The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics
Open accessAbstract
Exosomes are small extracellular vesicles with diameters of 30-150 nm. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances. Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes. In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. In addition, from the perspective of exosome-based diagnosis and therapeutics, we emphasize the issue of quantitative exosome and microvesicle separation.
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Cites
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Reassessment of exosome composition
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Exosomes: biogenesis, biologic function and clinical potential
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Microfluidic isolation and transcriptome analysis of serum microvesicles
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Self-citationEvidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
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Production of adeno-associated virus vectors for in vitro and in vivo applications
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Cited by
Exosome Processing and Characterization Approaches for Research and Technology Development
Lai, J. J., Chau, Z. L., Chen, S., Hill, J. J., Korpany, K. V., Liang, N., Lin, L., Lin, Y., Liu, J. K., Liu, Y., et al. (2022) · Advanced Science · doi:10.1002/advs.202103222 · Cited by 623
Self-citationReview on Strategies and Technologies for Exosome Isolation and Purification
Chen, J., Li, P., Zhang, T., Xu, Z., Huang, X., Wang, R., & Du, L. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.811971 · Cited by 585
The evolving translational potential of small extracellular vesicles in cancer
Möller, A., & Lobb, R. J. (2020) · Nature Reviews Cancer · doi:10.1038/s41568-020-00299-w · Cited by 544
Engineered Extracellular Vesicles for Cancer Therapy
Zhang, X., Zhang, H., Gu, J., Zhang, J., Shi, H., Qian, H., Wang, D., Xu, W., Pan, J., & Santos, H. A. (2021) · Advanced Materials · doi:10.1002/adma.202005709 · Cited by 428
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Metadata
- Authors
- Yang, D., Zhang, W., Zhang, H., Zhang, F., Chen, L., Ma, L., Larcher, L. M., Chen, S., Liu, N., Zhao, Q., Tran, P. H. L., Chen, C., Veedu, R. N., & Wang, T.
- Venue
- Theranostics
- Publisher
- Ivyspring International Publisher
- Published
- 2020
- Volume
- 10
- Issue
- 8
- Pages
- 3684-3707
- ISSN
- 1838-7640 (ISSN-L)
- OpenAlex
- W3008588181
- Cited by
- 1,023
- References
- 10
- FWCI
- 32.16
- Type
- journal article
- Language
- en
- Open access
- gold
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- Yang, D., Zhang, W., Zhang, H., Zhang, F., Chen, L., Ma, L., Larcher, L. M., Chen, S., Liu, N., Zhao, Q., Tran, P. H. L., Chen, C., Veedu, R. N., & Wang, T. (2020). Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics. Theranostics. https://doi.org/10.7150/thno.41580
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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