TCR Engagement Increases Hypoxia-Inducible Factor-1α Protein Synthesis via Rapamycin-Sensitive Pathway under Hypoxic Conditions in Human Peripheral T Cells
Abstract
Peripheral T cells encounter rapid decrease in oxygen tension because they are activated by Ag recognition and migrate into inflammatory sites or tumors. Activated T cells, therefore, are thought to have such machineries that enable them to adapt to hypoxic conditions and execute immune regulation in situ. We have recently shown that survival of CD3-engaged human peripheral blood T cells is prolonged under hypoxic conditions and hypoxia-inducible factor-1 (HIF-1) and its target gene product adrenomedullin play a critical role for the process. It is also shown that hypoxia alone is not sufficient, but TCR-mediated signal is required for accumulation of HIF-1α in human peripheral T cells. In the present study, we showed that TCR engagement does not influence hypoxia-dependent stabilization but stimulates protein synthesis of HIF-1α, most possibly via PI3K/mammalian target of rapamycin system, and that expression of HIF-1α and its target genes is blocked by treatment with rapamycin. Since some of those gene products, e.g., glucose transporters and phosphoglycerokinase, are considered to be essential for glycolysis and energy production under hypoxic conditions and adequate immune reaction in T cells, this TCR-mediated synthesis of HIF-1α may play a pivotal role in peripheral immune response. Taken together, our results may highlight a novel aspect of downstream signal from Ag recognition by TCR and a unique pharmacological role of rapamycin as well.
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Increased Hif-1α protein synthesis following TCR stimulation is mediated by the PI3 kinase/mTOR pathway.
“This increased Hif-1α protein synthesis is mediated by the PI3 kinase/mTOR pathway [69].”
From Hypoxia and hypoxia-inducible factors as regulators of T cell development, differentiation, and function (2013) · cites this paper as [69]
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- Authors
- Nakamura, H., Makino, Y., Okamoto, K., Poellinger, L., Ohnuma, K., Morimoto, C., & Tanaka, H.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- Karolinska Institutet (SE)
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-06
- Volume
- 174
- Issue
- 12
- Pages
- 7592-7599
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1888121068
- Cited by
- 158
- References
- 40
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 8 · 2023: 8 · 2022: 8 · 2021: 10 · 2020: 13 · 2019: 7 · 2018: 8 · 2017: 12 · 2016: 15 · 2015: 11 · 2014: 5 · 2013: 5 · 2012: 10
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Keywords
- T cell receptor signaling, hypoxia-inducible factor-1α, hypoxia, peripheral T cells, PI3K/mTOR pathway, rapamycin, adrenomedullin, T cell survival, glycolysis, glucose transporters, T cell activation
- MeSH terms
- Antibodies, Monoclonal, Cells, Cultured, Gene Expression Regulation, Humans, Protein Kinases, Receptors, Antigen, T-Cell, Transcription Factors, Transfection, Signal Transduction, Cell Hypoxia, T-Lymphocyte Subsets, CD3 Complex, Jurkat Cells, Phosphatidylinositol 3-Kinases, Sirolimus, Hypoxia-Inducible Factor 1, alpha Subunit, TOR Serine-Threonine Kinases
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