Cited by 106Hydrogels: synthesis, properties, applicationsOsteoarthritis Treatment and MechanismsBone Tissue Engineering Materials

Mussel‐Inspired Tough Hydrogel with In Situ Nanohydroxyapatite Mineralization for Osteochondral Defect Repair

Gan, Donglin, Wang, Zhixiong, Xie, Chaoming, Wang, Xiao, Xing, Wensi, Ge, Xiang, Yuan, Huipin, Wang, Kefeng +2 more·2019·Advanced Healthcare Materials

Abstract

Repairing osteochondral defects is a considerable challenge because it involves the breakdown of articular cartilage and underlying bone. Traditional hydrogels with a homogenized single-layer structure cannot fully restore the function of osteochondral cartilage tissue. In this study, a mussel-inspired hydrogel with a bilayer structure is developed to repair osteochondral defects. The hydrogel is synthesized by simultaneously polymerizing two layers using a one-pot method. The resulting upper and lower gelatin methacryloyl-polydopamine hydrogel layers are used as cartilage and subchondral bone repair layers, respectively. Polydopamine-induced hydroxyapatite in situ mineralization takes place in the lower layer to mimic the structure of subchondral bone. The bilayer hydrogel exhibits good mechanical properties for the synergistic effect of covalent and noncovalent bonds, as well as nanoreinforcement of mineralized hydroxyapatite. To improve the tissue-inducibility of hydrogels, transforming growth factor β 3 is immobilized in the upper layer to induce cartilage regeneration, while bone morphogenetic protein 2 is immobilized in the lower layer to induce bone regeneration. Bone and cartilage repair performance of the hydrogel is examined by implantation into a full-thickness cartilage defect of a rabbit knee joint. The bilayer-structure hydrogel promotes regeneration of osteochondral tissue, thus providing a new option for repair of osteochondral defects.

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Crossref · 10.1002/adhm.201901103

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Authors
Gan, D., Wang, Z., Xie, C., Wang, X., Xing, W., Ge, X., Yuan, H., Wang, K., Tan, H., & Lu, X.
Affiliations
  • Southwest Jiaotong University (CN)
  • Tianjin University (CN)
  • Sichuan University (CN)
  • Shenzhen University (CN)
Corresponding author
  • Tan, Hui
  • Lu, Xiong
Venue
Advanced Healthcare Materials
Publisher
Wiley
Published
2019-10-14
Volume
8
Issue
22
Pages
e1901103-e1901103
ISSN
  • 2192-2640 (ISSN-L)
  • 2192-2659
OpenAlex
W2979683011
Cited by
106
References
44
Citations by year
2026: 9 · 2025: 12 · 2024: 15 · 2023: 19 · 2022: 19 · 2021: 20 · 2020: 12
FWCI
4.40
Type
journal article
Language
en
Open access
closed
Locations
  • Advanced Healthcare Materials (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Basic Research Program of China (2016YFB0700802)
  • National Natural Science Foundation of China (81671824, 51773119, 31700841)
  • Fundamental Research Funds for the Central Universities (2682019JQ03)
  • Natural Science Foundation of Tianjin City (18JCYBJC19500)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
mussel-inspired hydrogel, bilayer hydrogel, osteochondral repair, hydroxyapatite mineralization, gelatin methacryloyl, polydopamine, subchondral bone regeneration, articular cartilage regeneration, tissue engineering scaffolds, rabbit knee
MeSH terms
Animals, Bone Regeneration, Cartilage, Articular, Cells, Cultured, Female, Male, Microscopy, Electron, Scanning, Rabbits, Durapatite, Chondrocytes, Hydrogels, Tissue Engineering

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