Cited by 149Malaria Research and ControlMolecular Biology Techniques and ApplicationsBiosensors and Analytical Detection

The DOI matches its Crossref record.

A Simple Method to Detect Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Barker, Robert H., Banchongaksorn, Trairat, Courval, Jeanne M., Suwonkerd, Wannapa, Rimwungtragoon, Kamolwan, Wirth, Dyann F.·1992·The American Journal of Tropical Medicine and Hygiene

DOI 10.4269/ajtmh.1992.46.416

Open this paper in CoChat — free account

Abstract

We have developed a simple method for treating blood samples permitting direct detection of Plasmodium falciparum parasites using the P. falciparum-specific DNA probe pPF14 after polymerase chain reaction (PCR) amplification of target DNA sequences, and have compared this method with microscopic examination of thick blood smears. For PCR amplification, blood samples were lysed, then filtered onto filter paper. After drying, a piece of the filter paper was added directly to the PCR mixture for amplification. The presence of PCR products was detected using nonisotopically labeled probe. This method permits detection of less than than 10 parasites in a 20-microliters sample, and minimizes the effects of PCR inhibitors generally found in blood. Samples were collected from patients presenting at malaria clinics in Mae Sod and Mae Ramat, Thailand, and 626 were analyzed both by the PCR method and by conventional microscopy. Of these, 157 were positive both by microscopy and by PCR, while 297 were negative by both methods. PCR detected 131 samples that were negative by microscopy, and failed to detect 41 samples identified as positive by microscopy. All discordant samples were re-analyzed by microscopy and by PCR. Upon re-examination at a higher sensitivity, microscopy identified five additional positive cases, while six previously positive cases were found to be negative. This method of treating blood samples for PCR may also be useful in other diagnostic assays.

Integrity signals

Registry

Crossref · 10.4269/ajtmh.1992.46.416

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Sorted by citations

High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction
Snounou, G., Viriyakosol, S., Zhu, X. P., Jarra, W., Pinheiro, L., do Rosario, V. E., Thaithong, S., & Brown, K. N. (1993) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(93)90077-b · Cited by 1,591
Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections
Snounou, G., Viriyakosol, S., Jarra, W., Thaithong, S., & Brown, K. N. (1993) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(93)90050-8 · Cited by 911
Submicroscopic Infection in Plasmodium falciparum –Endemic Populations: A Systematic Review and Meta‐Analysis
Okell, L. C., Ghani, A. C., Lyons, E., & Drakeley, C. J. (2009) · The Journal of Infectious Diseases · doi:10.1086/644781 · Cited by 532
ACP Broadsheet no 148. July 1996. Laboratory diagnosis of malaria.
Warhurst, D. C., & Williams, J. E. O. (1996) · Journal of Clinical Pathology · doi:10.1136/jcp.49.7.533 · Cited by 331
Detection of Theileria annulata in blood samples of carrier cattle by PCR
d’Oliveira, C., van der Weide, M., Habela, M., Jacquiet, P., & Jongejan, F. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.10.2665-2669.1995 · Cited by 294
“Samples were processed as previously described (2).”
Nested PCR Analysis of Plasmodium Parasites
Snounou, G., & Singh, B. B. (2003) · Humana Press eBooks · doi:10.1385/1-59259-271-6:189 · Cited by 285
PCR and strain identification in Plasmodium falciparum
Wooden, J. M., Kyes, S., & Sibley, C. H. (1993) · Parasitology Today · doi:10.1016/0169-4758(93)90131-x · Cited by 231
Age-Dependent Carriage of Multiple Plasmodium falciparum Merozoite Surface Antigen-2 Alleles in Asymptomatic Malaria Infections
Ntoumi, F., Contamin, H., Rogier, C., Bonnefoy, S., Trape, J., & Mercereau‐Puijalon, O. (1995) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1995.52.81 · Cited by 225
Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces.
Pontes, L. A., Dias‐Neto, E., & Rabello, A. (2002) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2002.66.157 · Cited by 219
Microfluidic approaches to malaria detection
Gascoyne, P. R. C., Satayavivad, J., & Ruchirawat, M. (2003) · Acta Tropica · doi:10.1016/j.actatropica.2003.11.009 · Cited by 206
“First, sample preparation needs to be carefully considered (Lantz et al., 2000; von Wintzingerode et al., 1997; Barker et al., 1992, 1994).”
Background
The importance of sensitive detection of malaria parasites in the human and insect hosts in epidemiological studies, as shown by the analysis of field samples from Guinea Bissau
Snounou, G., Pinheiro, L., Gonçalves, A. C., Fonseca, L. F., Dias, F., Brown, K. N., & do Rosário, V. E. (1993) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/0035-9203(93)90274-t · Cited by 177
Impact of Microscopy Error on Estimates of Protective Efficacy in Malaria‐Prevention Trials
Ohrt, C., Purnomo, P., Sutamihardja, M. A., Tang, D. B., & Kain, K. C. (2002) · The Journal of Infectious Diseases · doi:10.1086/341938 · Cited by 173
“Studies in Thailand and Kenya compared expert microscopy with repeat-smear examination and PCR [7, 8].”
Result
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand
Coleman, R. E., Sattabongkot, J., Promstaporm, S., Maneechai, N., Tippayachai, B., Kengluecha, A., Rachapaew, N., Zollner, G. E., Miller, R. S., Vaughan, J. A., et al. (2006) · Malaria Journal · doi:10.1186/1475-2875-5-121 · Cited by 174
Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR
Fu, Z., Rogelj, S., & Kieft, T. L. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.07.013 · Cited by 166
DNA probes and PCR for diagnosis of parasitic infections
Weiss, J. B. (1995) · Clinical Microbiology Reviews · doi:10.1128/cmr.8.1.113 · Cited by 166
“(8) designed degenerate primers based on the consensus sequence of eight 21-bp repeats.”
Background
Genotyping of Plasmodium falciparum isolates by the polymerase chain reaction and potential uses in epidemiological studies.
Viriyakosol, S., Siripoon, N., Petcharapirat, C., Petcharapirat, P., Jarra, W., Thaithong, S., Brown, K. N., & Snounou, G. (1995) · Europe PMC (PubMed Central) · Cited by 156
PCR typing of field isolates of Plasmodium falciparum
Contamin, H., Fandeur, T., Bonnefoy, S., Skouri, F., Ntoumi, F., & Mercereau‐Puijalon, O. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.4.944-951.1995 · Cited by 133
“To date, PCR has been successfully used as a highly sensitive diagnostic method (3, 28, 31, 32, 36, 38, 40, 41) and for investigating the genetic diversity of specific loci coding for major vaccine candidates such as the MSA-1 (15, 22, 34, 37), MSA-2 (18, 19, 22, 34), or CS (1, 17) protein or for the highly polymorphi…”
Method
Random distribution of mixed species malaria infections in Papua New Guinea.
Mehlotra, R. K., Lorry, K., Kastens, W., et al. (2000) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2000.62.225 · Cited by 128
A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR
Sritharan, V., & Barker, R. H. (1991) · Molecular and Cellular Probes · doi:10.1016/s0890-8508(06)80011-3 · Cited by 127
Self-citation
Detection of Streptococcus pneumoniae in whole blood by PCR
Zhang, Y., Isaacman, D. J., Wadowsky, R. M., et al. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.3.596-601.1995 · Cited by 120
“12533–12537 (5) NA Resistant TCH 12540–12547 (8) NA Resistant TCH 14070–14082 (13) NA Sensitive CHP 14711, 14712 (2) 1 NA CHP 14713, 14714 (2) 3 NA CHP 14715, 14716 (2) 4 NA CHP 14717, (1) 5 NA CHP 14718, 14719 (2) 6B NA CHP 14720, 14721 (2) 7F NA CHP 14722, 14723 (2) 9V NA CHP 14724 (1) 10A NA CHP 14725, 14726 (2) 11…”
Background
Detection of Plasmodium falciparum by Polymerase Chain Reaction in a Field Study
Sethabutr, O., Brown, A. E., Panyim, S., Kain, K. C., Webster, H. K., & Echeverria, P. D. (1992) · The Journal of Infectious Diseases · doi:10.1093/infdis/166.1.145 · Cited by 117
Detection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers
Singh, B. B., Cox‐Singh, J., Miller, A. O., Abdullah, M. S., Snounou, G., & Rahman, H. A. (1996) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/s0035-9203(96)90302-8 · Cited by 112
One year's experience with the polymerase chain reaction as a routine method for the diagnosis of imported malaria.
Morassin, B., Fabre, R., Berry, A., & Magnaval, J. F. (2002) · The American journal of tropical medicine and hygiene · doi:10.4269/ajtmh.2002.66.503 · Cited by 109
Advances in malaria diagnosis
Hawkes, M., & Kain, K. C. (2007) · Expert Review of Anti-infective Therapy · doi:10.1586/14787210.5.3.485 · Cited by 115
Molecular methods for diagnosis and epidemiological studies of parasitic infections
Singh, B. B. (1997) · International Journal for Parasitology · doi:10.1016/s0020-7519(97)00111-2 · Cited by 107
Molecular malaria diagnostics: A systematic review and meta-analysis
Roth, J., Korevaar, D. A., Leeflang, M., & Mens, P. F. (2015) · Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408363.2015.1084991 · Cited by 100
Detection of Theileria annulata in cattle and vector ticks by PCR using the Tams1 gene sequences
Kirvar, E., ILHAN, T., Katzer, F., et al. (2000) · Parasitology · doi:10.1017/s0031182099005466 · Cited by 100
“The DNA from blood samples taken during a field survey of cattle in western Turkey was extracted by the saponin lysis method (Barker et al. 1992).”
Method
Red Blood Cell Polymorphism and Susceptibility to Plasmodium vivax
Zimmerman, P. A., Ferreira, M. U., Howes, R. E., & Mercereau‐Puijalon, O. (2013) · Advances in Parasitology/Advances in parasitology · doi:10.1016/b978-0-12-407826-0.00002-3 · Cited by 104
A colorimetric in vitro drug sensitivity assay for Plasmodium falciparum based on a highly sensitive double-site lactate dehydrogenase antigen-capture enzyme-linked immunosorbent assay.
Druilhe, P., Sabater, A. M., Blanc, C., Brasseur, P., & Jacquier, P. (2001) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2001.64.233 · Cited by 97
Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle
Majiwa, P. A. O., Thatthi, R., Moloo, S. K., et al. (1994) · Parasitology · doi:10.1017/s0031182000076150 · Cited by 91
High rate of mixed and subpatent malarial infections in southwest Nigeria.
May, J., Mockenhaupt, F. P., Ademowo, O. G., Falusi, A. G., Olumese, P. E., Bienzle, U., & Meyer, C. G. (1999) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1999.61.339 · Cited by 84
Identification of bovine Neospora parasites by PCR amplification and specific small-subunit rRNA sequence probe hybridization
Ho, M. S., Barr, B. C., Marsh, A. E., et al. (1996) · Journal of Clinical Microbiology · doi:10.1128/jcm.34.5.1203-1208.1996 · Cited by 82
“PCR has been employed successfully for the detection of hemoprotozoal parasites (4, 5, 14).”
Method
Diagnosing malaria in pregnancy: An update
Fried, M., Muehlenbachs, A., & Duffy, P. E. (2012) · Expert Review of Anti-infective Therapy · doi:10.1586/eri.12.98 · Cited by 80
Low sensitivity of nested PCR using Plasmodium DNA extracted from stained thick blood smears: an epidemiological retrospective study among subjects with low parasitaemia in an endemic area of the Brazilian Amazon region.
Scopel, K. K. G., Fontes, C. J. F., Nunes, Á. C., Horta, M. F., & Braga, É. M. (2004) · Malaria Journal · doi:10.1186/1475-2875-3-8 · Cited by 80
“It has been observed that intrinsic (as DNA amount or a high content of human DNA or haemoglobin) and extrinsic (use of heparin or inadequate conditions of blood collecting, storage and amplification of samples) factors can inhibit the PCR assay [3,18,19].”
Background
Only Viable Parasites Are Detected by PCR following Clearance of Rodent Malarial Infections by Drug Treatment or Immune Responses
Jarra, W., & Snounou, G. (1998) · Infection and Immunity · doi:10.1128/iai.66.8.3783-3787.1998 · Cited by 71
“PCR assays are more sensitive than microscopy and are therefore often used to detect the parasite and identify the species (3, 17, 18, 29, 32, 33).”
Background
Old and Recent Advances in Life Cycle, Pathogenesis, Diagnosis, Prevention, and Treatment of Malaria Including Perspectives in Ethiopia
Nureye, D., & Assefa, S. (2020) · The Scientific World JOURNAL · doi:10.1155/2020/1295381 · Cited by 69
Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon
Morlais, I., Grébaut, P., Bodo, J.-M., et al. (1998) · Acta Tropica · doi:10.1016/s0001-706x(98)00014-x · Cited by 68
“This method has been developed to facilitate the detection of many types of parasite (Meredith et al., 1991; Barker et al., 1992; De Bruijn et al., 1993; Laserson et al., 1994), including trypanosomes (Moser et al.”
Method
Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction
Cox‐Singh, J., Mahayet, S., Abdullah, M. S., et al. (1997) · International Journal for Parasitology · doi:10.1016/s0020-7519(97)00147-1 · Cited by 68
A single-step, PCR-based method for the detection and differentiation of Plasmodium vivax and P. falciparum
Patsoula, E., Spanakos, G., Sofianatou, D., Parara, M., & Vakalis, N. C. (2003) · Annals of Tropical Medicine & Parasitology · doi:10.1179/000349803125002535 · Cited by 65
“The classic diag- Barker et al., 1992; Brown et al., 1992). nostic procedure remains the examination Initially, the target was the parasite’s repetitive of Giemsa-stained bloodsmears.”
BackgroundMethod
Plasmodium falciparum multiple infections in Mozambique, its relation to other malariological indices and to prospective risk of malaria morbidity
Mayor, A. G., Saúte, F., Aponte, J. J., Almeda, J., Gómez-Olivé, F. X., Dgedge, M., & Alonso, P. L. (2003) · Tropical Medicine & International Health · doi:10.1046/j.1365-3156.2003.00968.x · Cited by 64
Species-specific PCR detection of malaria parasites by microtiter plate hybridization: clinical study with malaria patients
Kimura, M., Miyake, H., Kim, H. S., et al. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.9.2342-2346.1995 · Cited by 61
“The sequences of DNA that are amplified and detected are those of the 18S rRNA gene (9, 13, 15), the probes pBRK1-14 (K1-14) (14, 17) and pPF14 containing the 21-bp repeats (3), the ringinfected erythrocyte surface antigen and dihydrofolate reductase-thymidylate synthase genes (8), and the circumsporozoite protein gen…”
BackgroundMethod
Screening donor blood for malaria by polymerase chain reaction
Hang, V. T. T., Be, T. V., Tran, P. N., Thanh, L. T., Hien, L. V., O'Brien, E., & Morris, G. E. (1995) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/0035-9203(95)90652-5 · Cited by 58
Development of a Plasmodium PCR for Monitoring Efficacy of Antimalarial Treatment
Ciceron, L., Jaureguiberry, G., Gay, F., & Danis, M. (1999) · Journal of Clinical Microbiology · doi:10.1128/jcm.37.1.35-38.1999 · Cited by 59
“The first studies of nucleic acidbased malaria detection used the repetitive genomic DNA as a target (3, 22).”
Background
Diagnosis of Mycobacterium avium bacteremia by polymerase chain reaction
Iralu, J. V., Sritharan, V., Pieciak, W., Wirth, D. F., Maguire, J. H., & Barker, R. H. (1993) · Journal of Clinical Microbiology · doi:10.1128/jcm.31.7.1811-1814.1993 · Cited by 57
Self-citation
Malaria diagnosis: standardization of a polymerase chain reaction for the detection of Plasmodium falciparum parasites in individuals with low-grade parasitemia
Zalis, M. G., Ferreira‐da‐Cruz, M. F., Borges, H. C. B. G., Banic, D. M., Alecrim, W. D., de Souza, J. M., Druilhe, P., & Daniel‐Ribeiro, C. T. (1996) · Parasitology Research · doi:10.1007/s004360050173 · Cited by 54
Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification
Aonuma, H., Suzuki, M., Iseki, H., Perera, N., Nelson, B., Igarashi, I., Yagi, T., Kanuka, H., & Fukumoto, S. (2008) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2008.09.061 · Cited by 56
MALARIA
Zucker, J. R., & Campbell, C. C. (1993) · Infectious Disease Clinics of North America · doi:10.1016/s0891-5520(20)30542-0 · Cited by 51
Active case surveillance, passive case surveillance and asymptomatic malaria parasite screening illustrate different age distribution, spatial clustering and seasonality in western Kenya
Zhou, G., Afrane, Y. A., Malla, S., Githeko, A. K., & Yan, G. (2015) · Malaria Journal · doi:10.1186/s12936-015-0551-4 · Cited by 49
Detection ofTrypanosoma brucei gambiense, in serologically positive but aparasitaemic sleeping-sickness suspects in Cameroon, by PCR
Kanmogne, G. D., Asonganyi, T., & Gibson, W. C. (1996) · Annals of Tropical Medicine and Parasitology · doi:10.1080/00034983.1996.11813072 · Cited by 44
STORAGE DURATION AND POLYMERASE CHAIN REACTION DETECTION OF PLASMODIUM FALCIPARUM FROM BLOOD SPOTS ON FILTER PAPER
Chaorattanakawee, S., Natalang, O., Hananantachai, H., et al. (2003) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2003.69.42 · Cited by 42
Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium subpatent infections from the Amazon region in Brazil
Miguel, R. B., Coura, J. R., Samudio, F. E., et al. (2013) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s0036-46652013000300012 · Cited by 41
“(3) Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fiocruz, Pavilhão Artur Neiva, Sala 13.”
BackgroundInfluential
A field study on malaria prevalence in southeastern Laos by polymerase chain reaction assay.
Toma, H., Kobayashi, J., Vannachone, B., Arakawa, T., Sato, Y., Nambanya, S., Manivong, K., & Inthakone, S. (2001) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2001.64.257 · Cited by 41
Informed decision-making before changing to RDT: a comparison of microscopy, rapid diagnostic test and molecular techniques for the diagnosis and identification of malaria parasites in Kassala, eastern Sudan
Osman, M. M. M., Nour, B. Y. M., Sedig, M. F., De Bes, L., Babikir, A. M., Mohamedani, A. A., & Mens, P. F. (2010) · Tropical Medicine & International Health · doi:10.1111/j.1365-3156.2010.02659.x · Cited by 40
Accuracy of a Polymerase Chain Reaction-based Assay for Detection of Pneumococcal Bacteremia in Children
Isaacman, D. J., Zhang, Y., REYNOLDS, E. A., et al. (1998) · PEDIATRICS · doi:10.1542/peds.101.5.813 · Cited by 40
Sensitive and Specific Target Sequences Selected from Retrotransposons of Schistosoma japonicum for the Diagnosis of Schistosomiasis
Guo, J.-J., Zheng, H., Xu, J., et al. (2012) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0001579 · Cited by 39
Usefulness of Seminested Polymerase Chain Reaction for Screening Blood Donors at Risk for Malaria in Spain
Benito, A., & Rubio, J. M. (2001) · Emerging infectious diseases · doi:10.3201/eid0706.010632 · Cited by 39
“The five PCR-positive blood specimens were negative by microscopy (7) (we assumed that 400 microscopic fields are equivalent to 1 μL of blood).”
DNA QUALITY AND ACCURACY OF AVIAN MALARIA PCR DIAGNOSTICS: A REVIEW
Freed, L. A., & Cann, R. L. (2006) · Ornithological Applications · doi:10.1650/0010-5422(2006)108[459:dqaaoa]2.0.co;2 · Cited by 37
“Human malaria data from Barker et al. 1992, Brown et al. 1992, Sethabutr et al. 1992, Seesod et. al.”
Method
Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model
Suzuki, T., Osada, Y., Kumagai, T., et al. (2006) · Parasitology International · doi:10.1016/j.parint.2006.05.004 · Cited by 36
Bioluminescence DNA Hybridization Assay for Plasmodium falciparum Based on the Photoprotein Aequorin
Knecht, L. D., Davies, L., Rowe, L. A., et al. (2007) · Analytical Chemistry · doi:10.1021/ac0702847 · Cited by 36
Comparative assessment of conventional PCR with multiplex real-time PCR using SYBR Green I detection for the molecular diagnosis of imported malaria
FABRE, R., Berry, A., Morassin, B., & Magnaval, J. (2004) · Parasitology · doi:10.1017/s0031182003004219 · Cited by 34
“…than the conventional optical (thin and thick blood smear examination, Quantitative Buffy CoatTM [QBCTM]) or immunochromatographic techniques (Barker et al. 1989, 1992; Jaureguiberry et al. 1990; Brown et al. 1992; Snounou et al. 1993a, b ; Craig & Sharp, 1997; Humar et al. 1997; Makler,…”
Method
A comparison of thick smears, QBC Malaria, PCR and PATH Falciparum Malaria® Test Trip inPlasmodium falciparumdiagnosis
Gaye, O., Diouf, M., & Diallo, S. (1999) · Parasite · doi:10.1051/parasite/1999063273 · Cited by 33
Immunomagnetic purification to facilitate DNA diagnosis of Plasmodium falciparum
Seesod, N., Lunderberg, J., Hedrum, A., Åslund, L., Holder, A. A., Thaithong, S., & Uhlén, M. (1993) · Journal of Clinical Microbiology · doi:10.1128/jcm.31.10.2715-2719.1993 · Cited by 30
Use of PCR in the field
Barker, R. H. (1994) · Parasitology Today · doi:10.1016/0169-4758(94)90013-2 · Cited by 31
Self-citation
Theileria annulata: Carrier State and Immunitya
ILHAN, T., Williamson, S. M., Kirvar, E., et al. (1998) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1998.tb11040.x · Cited by 31
A method for the quantitative assessment of malaria parasite development in organs of the mammalian host
Hulier, E., Pétour, P., Snounou, G., et al. (1996) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(96)02584-4 · Cited by 31
Laboratory Detection of Malaria Antigens: a Strong Tool for Malaria Research, Diagnosis, and Epidemiology
Pluciński, M. M., Aidoo, M., & Rogier, E. W. (2021) · Clinical Microbiology Reviews · doi:10.1128/cmr.00250-20 · Cited by 33
A multiplex polymerase chain reaction for a differential diagnosis of Plasmodium falciparum and Plasmodium vivax
Kho, W.-G., Chung, J., Sim, E.-J., et al. (2003) · Parasitology International · doi:10.1016/s1383-5769(03)00028-x · Cited by 29
Strategies to Accelerate the Applicability of Gene Amplification Protocols for Pathogen Detection in Meat and Meat Products
Pilla, S. D., & Rickeb, S. C. (1995) · Critical Reviews in Microbiology · doi:10.3109/10408419509113542 · Cited by 27
Pyrimethamine/sulfadoxine combination in the treatment of uncomplicated falciparum malaria: relation between dihydropteroate synthase/dihydrofolate reductase genotypes, sulfadoxine plasma levels, and treatment outcome.
Khalil, I. F., Alifrangis, M., RØNN, A. M., et al. (2002) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2002.67.225 · Cited by 27
Post treatment sensitivity studies with the parasight®-F test for malaria diagnosis in Zimbabwe
Mharakurwa, S., & Shiff, C. (1997) · Acta Tropica · doi:10.1016/s0001-706x(97)00674-8 · Cited by 26
PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA
Li, F., Gasser, R. B., Lai, D., et al. (2006) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2006.03.009 · Cited by 25
“Samples were processed as described previously [42].”
Development and Evaluation of a Rapid Diagnostic Test for Plasmodium falciparum, P. vivax, and Mixed-Species Malaria Antigens
Lee, G., Jeon, E.-S., Le, D. T., et al. (2011) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2011.11-0265 · Cited by 25
Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolates
Chung, J., Chun, E.-H., Chun, J., et al. (2003) · Parasitology Research · doi:10.1007/s00436-002-0777-2 · Cited by 25
“For the parasite density estimation, it was assumed that there were 8,000 WBCs in 1 ll of blood (Baker et al. 1992).”
Background
Detection of circulating Trichinella spiralis larvae by polymerase chain reaction
Uparanukraw, P., & Morakote, N. (1996) · Parasitology Research · doi:10.1007/s004360050207 · Cited by 24
Development and optimization of polymerase chain reaction-based malaria diagnostic methods and their comparison with quantitative buffy coat assay.
Schindler, H. C., Montenegro, L. M. L., Montenegro, R. D. A., et al. (2001) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2001.65.355 · Cited by 24
Plasmodium vivax: parasitemia determination by real-time quantitative PCR in Aotus monkeys
Polanco, J. C., Rodrı́guez, J. A., Corredor, V., & Patarroyo, M. A. (2002) · Experimental Parasitology · doi:10.1016/s0014-4894(02)00010-3 · Cited by 23
Epidemiologic tools for malaria surveillance in an urban setting of low endemicity along the Colombian Pacific coast.
Carrasquilla, G., Banguero, M., Sánchez, P., Carvajal, F., Barker, R. H., Gervais, G. W., Algarin, E., & Serrano, A. E. (2000) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2000.62.132 · Cited by 23
Self-citation
New insight-guided approaches to detect, cure, prevent and eliminate malaria
Kumar, S., Kumari, R., & Pandey, R. (2014) · PROTOPLASMA · doi:10.1007/s00709-014-0697-x · Cited by 23
Absence of Asymptomatic Malaria Infections in Previously High Endemic Areas of Sri Lanka
Fernando, S. D., Abeyasinghe, R. R., Galappaththy, G. N., et al. (2009) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.2009.09-0042 · Cited by 22
DNA Diagnosis of Ovale Malaria and Malariae Malaria Using Microtiter Plate-Hybridization
Arai, M., Kunisada, K., Kawai, S., Kimura, M., & Wataya, Y. (1994) · Nucleosides and Nucleotides · doi:10.1080/15257779408012158 · Cited by 21
Malaria prevalence defined by microscopy, antigen detection, DNA amplification and total nucleic acid amplification in a malaria‐endemic region during the peak malaria transmission season
Waitumbi, J. N., Gerlach, J., Afonina, I. A., Anyona, S. B., Koros, J. N., Siangla, J., Ankoudinova, I., Singhal, M. C., Watts, K., Polhemus, M. E., et al. (2011) · Tropical Medicine & International Health · doi:10.1111/j.1365-3156.2011.02773.x · Cited by 20
Nucleic Acid Extraction from Clinical Specimens for PCR Applications
Aygan, A. (2006) · DergiPark (Istanbul University) · Cited by 18
Laboratory diagnosis of malaria in nonhuman primates
Ameri, M. (2010) · Veterinary Clinical Pathology · doi:10.1111/j.1939-165x.2010.00217.x · Cited by 18
Evaluation of the polymerase chain reaction analysis for diagnosis of falciparum malaria in Delhi, India
Nandwani, S., Mathur, M., & Rawat, S. (2005) · Indian Journal of Medical Microbiology · doi:10.4103/0255-0857.16590 · Cited by 18
Transfusion-Related Transmissible Diseases: Detection by Polymerase Chain Reaction–Amplified Genes of the Microbial Agents
Vyas, G. N., Yang, G., & Murphy, E. L. (1994) · Transfusion Medicine Reviews · doi:10.1016/s0887-7963(94)70117-6 · Cited by 18
Comparison of two nested PCR methods for the detection of human malaria.
Anthony, C., Mahmud, R., Lau, Y. L., et al. (2013) · PubMed · Cited by 16
Detection of African horse sickness virus in the blood of experimentally infected horses: comparison of virus isolation and a PCR assay
Sailleau, C., Moulay, S., Crucière, C., et al. (1997) · Research in Veterinary Science · doi:10.1016/s0034-5288(97)90195-8 · Cited by 15
Detecting Plasmodium falciparum in community surveys: a comparison of Paracheck Pf® Test and ICT Malaria Pf® Cassette Test to polymerase chain reaction in Mutasa District, Zimbabwe
Mudare, N., Zingoni, Z. M., Makuwaza, A., Mamini, E., Munyati, S. S., Gwanzura, L., Midzi, N., Mutambu, S. L., Mason, P., Kobayashi, T., et al. (2021) · Malaria Journal · doi:10.1186/s12936-020-03536-7 · Cited by 12
High prevalence of malaria infection in Amazonas State, Venezuela.
Rodulfo, H., de Donato, M., Quijada, I., & Pena, A. (2007) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s0036-46652007000200003 · Cited by 19
DNA Quality and Accuracy of Avian Malaria PCR Diagnostics: A Review
Freed, L. A., & Cann, R. L. (2006) · Ornithological Applications · doi:10.1093/condor/108.2.459 · Cited by 14
Review on evaluations of currently available blood-culture systems
Rohner, P., & Auckenthaler, R. (1999) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.1999.tb00429.x · Cited by 13
Le risque de paludisme transfusionnel confronté à celui de la mutité biologique : deux données irréconciliables ?
Garraud, O., Relave, J., Flori, P., et al. (2004) · Transfusion Clinique et Biologique · doi:10.1016/j.tracli.2004.02.003 · Cited by 13
A Colorimetric DNA Diagnostic Method for Falciparum Malaria and Vivax Malaria: A Field Trial in the Solomon Islands
Arai, M., Kunisada, K., Kim, H., Miyake, H., Mizukoshi, C., Kakutani, T., Yamane, A., Nakagami, S., Kawai, S., Nakano, H., et al. (1996) · Nucleosides and Nucleotides · doi:10.1080/07328319608002417 · Cited by 13
Comparative evaluation of microscopy and polymerase chain reaction (PCR) for the diagnosis in suspected malaria patients of Nepal.
Parajuli, K., Hanchana, S., Inwong, M., Pukrittayakayamee, S., & Ghimire, P. (2009) · PubMed · Cited by 13
Diagnóstico molecular da malária em uma unidade de atenção terciária na Amazônia Brasileira
Costa, M. R. F., Vieira, P. P., Ferreira, C. D. O., et al. (2008) · Revista da Sociedade Brasileira de Medicina Tropical · doi:10.1590/s0037-86822008000400011 · Cited by 12
PCR amplification reactions in parasitology
Smits, H. L., & Hartskeerl, R. A. (1995) · Journal of Microbiological Methods · doi:10.1016/0167-7012(95)00032-g · Cited by 12
Rodent Plasmodium-infected red blood cells: Imaging their fates and interactions within their hosts
Claser, C., Malleret, B., Peng, K., Bakočević, N., Gun, S. Y., Russell, B., Ng, L. G., & Rénia, L. (2013) · Parasitology International · doi:10.1016/j.parint.2013.07.012 · Cited by 11
“[39] [42] [43] [45] [46] [47] [69] [70] [71] [72] [73] [77] [78] [80] [81] [82] [83] [86]”
Characteristics of Imported Malaria and Species of Plasmodium Involved in Shandong Province, China (2012-2014)
Xu, C., Wei, Q., Li, J., Xiao, T., Yin, K., Zhao, C. L., Wang, Y.-B., Kong, X.-L., Zhao, G.-H., Sun, H. Z., et al. (2016) · Korean Journal of Parasitology · doi:10.3347/kjp.2016.54.4.407 · Cited by 11
“Because sequences targeting 18S rRNA gene are highly conserved and specific to malaria species, nested PCR is considered to be more sensitive and specific than microscopy, especially in cases with low parasitemia levels or mixed infections [23,24].”
Background
Use of reverse transcriptase-polymerase chain reaction (RT-PCR) and dot-blot hybridisation for the detection and identification of African horse sickness virus nucleic acids
Zientara, S., Sailleau, C., Moulay, S., et al. (1998) · Springer eBooks · doi:10.1007/978-3-7091-6823-3_28 · Cited by 11
Utility of nested polymerase chain reaction over the microscopy and immuno-chromatographic test in the detection of Plasmodium species and their clinical spectrum
Ranjan, P., & Ghoshal, U. (2016) · Parasitology Research · doi:10.1007/s00436-016-5098-y · Cited by 10

Authors: Barker, R. H., Banchongaksorn, T., Courval, J. M., Suwonkerd, W., Rimwungtragoon, K., & Wirth, D. F.
Affiliations: Harvard University (US)
ORCID: Wirth, Dyann F.
Venue: The American Journal of Tropical Medicine and Hygiene
Publisher: American Society of Tropical Medicine and Hygiene
Published: 1992-04
Volume: 46
Issue: 4
Pages: 416-426
ISSN: 0002-9637 (ISSN-L)
DOI: 10.4269/ajtmh.1992.46.416
OpenAlex: W2411788447
Cited by: 148
References: 0
Citations by year: 2023: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 7 · 2012: 3
FWCI: 13.83
Type: journal article
Language: en
Open access: closed
Locations: American Journal of Tropical Medicine and Hygiene (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
Keywords: Plasmodium falciparum, malaria diagnosis, PCR inhibitors, sensitivity and specificity
MeSH terms: Animals, Base Sequence, DNA, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmodium falciparum, Sensitivity and Specificity, Blotting, Southern, DNA Probes, DNA, Protozoan, Polymerase Chain Reaction, Malaria, Falciparum

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A Simple Method to Detect Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cited by

Related

Metadata

Record

About these checks