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Cited by 585Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryMicrofluidic and Capillary Electrophoresis Applications

Review on Strategies and Technologies for Exosome Isolation and Purification

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Chen, Jiaci, Li, Peilong, Zhang, Taiyi, Xu, Zhipeng, Huang, Xiaowen, Wang, Ruiming, Du, Lutao·2022·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2021.811971PMC PMC8766409
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Abstract

Exosomes, a nano-sized subtype of extracellular vesicles secreted from almost all living cells, are capable of transferring cell-specific constituents of the source cell to the recipient cell. Cumulative evidence has revealed exosomes play an irreplaceable role in prognostic, diagnostic, and even therapeutic aspects. A method that can efficiently provide intact and pure exosomes samples is the first step to both exosome-based liquid biopsies and therapeutics. Unfortunately, common exosomal separation techniques suffer from operation complexity, time consumption, large sample volumes and low purity, posing significant challenges for exosomal downstream analysis. Efficient, simple, and affordable methods to isolate exosomes are crucial to carrying out relevant researches. In the last decade, emerging technologies, especially microfluidic chips, have proposed superior strategies for exosome isolation and exhibited fascinating performances. While many excellent reviews have overviewed various methods, a compressive review including updated/improved methods for exosomal isolation is indispensable. Herein, we first overview exosomal properties, biogenesis, contents, and functions. Then, we briefly outline the conventional technologies and discuss the challenges of clinical applications of these technologies. Finally, we review emerging exosomal isolation strategies and large-scale GMP production of engineered exosomes to open up future perspectives of next-generation Exo-devices for cancer diagnosis and treatment.

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  • EVs can be obtained from MSCs using various methods, including ultracentrifugation, which provides a substantial amount of EVs with consistent purity and reproducibility.

    “EVs can be obtained from MSCs using various methods, including ultracentrifugation, which provides a substantial amount of EVs with consistent purity and reproducibility, allowing for reliable in vitro and in vivo studies [30,36,45,46,47,48].”

    From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [47]

    The Ultracentrifugation section confirms that the method isolates exosomes, but says its repeatability is “poor/unstable” and that impurities remain in the precipitate. Table 1 rates its yield as low and purity as medium, contrary to the claimed substantial amount, consistent purity, and reproducibility. The provided passages do not specify MSC-derived EVs. (LLM, conf=0.90)

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Cited by

Sorted by citations

Authors
Chen, J., Li, P., Zhang, T., Xu, Z., Huang, X., Wang, R., & Du, L.
Affiliations
  • Qilu University of Technology (CN)
  • Shandong University (CN)
  • University of Bristol (GB)
Corresponding author
  • Huang, Xiaowen
  • Wang, Ruiming
  • Du, Lutao
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2022-01-05
Volume
9
Pages
811971-811971
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2021.811971
PMC
PMC8766409
OpenAlex
W4206315850
Cited by
585
References
7
Citations by year
2026: 89 · 2025: 165 · 2024: 153 · 2023: 124 · 2022: 54
FWCI
12.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China
  • Natural Science Foundation of Shandong Province
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
exosomes, extracellular vesicles, exosome isolation, microfluidics, liquid biopsy, engineered exosomes, GMP production, exosome biogenesis, cancer diagnosis, cancer therapy, exosome therapy

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