Mussel‐Inspired Tough Hydrogel with In Situ Nanohydroxyapatite Mineralization for Osteochondral Defect Repair
Abstract
Repairing osteochondral defects is a considerable challenge because it involves the breakdown of articular cartilage and underlying bone. Traditional hydrogels with a homogenized single-layer structure cannot fully restore the function of osteochondral cartilage tissue. In this study, a mussel-inspired hydrogel with a bilayer structure is developed to repair osteochondral defects. The hydrogel is synthesized by simultaneously polymerizing two layers using a one-pot method. The resulting upper and lower gelatin methacryloyl-polydopamine hydrogel layers are used as cartilage and subchondral bone repair layers, respectively. Polydopamine-induced hydroxyapatite in situ mineralization takes place in the lower layer to mimic the structure of subchondral bone. The bilayer hydrogel exhibits good mechanical properties for the synergistic effect of covalent and noncovalent bonds, as well as nanoreinforcement of mineralized hydroxyapatite. To improve the tissue-inducibility of hydrogels, transforming growth factor β 3 is immobilized in the upper layer to induce cartilage regeneration, while bone morphogenetic protein 2 is immobilized in the lower layer to induce bone regeneration. Bone and cartilage repair performance of the hydrogel is examined by implantation into a full-thickness cartilage defect of a rabbit knee joint. The bilayer-structure hydrogel promotes regeneration of osteochondral tissue, thus providing a new option for repair of osteochondral defects.
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GelMA-PDA/HA tested in vivo for knee joint defect reparation in rabbits showed higher subchondral bone formation compared to pristine GelMA.
“In addition, the GelMA-PDA/HA was also tested in vivo for knee joint defect reparation in rabbits, showing higher subchondral bone formation compared to pristine GelIMA.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
The GelMA-PDA/HA bilayer hydrogel enhanced adhesion and differentiation of bone mesenchymal stem cells in vitro.
“This hydrogel enhanced the adhesion and differentiation of bone mesenchymal stem cells in vitro.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
The subchondral bone regeneration capacity of bilayer hydrogels was 20% higher compared to pure GelMA hydrogels.
“They observed that the subchondral bone regeneration capacity of bilayer hydrogels was 20% higher compared to pure GelMA hydrogels”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
The GelMA-PDA/HA bilayer hydrogel had improved stability.
“Besides improved mechanical strength, the swelling ratio (~70% reduction from GelMA hydrogels) and the stability of the GelMA-PDA/HA bilayer hydrogel were improved.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
The GelMA-PDA/HA bilayer hydrogel had a swelling ratio ~70% lower than GelMA hydrogels.
“Besides improved mechanical strength, the swelling ratio (~70% reduction from GelMA hydrogels) and the stability of the GelMA-PDA/HA bilayer hydrogel were improved.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
The GelMA-PDA/HA bilayer hydrogel had improved mechanical strength.
“Besides improved mechanical strength, the swelling ratio (~70% reduction from GelMA hydrogels) and the stability of the GelMA-PDA/HA bilayer hydrogel were improved.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
Incorporation of growth factors in bilayer hydrogels further improved bone tissue regeneration by 20%.
“and the incorporation of growth factors in bilayer hydrogels further improved bone tissue regeneration by 20%.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [71]
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- Authors
- Gan, D., Wang, Z., Xie, C., Wang, X., Xing, W., Ge, X., Yuan, H., Wang, K., Tan, H., & Lu, X.
- Affiliations
- Southwest Jiaotong University (CN)
- Tianjin University (CN)
- Sichuan University (CN)
- Shenzhen University (CN)
- Corresponding author
- Tan, Hui
- Lu, Xiong
- ORCID
- Venue
- Advanced Healthcare Materials
- Publisher
- Wiley
- Published
- 2019-10-14
- Volume
- 8
- Issue
- 22
- Pages
- e1901103-e1901103
- ISSN
- 2192-2640 (ISSN-L)
- 2192-2659
- OpenAlex
- W2979683011
- Cited by
- 106
- References
- 44
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 15 · 2023: 19 · 2022: 19 · 2021: 20 · 2020: 12
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Update notices
- correction (2023-01-13)
- Open access
- closed
- Locations
- Advanced Healthcare Materials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Basic Research Program of China (2016YFB0700802)
- National Natural Science Foundation of China (81671824, 51773119, 31700841)
- Fundamental Research Funds for the Central Universities (2682019JQ03)
- Natural Science Foundation of Tianjin City (18JCYBJC19500)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- mussel-inspired hydrogel, bilayer hydrogel, osteochondral repair, hydroxyapatite mineralization, gelatin methacryloyl, polydopamine, subchondral bone regeneration, articular cartilage regeneration, tissue engineering scaffolds, rabbit knee
- MeSH terms
- Animals, Bone Regeneration, Cartilage, Articular, Cells, Cultured, Female, Male, Microscopy, Electron, Scanning, Rabbits, Durapatite, Chondrocytes, Hydrogels, Tissue Engineering
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