Salivary Fusobacterium nucleatum serves as a potential diagnostic biomarker for gastric cancer
Open accessAbstract
BACKGROUND: (Fn) primarily colonized in the oral cavity, has been reported to be involved in the development of gastrointestinal tumor. Until now, little is known about the relationship between salivary Fn and GC. AIM: To determine whether salivary Fn could be a biomarker to diagnose GC and explore the influence of Fn on GC cells. METHODS: The abundance of Fn in saliva was quantified by droplet digital polymerase chain reaction in 120 GC patients, 31 atrophic gastritis (AG) patients, 35 non-AG (NAG) patients, 26 gastric polyp (GP) patients, and 20 normal controls (NC) from Qilu Hospital of Shandong University from January 2019 to December 2020. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of Fn as well as traditional serum tumor markers, including carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 19-9, and CA72-4. Transwell assay and wound-healing assay were conducted to assess the influence of Fn infection on GC cells. The expression of epithelial-mesenchymal transition (EMT) markers was detected using western blot assay. RESULTS: < 0.001). ROC curve analysis showed a favorable capability of Fn (73.33% sensitivity; 82.14% specificity; area under the curve: 0.813) in GC diagnosis, which was superior to that of CEA, CA19-9, CA72-4, ferritin, and sialic acid. The Fn level in saliva of GC patients was increased as the TNM stage increased. GC patients with lymph node metastasis had higher Fn levels than those without metastasis. Both transwell and wound-healing assays indicated that Fn infection promoted the migration and invasion of GC cells. Western blot analysis showed that Fn infection decreased the expression of E-cadherin and increased the expressions of N-cadherin, vimentin, and snail. CONCLUSION: Fn abundance in saliva could be used as a promising biomarker to diagnose GC, and Fn infection could promote GC metastasis by accelerating the EMT process.
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Based on reported target strain abundance in saliva (<10^7 cells/mL for each target species) and average saliva volume, the total amount of postbiotic cells required for efficient coaggregation was estimated to be 10^9 bacterial cells/g of product.
“Based on the reported target strain abundance in saliva (<107 cells/mL saliva for each of the target species) [43–46] and the average saliva volume in the mouth under stimulated conditions [47,48], we estimated that the total amount of postbiotic cells required for efficient coaggregation would be 109 bacterial cells/g of product.”
From Coaggregation of oral pathogens by postbiotic lactobacilli (2025) · cites this paper as [45]
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Coaggregation of oral pathogens by postbiotic lactobacilli
Golletz, P., Jensen, S. D., Collignon, M., Hall, C., Khamas, A. B., Møllebjerg, A., Schlafer, S., Meyer, R. L., & Tykwinska, K. (2025) · Journal of oral microbiology · doi:10.1080/20002297.2025.2508483 · Cited by 10 · see citing works
- Authors
- Chen, W.-D., Zhang, X., Zhang, M.-J., Zhang, Y.-P., Shang, Z.-Q., Xin, Y.-W., & Zhang, Y.
- Affiliations
- Qilu Hospital of Shandong University (CN)
- ORCID
- Venue
- World Journal of Gastroenterology
- Publisher
- Baishideng Publishing Group Inc.
- Published
- 2022-08-14
- Volume
- 28
- Issue
- 30
- Pages
- 4120-4132
- ISSN
- 1007-9327 (ISSN-L)
- PMC
- PMC9403436
- OpenAlex
- W4292451879
- Cited by
- 38
- References
- 37
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 9 · 2023: 10 · 2022: 1
- FWCI
- 7.41
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- World Journal of Gastroenterology (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Helicobacter pylori-related gastroenterology studies
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Gastric Cancer Management and Outcomes
- Keywords
- Fusobacterium nucleatum, gastric cancer, salivary biomarkers, diagnostic biomarkers, epithelial-mesenchymal transition, tumor cell migration and invasion, lymph node metastasis, atrophic gastritis, serum tumor markers, E-cadherin, N-cadherin, vimentin, snails
- MeSH terms
- Carcinoembryonic Antigen, Ferritins, Humans, Prognosis, Stomach Neoplasms, Biomarkers, Tumor, Vimentin, Cadherins, Fusobacterium nucleatum, Adenomatous Polyps, CA-19-9 Antigen, N-Acetylneuraminic Acid
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