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SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling

Osborne, Douglas G, Piotrowski, Joshua T, Dick, Christopher J, Zhang, Jin-San, Billadeau, Daniel D·2015·The Journal of Immunology
DOI 10.4049/jimmunol.1402734PMC PMC4402276
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Abstract

A key component in T cell activation is the endosomal recycling of receptors to the cell surface, thereby allowing continual integration of signaling and Ag recognition. One protein potentially involved in TCR transport is sorting nexin 17 (SNX17). SNX proteins have been found to bind proteins involved in T cell activation, but specifically the role of SNX17 in receptor recycling and T cell activation is unknown. Using immunofluorescence, we find that SNX17 colocalizes with TCR and localizes to the immune synapse in T– conjugates. Significantly, knockdown of the SNX17 resulted in fewer T–APC conjugates, lower CD69, TCR, and LFA-1 surface expression, as well as lower overall TCR recycling compared with control T cells. Lastly, we identified the 4.1/ezrin/radixin/moesin domain of SNX17 as being responsible in the binding and trafficking of TCR and LFA-1 to the cell surface. These data suggest that SNX17 plays a role in the maintenance of normal surface levels of activating receptors and integrins to permit optimum T cell activation at the immune synapse.

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Metadata

Authors
Osborne, D. G., Piotrowski, J. T., Dick, C. J., Zhang, J.-S., & Billadeau, D. D.
Affiliations
Department of Immunology, Mayo Clinic College of Medicine , Rochester, MN 55905
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2015-05-01
Volume
194
Issue
9
Pages
4555-4566
ISSN
  • 0022-1767
  • 1550-6606
DOI
10.4049/jimmunol.1402734
PMC
PMC4402276
Cited by
50
References
3
Language
en
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)

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