Plasticity-dependent, full detonation at hippocampal mossy fiber-CA3 pyramidal neuron synapses
Open accessAbstract
Mossy fiber synapses on CA3 pyramidal cells are 'conditional detonators' that reliably discharge postsynaptic targets. The 'conditional' nature implies that burst activity in dentate gyrus granule cells is required for detonation. Whether single unitary excitatory postsynaptic potentials (EPSPs) trigger spikes in CA3 neurons remains unknown. Mossy fiber synapses exhibit both pronounced short-term facilitation and uniquely large post-tetanic potentiation (PTP). We tested whether PTP could convert mossy fiber synapses from subdetonator into detonator mode, using a recently developed method to selectively and noninvasively stimulate individual presynaptic terminals in rat brain slices. Unitary EPSPs failed to initiate a spike in CA3 neurons under control conditions, but reliably discharged them after induction of presynaptic short-term plasticity. Remarkably, PTP switched mossy fiber synapses into full detonators for tens of seconds. Plasticity-dependent detonation may be critical for efficient coding, storage, and recall of information in the granule cell-CA3 cell network.
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Chemical potentiation markedly regulates the conditional detonation properties of the synapse.
“Thus, chemical potentiation markedly regulates the conditional detonation properties of the synapse [25,50,51].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [51]
The Results report that potentiation markedly changed detonation: after high-frequency stimulation induced post-tetanic potentiation, the probability of a spike from the first stimulus rose from 0.14 to 0.71 at its peak. The paper supports regulation of detonation by potentiation, but the reported intervention was high-frequency stimulation, not chemical potentiation. (LLM, conf=0.86)
Experimental — accuracy not yet measuredChemical potentiation can switch the hippocampal mossy fiber synapse from a subdetonation into a detonation mode.
“The unitary EPSP recordings (Fig 2) provide direct evidence for this hypothesis, demonstrating that chemical potentiation can switch the hippocampal mossy fiber synapse from a subdetonation into a detonation mode [50,51].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [51]
The Results report that post-tetanic potentiation switched mossy fiber–CA3 synapses from subdetonator to full detonator mode. But the potentiation was induced by high-frequency electrical stimulation (Figure 3), not chemical potentiation; Figure 2 concerns facilitation during a short stimulus train. (LLM, conf=0.88)
Experimental — accuracy not yet measuredHippocampal mossy fiber bouton synapses have low initial release probability.
“The hippocampal MFB synapses are synapses with low initial Pr [51,69,70].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [51]
Facilitation and PTP may lead to conditional detonation or plasticity-dependent detonation at this synapse.
“Previous work showed that facilitation and PTP may lead to conditional detonation or plasticity-dependent detonation at this synapse [21,51].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [51]
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- Authors
- Vyleta, N. P., Borges-Merjane, C., & Jonas, P.
- Affiliations
- Institute of Science and Technology Austria (AT)
- Institute of Science and Technology Austria, Klosterneuburg, Austria
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2016-10-25
- Volume
- 5
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC5079747
- OpenAlex
- W2535386109
- Cited by
- 91
- References
- 9
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 10 · 2023: 8 · 2022: 7 · 2021: 14 · 2020: 10 · 2019: 13 · 2018: 9 · 2017: 5 · 2016: 1
- FWCI
- 5.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health (2T32HL083808)
- People Programme (291734)
- European Research Council (268548, 692692)
- Austrian Science Fund (FWF P 24909-B24)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- mossy fiber synapses, CA3 pyramidal cells, post-tetanic potentiation, short-term plasticity, excitatory postsynaptic potentials, dentate gyrus granule cells, hippocampus, synaptic facilitation
- MeSH terms
- Animals, Neuronal Plasticity, Synapses, Pyramidal Cells, Mossy Fibers, Hippocampal, Excitatory Postsynaptic Potentials, Rats, CA3 Region, Hippocampal
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