Oil extraction from microalgae for biodiesel production
Abstract
This study examines the performance of supercritical carbon dioxide (SCCO(2)) extraction and hexane extraction of lipids from marine Chlorococcum sp. for lab-scale biodiesel production. Even though the strain of Chlorococcum sp. used in this study had a low maximum lipid yield (7.1 wt% to dry biomass), the extracted lipid displayed a suitable fatty acid profile for biodiesel [C18:1 (∼63 wt%), C16:0 (∼19 wt%), C18:2 (∼4 wt%), C16:1 (∼4 wt%), and C18:0 (∼3 wt%)]. For SCCO(2) extraction, decreasing temperature and increasing pressure resulted in increased lipid yields. The mass transfer coefficient (k) for lipid extraction under supercritical conditions was found to increase with fluid dielectric constant as well as fluid density. For hexane extraction, continuous operation with a Soxhlet apparatus and inclusion of isopropanol as a co-solvent enhanced lipid yields. Hexane extraction from either dried microalgal powder or wet microalgal paste obtained comparable lipid yields.
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- Authors
- Halim, R., Gladman, B., Danquah, M. K., & Webley, P. A.
- Affiliations
- Monash University (AU)
- Corresponding author
- Halim, Ronald
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 102
- Issue
- 1
- Pages
- 178-185
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2032146813
- Cited by
- 683
- References
- 7
- Citations by year
- 2026: 14 · 2025: 17 · 2024: 20 · 2023: 26 · 2022: 37 · 2021: 44 · 2020: 46 · 2019: 56 · 2018: 51 · 2017: 58 · 2016: 53 · 2015: 66 · 2014: 66 · 2013: 62 · 2012: 40
- FWCI
- 19.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- eSpace (Curtin University) (submittedVersion)
- Funders
- Australian Research Council
- Department of Chemical Engineering, Monash University
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biodiesel Production and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- microalgae, Chlorococcum, biodiesel, supercritical CO2 extraction, hexane extraction, lipid extraction, fatty acid composition, mass transfer coefficient, Soxhlet extraction, extraction pressure and temperature, dielectric constant, fluid density
- MeSH terms
- Chlorophyta, Carbon Dioxide, Fatty Acids, Hexanes, Lipids, Oils, Pressure, Solvents, Temperature, Biomass, Biofuels
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