Cited by 50Sirtuins and Resveratrol in MedicineAlzheimer's disease research and treatmentsNeurological Disorders and Treatments

Neuroprotective Effects of Resveratrol on Damages of Mouse Cortical Neurons Induced by β-amyloid through Activation of SIRT1/Akt1 Pathway

Zhang, Jing, Feng, Xiaowen, Wu, Jintao, Xu, Hongyan, Li, Guibao, Zhu, Dexiao, Yue, Qingwei, Liu, Haili +4 more·2014·BioFactors

Abstract

Resveratrol (3,5,4'-tihydroxy-trans-stilbene), a polyphenolic phytoalexin found in the skin and seeds of grapes, has been reported to possess a wide range of biological and pharmacological activities including antioxidant, anti-inflammatory, and antimutagenic effects. The present study intended to explore the neuroprotective effects of resveratrol against Aβ25-35 -induced neurotoxicity of cultured mouse cortical neurons and the possible mechanisms involved. For this purpose, mouse cortical neurons were cultured and exposed to 30 μM Aβ25-35 in the absence or presence of resveratrol (5, 10, and 25 μM). In addition, the potential contribution of the SIRT1/Akt1 neuroprotective pathway in resveratrol-mediated protection against Aβ25-35 -induced neurotoxicity was also investigated. The results showed that resveratrol dose-dependently increased cell viability and reduced the number of apoptotic cells as measured by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) activity assay, reactive oxygen species (ROS) activity assay, and Hoechst/PI double staining. Further study revealed that resveratrol through activation of SIRT1/Akt1 to avert apoptosis. These findings raise the possibility that resveratrol may be a potent therapeutic compound against the neurodegenerative diseases.

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Crossref · 10.1002/biof.1149

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Authors
Zhang, J., Feng, X., Wu, J., Xu, H., Li, G., Zhu, D., Yue, Q., Liu, H., Zhang, Y., Sun, D., Wang, H., & Sun, J.
Affiliations
Shandong University (CN)
Corresponding author
Sun, Jinhao
Venue
BioFactors
Publisher
Wiley
Published
2013-10-17
Volume
40
Issue
2
Pages
258-267
ISSN
  • 0951-6433 (ISSN-L)
  • 1872-8081
OpenAlex
W1567421949
Cited by
50
References
56
Citations by year
2026: 2 · 2024: 4 · 2023: 6 · 2022: 2 · 2021: 2 · 2020: 7 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 8 · 2014: 4
FWCI
2.64
Type
journal article
Language
en
Open access
closed
Locations
  • BioFactors (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Science Foundation of Shandong Province (ZR2010HM051)
  • Shandong Provincial Science and Technology Development Plan (2011GSF11810)
  • Specialized Research Fund for the Doctoral Program of Higher Education (20120131110039)
  • Independent Innovation Foundation of Shandong University (2012TS113)
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
Keywords
resveratrol, neuroprotection, amyloid-beta, amyloid beta 25-35, cortical neurons, SIRT1, AKT1, neurotoxicity, apoptosis, reactive oxygen species, neurodegenerative diseases, polyphenols
MeSH terms
Resveratrol, Animals, Cell Survival, Cells, Cultured, Drug Evaluation, Preclinical, Enzyme Activation, Neurons, Peptide Fragments, Stilbenes, Signal Transduction, Amyloid beta-Peptides, Apoptosis, Reactive Oxygen Species, Neuroprotective Agents, Proto-Oncogene Proteins c-akt, Mice, Sirtuin 1

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