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Quantitative Magnetic Particle Imaging Monitors the Transplantation, Biodistribution, and Clearance of Stem Cells In Vivo
Open accessAbstract
Stem cell therapies have enormous potential for treating many debilitating diseases, including heart failure, stroke and traumatic brain injury. For maximal efficacy, these therapies require targeted cell delivery to specific tissues followed by successful cell engraftment. However, targeted delivery remains an open challenge. As one example, it is common for intravenous deliveries of mesenchymal stem cells (MSCs) to become entrapped in lung microvasculature instead of the target tissue. Hence, a robust, quantitative imaging method would be essential for developing efficacious cell therapies. Here we show that Magnetic Particle Imaging (MPI), a novel technique that directly images iron-oxide nanoparticle-tagged cells, can longitudinally monitor and quantify MSC administration in vivo. MPI offers near-ideal image contrast, depth penetration, and robustness; these properties make MPI both ultra-sensitive and linearly quantitative. Here, we imaged, for the first time, the dynamic trafficking of intravenous MSC administrations using MPI. Our results indicate that labeled MSC injections are immediately entrapped in lung tissue and then clear to the liver within one day, whereas standard iron oxide particle (Resovist) injections are immediately taken up by liver and spleen. Longitudinal MPI-CT imaging also indicated a clearance half-life of MSC iron oxide labels in the liver at 4.6 days. Finally, our ex vivo MPI biodistribution measurements of iron in liver, spleen, heart, and lungs after injection showed excellent agreement (R(2) = 0.943) with measurements from induction coupled plasma spectrometry. These results demonstrate that MPI offers strong utility for noninvasively imaging and quantifying the systemic distribution of cell therapies and other therapeutic agents.
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“Another study used quantitative MPI to monitor transplantation, bio-distribution, and clearance of ferucarbotran-labelled human stem cells in vivo [151].”
Background
Metadata
- Authors
- Zheng, B., von See, M. P., Yu, E., Gunel, B., Lu, K., Vazin, T., Schaffer, D. V., Goodwill, P. W., & Conolly, S. M.
- Affiliations
- University of California, Berkeley (US)
- Corresponding author
- Zheng, Bo
- ORCID
- Venue
- Theranostics
- Publisher
- Ivyspring International Publisher
- Published
- 2016
- Volume
- 6
- Issue
- 3
- Pages
- 291-301
- ISSN
- 1838-7640 (ISSN-L)
- PMC
- PMC4737718
- OpenAlex
- W2275153700
- Cited by
- 322
- References
- 53
- Citations by year
- 2026: 18 · 2025: 25 · 2024: 29 · 2023: 36 · 2022: 37 · 2021: 35 · 2020: 34 · 2019: 30 · 2018: 30 · 2017: 35 · 2016: 13
- FWCI
- 12.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Theranostics (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- tub.dok (Hamburg University of Technology) (submittedVersion, cc-by-nc)
- TUHH Open Research
- Funders
- W. M. Keck Foundation (009323)
- ACT Government
- Siebel Scholars Foundation
- National Institutes of Health (009323, NIH-R01)
- National Institute of Mental Health (R24MH106053)
- National Institute of Biomedical Imaging and Bioengineering (R01EB019458, R01EB013689)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- magnetic particle imaging, mesenchymal stem cells, stem cell transplantation, cell biodistribution, cell clearance, stem cell therapy
- MeSH terms
- Animals, Diagnostic Imaging, Female, Ferric Compounds, Humans, Magnetics, Rats, Inbred F344, Staining and Labeling, Tissue Distribution, Mesenchymal Stem Cell Transplantation, Mice, Nanoparticles, Administration, Intravenous
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