobacterium nucleatum (F. nucleatum) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS: The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2(-ΔCT) was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25(th) 3, 75(th) 10 vs 2, 25(th) 1, 75(th) 5) (P < 0.01). CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.
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“Numerous studies, of multiple cohorts, of patients with CRC worldwide have found similar associations.23–27”
From Influence of the Gut Microbiome, Diet, and Environment on Risk of Colorectal Cancer (2020) · cites this paper as [25]
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Metadata
- Authors
- Li, Y.-Y., Ge, Q.-X., Cao, J., Zhou, Y.-J., Du, Y.-L., Shen, B., Wan, Y.-J. Y., & Nie, Y.-Q.
- Affiliations
- Guangzhou First People's Hospital (CN)
- Venue
- World Journal of Gastroenterology
- Publisher
- Baishideng Publishing Group Inc.
- Published
- 2016
- Volume
- 22
- Issue
- 11
- Pages
- 3227
- ISSN
- 1007-9327 (ISSN-L)
- PMC
- PMC4789998
- OpenAlex
- W2330539438
- Cited by
- 175
- References
- 38
- Citations by year
- 2026: 7 · 2025: 16 · 2024: 11 · 2023: 13 · 2022: 20 · 2021: 26 · 2020: 25 · 2019: 23 · 2018: 22 · 2017: 10 · 2016: 2
- FWCI
- 9.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- World Journal of Gastroenterology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Guangzhou First People's Hospital
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
- Keywords
- Fusobacterium nucleatum, colorectal cancer, gut microbiota, tumor invasion, lymph node metastasis, Chinese patients
- MeSH terms
- Adult, Aged, Aged, 80 and over, China, DNA, Bacterial, Female, Fusobacterium Infections, Humans, Male, Middle Aged, Retrospective Studies, Risk Factors, Colorectal Neoplasms, Fusobacterium nucleatum, In Situ Hybridization, Fluorescence, Ribotyping, Asian People, Real-Time Polymerase Chain Reaction
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