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Cited by 439Immunotherapy and Immune ResponsesImmune Response and InflammationImmune responses and vaccinations

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Altered Innate Immune Functioning of Dendritic Cells in Elderly Humans: A Role of Phosphoinositide 3-Kinase-Signaling Pathway

Agrawal, Anshu, Agrawal, Sudhanshu, Cao, Jia-Ning, Su, Houfen, Osann, Kathryn, Gupta, Sudhir·2007·The Journal of Immunology
DOI 10.4049/jimmunol.178.11.6912
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Abstract

Aging represents a state of paradox where chronic inflammation is associated with declining immune responses. Dendritic cells (DCs) are the major APCs responsible for initiating an immune response. However, DC functions in aging have not been studied in detail. In this study, we have compared the innate immune functions of monocyte-derived myeloid DCs from elderly subjects with DCs from young individuals. We show that although phenotypically comparable, DCs from the aging are functionally different from DCs from the young. In contrast to DCs from the young, DCs from elderly individuals display 1) significantly reduced capacity to phagocytose Ags via macropinocytosis and endocytosis as determined by flow cytometry; 2) impaired capacity to migrate in vitro in response to the chemokines MIP-3β and stromal cell-derived factor-1; and 3) significantly increased LPS and ssRNA-induced secretion of TNF-α and IL-6, as determined by ELISA. Investigations of intracellular signaling revealed reduced phosphorylation of AKT in DCs from the aging, indirectly suggesting decreased activation of the PI3K pathway. Because the PI3K-signaling pathway plays a positive regulatory role in phagocytosis and migration, and also functions as a negative regulator of TLR signaling by inducing activation of p38 MAPK, this may explain the aberrant innate immune functioning of DCs from elderly subjects. Results from real-time PCR and protein expression by flow cytometry demonstrated an increased expression of phosphatase and tensin homolog, a negative regulator of the PI3K-signaling pathway, in DCs from the aging. Increased phosphatase and tensin homolog may thus be responsible for the defect in AKT phosphorylation and, therefore, the altered innate immune response of DCs from elderly humans.

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Metadata

Authors
Agrawal, A., Agrawal, S., Cao, J.-N., Su, H., Osann, K., & Gupta, S.
Affiliations
  • University of California, Irvine (US)
  • Division of Basic and Clinical Immunology, University of California , Irvine, CA 92697
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2007-06-01
Volume
178
Issue
11
Pages
6912-6922
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.178.11.6912
OpenAlex
W1508177354
Cited by
435
References
64
Citations by year
2026: 10 · 2025: 20 · 2024: 20 · 2023: 18 · 2022: 20 · 2021: 20 · 2020: 33 · 2019: 28 · 2018: 29 · 2017: 26 · 2016: 22 · 2015: 22 · 2014: 28 · 2013: 34 · 2012: 21
FWCI
8.05
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of California, Irvine
  • National Institute on Aging (AG-027512, R21AG027512)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
Keywords
dendritic cells, aging, innate immunity, phosphoinositide 3-kinase signaling, Akt phosphorylation, phagocytosis, dendritic cell migration, chemokines, CCL19, CXCL12, TNF-α, interleukin-6, lipopolysaccharide, phosphatase and tensin homolog, chronic inflammation, p38 MAPK, Toll-like receptor signaling
MeSH terms
Phosphoinositide-3 Kinase Inhibitors, Adult, Aged, Aged, 80 and over, Aging, Cell Count, Cell Differentiation, Cells, Cultured, Dendritic Cells, Humans, Immunity, Innate, Monocytes, Signal Transduction, Up-Regulation, Cytokines, Inflammation Mediators, Jurkat Cells, Phosphatidylinositol 3-Kinases, PTEN Phosphohydrolase

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