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Human soluble IL-6 receptor: its detection and enhanced release by HIV infection
Abstract
By using a fluorescence sandwich ELISA for the quantification of soluble human IL-6R, normal human PBMC were found to be induced to release IL-6R into culture supernatant by stimulation with PHA. Furthermore, certain promonocyte cell lines and human T-cell leukemia virus I (HTLV-I)-positive cell lines produced sIL-6R into culture supernatants constitutively. However, this was not found with HTLV-I negative T cell lines and Burkitt’s B cell line. In addition, generation of supernatant IL-6R of the promonocyte cell line was significantly increased 27-fold after PMA treatment and sevenfold after infection with HIV. The released IL-6R molecules were characterized as an apparent m.w. of 50 to 55 kDa by both size-exclusion HPLC and immunoprecipitation of the soluble protein with IL-6R-specific mAb followed by SDS-PAGE analysis. Furthermore, increased levels of serum IL-6R were detected in blood donors seropositive for HIV. Moreover, the released IL-6R could bind efficiently to purified rIL-6 on solid phase and suppressed the proliferative responses of PBMC. These results suggest that the release of soluble IL-6R might be linked to regulatory functions of immune responses induced by IL-6 stimulation during normal and human retrovirus-infected cell growth and differentiation.
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“In contrast, levels of sIL-6R are in the range of 75 ng/ml [65] and sgp130 levels have been found at around 250-400 ng/ml [32, 66].”
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Metadata
- Authors
- Honda, M., Yamamoto, S., Cheng, M., Yasukawa, K., Suzuki, H., Saito, T., Osugi, Y., Tokunaga, T., & Kishimoto, T.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-04-01
- Volume
- 148
- Issue
- 7
- Pages
- 2175-2180
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1510936315
- Cited by
- 331
- References
- 0
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- closed
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