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Cited by 46Wnt/β-catenin signaling in development and cancerCellular Mechanics and InteractionsHippo pathway signaling and YAP/TAZ

Increasing β-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis

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Benham-Pyle, Blair W, Sim, Joo Yong, Hart, Kevin C, Pruitt, Beth L, Nelson, William James·2016·eLife
DOI 10.7554/elife.19799PMC PMC5104517
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Abstract

Mechanical force and Wnt signaling activate β-catenin-mediated transcription to promote proliferation and tissue expansion. However, it is unknown whether mechanical force and Wnt signaling act independently or synergize to activate β-catenin signaling and cell division. We show that mechanical strain induced Src-dependent phosphorylation of Y654 β-catenin and increased β-catenin-mediated transcription in mammalian MDCK epithelial cells. Under these conditions, cells accumulated in S/G2 (independent of DNA damage) but did not divide. Activating β-catenin through Casein Kinase I inhibition or Wnt3A addition increased β-catenin-mediated transcription and strain-induced accumulation of cells in S/G2. Significantly, only the combination of mechanical strain and Wnt/β-catenin activation triggered cells in S/G2 to divide. These results indicate that strain-induced Src phosphorylation of β-catenin and Wnt-dependent β-catenin stabilization synergize to increase β-catenin-mediated transcription to levels required for mitosis. Thus, local Wnt signaling may fine-tune the effects of global mechanical strain to restrict cell divisions during tissue development and homeostasis.

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  • A role for Wnt signaling during M phase has been suggested based on high Wnt/β-catenin signaling activity during cell division.

    “A role for Wnt signaling during M phase has also been suggested based on high Wnt/β-catenin signaling activity during cell division, through an unknown mechanism (39, 40).”

    From Wnt signaling regulates hepatocyte cell division by a transcriptional repressor cascade (2022) · cites this paper as [40]

    The Discussion reports that higher Wnt3A/β-catenin activity drove mechanically strained cells from G2 into mitosis, supporting a suggested role in mitotic progression. It does not establish that signaling activity was high during M phase itself; the reported activity concerns progression into mitosis. (LLM, conf=0.83)

    Experimental — accuracy not yet measured

  • High Wnt/βcatenin signaling activity during cell division suggests a link between Wnt signaling and mitosis.

    “High Wnt/βcatenin signaling activity during cell division has suggested a link between Wnt signaling and mitosis through unknown mechanisms (Benham-Pyle et al. 2016;Niehrs and Acebron 2012).”

    From Hepatocyte Cell Cycle Progression Depends on a Transcriptional Repressor Cascade Downstream of Wnt Signaling (2021) · cites this paper as Benham-Pyle et al. 2016

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Cited by

Sorted by citations

Authors
Benham-Pyle, B. W., Sim, J. Y., Hart, K. C., Pruitt, B. L., & Nelson, W. J.
Affiliations
  • Stanford University (US)
  • Stanford Cardiovascular Institute (US)
Corresponding author
Nelson, William James
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2016-10-26
Volume
5
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.19799
PMC
PMC5104517
OpenAlex
W2541569086
Cited by
46
References
2
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 10 · 2021: 10 · 2020: 5 · 2019: 6 · 2018: 7 · 2017: 1
FWCI
2.43
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
Funders
  • National Science Foundation (1136790, Graduate Student Fellowship, DGE-114747)
  • National Institutes of Health (T32GM007276, 11R35GM118064-01)
  • Stanford University (Bio-X Graduate Fellowship, Lieberman Graduate Fellowship)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
Keywords
β-catenin, Wnt3a, mechanical strain, cell cycle progression, mitosis, Src phosphorylation, casein kinase 1, β-catenin stabilization, Wnt/β-catenin signaling, mechanical forces, MDCK cells, tissue expansion
MeSH terms
Animals, Dogs, Mitosis, Phosphorylation, Protein Processing, Post-Translational, src-Family Kinases, beta Catenin, Mechanical Phenomena, Wnt Signaling Pathway, Wnt3A Protein, Madin Darby Canine Kidney Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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