Neuroprotective Effects of Resveratrol on Damages of Mouse Cortical Neurons Induced by β-amyloid through Activation of SIRT1/Akt1 Pathway
Abstract
Resveratrol (3,5,4'-tihydroxy-trans-stilbene), a polyphenolic phytoalexin found in the skin and seeds of grapes, has been reported to possess a wide range of biological and pharmacological activities including antioxidant, anti-inflammatory, and antimutagenic effects. The present study intended to explore the neuroprotective effects of resveratrol against Aβ25-35 -induced neurotoxicity of cultured mouse cortical neurons and the possible mechanisms involved. For this purpose, mouse cortical neurons were cultured and exposed to 30 μM Aβ25-35 in the absence or presence of resveratrol (5, 10, and 25 μM). In addition, the potential contribution of the SIRT1/Akt1 neuroprotective pathway in resveratrol-mediated protection against Aβ25-35 -induced neurotoxicity was also investigated. The results showed that resveratrol dose-dependently increased cell viability and reduced the number of apoptotic cells as measured by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) activity assay, reactive oxygen species (ROS) activity assay, and Hoechst/PI double staining. Further study revealed that resveratrol through activation of SIRT1/Akt1 to avert apoptosis. These findings raise the possibility that resveratrol may be a potent therapeutic compound against the neurodegenerative diseases.
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Resveratrol modulates Aβ clearance through several mechanisms.
“to modulate the Aβ clearance through several mechanisms (Zhang et al., 2014)”
From Exploring the Therapeutic Potential of Phytochemicals in Alzheimer’s Disease: Focus on Polyphenols and Monoterpenes (2022) · cites this paper as Zhang et al., 2014
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- Authors
- Zhang, J., Feng, X., Wu, J., Xu, H., Li, G., Zhu, D., Yue, Q., Liu, H., Zhang, Y., Sun, D., Wang, H., & Sun, J.
- Affiliations
- Shandong University (CN)
- Corresponding author
- Sun, Jinhao
- Venue
- BioFactors
- Publisher
- Wiley
- Published
- 2013-10-17
- Volume
- 40
- Issue
- 2
- Pages
- 258-267
- ISSN
- 0951-6433 (ISSN-L)
- 1872-8081
- OpenAlex
- W1567421949
- Cited by
- 50
- References
- 56
- Citations by year
- 2026: 2 · 2024: 4 · 2023: 6 · 2022: 2 · 2021: 2 · 2020: 7 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 8 · 2014: 4
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- BioFactors (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of Shandong Province (ZR2010HM051)
- Shandong Provincial Science and Technology Development Plan (2011GSF11810)
- Specialized Research Fund for the Doctoral Program of Higher Education (20120131110039)
- Independent Innovation Foundation of Shandong University (2012TS113)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Keywords
- resveratrol, neuroprotection, amyloid-beta, amyloid beta 25-35, cortical neurons, SIRT1, AKT1, neurotoxicity, apoptosis, reactive oxygen species, neurodegenerative diseases, polyphenols
- MeSH terms
- Resveratrol, Animals, Cell Survival, Cells, Cultured, Drug Evaluation, Preclinical, Enzyme Activation, Neurons, Peptide Fragments, Stilbenes, Signal Transduction, Amyloid beta-Peptides, Apoptosis, Reactive Oxygen Species, Neuroprotective Agents, Proto-Oncogene Proteins c-akt, Mice, Sirtuin 1
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