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Cited by 124MicroRNA in disease regulationPhagocytosis and Immune RegulationImmune Response and Inflammation

miR-24, miR-30b, and miR-142-3p Regulate Phagocytosis in Myeloid Inflammatory Cells

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Naqvi, Afsar Raza, Fordham, Jezrom B, Nares, Salvador·2015·The Journal of Immunology
DOI 10.4049/jimmunol.1401893PMC PMC4323870
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Abstract

Micro-RNAs (miRNAs) are small noncoding RNAs that regulate various biological pathways. As their role in phagocytosis remains poorly understood, we investigated their impact on phagocytosis in myeloid inflammatory cells. Seven miRNAs (miR-24, -30b, -101, 142-3p, -652-3p, -652-5p, and -1275) that were differentially expressed during monocyte to macrophage (Mφ) and monocyte to dendritic cell (DC) differentiation were screened for their potential role in phagocytosis. Among these, overexpression of miR-24, miR-30b, and miR-142-3p in human monocyte-derived Mφ, DC, monocytes, and PBMCs significantly attenuate phagocytosis of Escherichia coli and Staphylococcus aureus, as well as the secretion of inflammatory mediators, including TNF-α, IL-6, and IL-12p40. miRNA-mediated changes in cytokine profiles were observed at transcriptional and/or posttranscriptional levels and importantly exhibit miRNA-specific impact. To examine the underlying mechanism, we monitored the expression of phagocytosis pathway-associated genes and identified several genes that were altered in Mφ and DC transfected with miR-24, miR-30b, and miR-142-3p mimics. Some of these genes with altered expression also harbor putative miRNA binding sites. We show that miR-142-3p directly regulates protein kinase Cα (PKCα), a key gene involved in phagocytosis. Interestingly, miR-142-3p and PKCα exhibit antagonistic expression during Mφ and DC differentiation. Short interfering RNA-mediated knockdown of PKCα in Mφ leads to reduced bacterial uptake, further highlighting the role of the gene in phagocytosis. Overall, these results demonstrate that miR-24, miR-30b, and miR-142-3p regulate phagocytosis and associated cytokine production in myeloid inflammatory cells through modulation of various genes involved in the pathway.

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  • miR-142-3p and PKCα exhibit antagonistic expression during monocyte differentiation.

    “Interestingly, miR-142-3p and PKCα exhibit antagonistic expression during monocyte differentiation.”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [125]

  • miR-142-3p directly regulates PKCα.

    “Naqvi et al. confirmed that miR-142-3p directly regulates protein kinase Cα (PKCα), a key gene involved in phagocytosis.”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [125]

  • miR-24, miR-30b, and miR-142-3p regulate cytokine production associated with phagocytosis stimulation.

    “The authors also demonstrated that miR-24, miR-30b, and miR-142-3p regulate cytokine production associated with phagocytosis stimulation [125].”

    From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [125]

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Cited by

Sorted by citations

Authors
Naqvi, A. R., Fordham, J. B., & Nares, S.
Affiliations
University of Illinois Chicago (US)
Corresponding author
Nares, Salvador
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2015-02
Volume
194
Issue
4
Pages
1916-1927
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.1401893
PMC
PMC4323870
OpenAlex
W2168590981
Cited by
124
References
3
Citations by year
2026: 4 · 2025: 7 · 2024: 7 · 2023: 11 · 2022: 14 · 2021: 12 · 2020: 11 · 2019: 11 · 2018: 15 · 2017: 11 · 2016: 17 · 2015: 4
FWCI
5.18
Type
journal article
Language
en
Open access
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • University of Illinois at Urbana-Champaign
  • University of Illinois at Chicago
  • National Institute of Dental and Craniofacial Research (DE021052, R01DE021052)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
miR-24, miR-30b, miR-142-3p, monocyte-derived macrophages, dendritic cells, monocyte differentiation, protein kinase C alpha, bacterial internalization, Escherichia coli, Staphylococcus aureus, cytokine production, TNF-α, interleukin-6, IL-12
MeSH terms
Flow Cytometry, Fluorescent Antibody Technique, Humans, Inflammation, Phagocytosis, Transfection, Blotting, Western, Cytokines, Reverse Transcriptase Polymerase Chain Reaction, Myeloid Cells, MicroRNAs

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