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Cited by 282

The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides

Open access
Deo, Monika, Yu, Jenn‐Yah, Chung, Kwan‐Ho, Tippens, Melissa, Turner, David L.·2006·Developmental Dynamics

Abstract

We have developed an in situ hybridization procedure for the detection of microRNAs (miRNAs) in tissue sections from mouse embryos and adult organs. The method uses highly specific washing conditions for RNA oligonucleotide probes conjugated to a fluorescein hapten. We show that this method detects predominantly mature miRNAs rather than the miRNA precursors or primary transcripts. We have determined expression patterns for several miRNAs expressed in the developing and adult nervous system, including miR‐124a, miR‐9, miR‐92, and miR‐204. Whereas miR‐124a is expressed in neurons, miR‐9 is expressed in neural progenitors and some neurons, and miR‐204 is expressed in the choroid plexus, retinal pigment epithelium, and ciliary body. miR‐204 is located in an intron of the TRPM3 gene, and the TRPM3 mRNA is coexpressed with miR‐204 in the choroid plexus. We also find that primary transcripts for miR‐124a and miR‐9 genes are expressed in patterns similar to their respective mature miRNAs. The ability to visualize expression of specific miRNAs in embryos and tissues should aid studies on miRNA function. Developemental Dynamics 235:2538–2548, 2006. © 2006 Wiley‐Liss, Inc.

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Metadata

Authors
Deo, M., Yu, J., Chung, K., Tippens, M., & Turner, D. L.
Venue
Developmental Dynamics
Publisher
Wiley
Published
2006-05-30
Volume
235
Issue
9
Pages
2538-2548
ISSN
  • 1058-8388 (ISSN-L)
  • 1097-0177
OpenAlex
W2154909423
Cited by
282
References
12
FWCI
5.49
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides. https://app.cochat.ai/papers/10.1002/dvdy.20847
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Deo, M., Yu, J., Chung, K., Tippens, M., & Turner, D. L. (2006). Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides. Developmental Dynamics. https://doi.org/10.1002/dvdy.20847

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