Cited by 967Wound Healing and TreatmentsLiver physiology and pathologyMacrophage Migration Inhibitory Factor

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

Peripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites

Abstract

Fibrocytes are a distinct population of blood-borne cells that display a unique cell surface phenotype (collagen I+/CD11b+/CD13+/CD34+/CD45RO+/MHC class II+/CD86+) and exhibit potent immunostimulatory activities. Circulating fibrocytes rapidly enter sites of tissue injury, suggesting an important role for these cells in wound repair. However, the regulatory processes that govern the differentiation of blood-borne fibrocytes and the mechanisms that underlie the migration of these cells to wound sites are currently not known. We report herein that ex vivo cultured fibrocytes can differentiate from a CD14+-enriched mononuclear cell population and that this process requires contact with T cells. Furthermore, we demonstrate that TGF-β1 (1–10 ng/ml), an important fibrogenic and growth-regulating cytokine involved in wound healing, increases the differentiation and functional activity of cultured fibrocytes. Because fibrocytes home to sites of tissue injury, we examined the role of chemokine/chemokine receptor interactions in fibrocyte trafficking. We show that secondary lymphoid chemokine, a ligand of the CCR7 chemokine receptor, acts as a potent stimulus for fibrocyte chemotaxis in vitro and for the homing of injected fibrocytes to sites of cutaneous tissue injury in vivo. Finally, we demonstrate that differentiated, cultured fibrocytes express α smooth muscle actin and contract collagen gels in vitro, two characteristic features of wound-healing myofibroblasts. These data provide important insight into the control of fibrocyte differentiation and trafficking during tissue repair and significantly expand their potential role during wound healing.

Overview

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Crossref · 10.4049/jimmunol.166.12.7556

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Items 101–200 of 965

Metadata

Authors
Abe, R., Donnelly, S. C., Peng, T., Bucala, R., & Metz, C. N.
Affiliations
  • Laboratory of Vascular Biology, The Picower Institute for Medical Research , Manhasset, NY 11030
  • Laboratory of Medical Biochemistry, The Picower Institute for Medical Research , Manhasset, NY 11030
Corresponding author
  • Abe, Riichiro
  • Peng, Tina
Venue
The Journal of Immunology
Published
2001-06
Volume
166
Issue
12
Pages
7556-7562
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
OpenAlex
W4248565951
Cited by
967
References
40
Citations by year
2026: 13 · 2025: 15 · 2024: 22 · 2023: 25 · 2022: 28 · 2021: 23 · 2020: 37 · 2019: 30 · 2018: 34 · 2017: 31 · 2016: 43 · 2015: 47 · 2014: 58 · 2013: 59 · 2012: 68
FWCI
27.32
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Immunology and Microbiology › Immunology › Macrophage Migration Inhibitory Factor
Keywords
fibrocytes, fibrocyte differentiation, wound healing, TGF-β1, CCR7, tissue damage, myofibroblasts, alpha-smooth muscle actin, collagen gel contraction
MeSH terms
Animals, Blood Cells, Cell Differentiation, Cell Movement, Cells, Cultured, Collagen, Female, Fibroblasts, Fibrosis, Gels, Humans, Injections, Intravenous, Mice, Inbred BALB C, Stem Cells, Wound Healing, Transforming Growth Factor beta, Lipopolysaccharide Receptors, Receptors, Chemokine, Stem Cell Transplantation, Mice, Transforming Growth Factor beta1

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