Cited by 1,072Sperm and Testicular FunctionReproductive biology and impacts on aquatic speciesMale Reproductive Health Studies

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Open access

EVENSON, DONALD P., LARSON, KJERSTEN L., JOST, LORNA K.·2002·Journal of Andrology

DOI 10.1002/j.1939-4640.2002.tb02599.x

Open this paper in CoChat — free account Open PDF

Abstract

The Sperm Chromatin Structure Assay (SCSA) was first described in the December 5, 1980 issue of Science (Evenson et al, 1980a).The data described in that article showed a significant difference between proven fertile and subfertility or infertility in men and bulls and the susceptibility to denaturation of their sperm nuclear DNA.A subsequent study (Evenson et al, 1984) described SCSA data obtained from men with testicular cancer.The data were heterogeneous, and begged the question, what was SCSA measuring?Great effort has been spent over the past 15 years on animal model systems, mostly related to dose-response toxicology experiments and large animal fertility trials.The results from these studies showed that SCSA was highly dose-responsive to toxicants, highly repeatable, and provided meaningful biological information on sperm nuclear DNA defects.The extensive data from nonhumans (Evenson et al, 1985(Evenson et al, , 1986(Evenson et al, , 1989a(Evenson et al, ,b, 1993a(Evenson et al, ,b,c, 1994(Evenson et al, , 1995b;; Ballachey et

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1002/j.1939-4640.2002.tb02599.x

Cites

0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity
Rybouchkin, A. V., Van der Straeten, F., Quatacker, J., De Sutter, P., & Dhont, M. (1997) · Fertility and Sterility · doi:10.1016/s0015-0282(97)00378-6 · Cited by 169

Show all 1 references

Research works printed but not linked: 156

10.1242/dev.124.22.4615 · DOI
10.1016/S0022-5347(01)66350-X · DOI
10.1002/(SICI)1097-010X(19991101)284:6<696::AID-JEZ11>3.0.CO;2-E · DOI
10.1093/oxfordjournals.humrep.a136237 · DOI
10.1530/jrf.0.0940451 · DOI
10.1002/j.1939-4640.1988.tb01067.x · DOI
10.1095/biolreprod41.1.183 · DOI
Allen MJ, 1996, The chromatin structure of well‐spread demembranated human sperm nuclei revealed by atomic force microscopy, Scanning Microsc., 10, 989
10.1006/excr.1997.3719 · DOI
10.1111/j.1365-2605.1990.tb01052.x · DOI
Baccetti B., 1996, Apoptosis in human ejaculated sperm cells, J Submicrosc Cytol Pathol., 28, 587
10.1083/jcb.93.2.298 · DOI
10.1002/j.1939-4640.1988.tb01020.x · DOI
10.1095/biolreprod36.4.915 · DOI
10.1093/humrep/15.6.1338 · DOI
10.1074/jbc.272.32.19633 · DOI
10.1530/jrf.0.0330019 · DOI
10.1002/jez.1401880203 · DOI
10.1002/(SICI)1097-0320(19960401)23:4<263::AID-CYTO1>3.0.CO;2-I · DOI
10.1111/j.1399-3011.1994.tb00538.x · DOI
10.1095/biolreprod49.5.1083 · DOI
10.1093/molehr/2.3.139 · DOI
10.1095/biolreprod44.3.398 · DOI
Blanc‐Layrac G., 2000, Morphological and biochemical analysis of cell death in human ejaculated spermatozoa, Cell Mol Biol., 46, 187
10.1017/CBO9780511526954.010 · DOI
Calvin HI, 1971, Formation of disulphide bonds in the nucleus and accessory structures of mammalian spermatozoa during maturation in the epididymis, J Reprod Fertil, 13, 65
10.1002/1098-2795(20010401)58:4<437::AID-MRD12>3.0.CO;2-Q · DOI
10.1017/CBO9780511526954 · DOI
Claassens OE, 1992, The acridine orange test: determining the relationship between sperm morphology and fertilization in vitro, Hum Reprod., 7, 242, 10.1093/oxfordjournals.humrep.a137625 · DOI
10.3109/01485018808986737 · DOI
10.1111/j.1439-0272.1988.tb01058.x · DOI
10.1002/(SICI)1097-0320(19970101)27:1<1::AID-CYTO2>3.0.CO;2-L · DOI
Darzynkiewicz Z., 1990, Flow Cytometry and Sorting, 291
10.1016/0014-4827(75)90331-6 · DOI
Geyter C., 2000, Andrology: Male Reproductive Health and Dysfunction, 337
10.1002/j.1939-4640.1999.tb02544.x · DOI
DeJongeCJ.Paternal contributions to embryogenesis.Reprod Med Rev.2000;203–214. · DOI
10.1016/S0015-0282(98)00012-0 · DOI
10.1093/humrep/13.5.1235 · DOI
10.1093/humrep/15.3.657 · DOI
10.1002/j.1939-4640.1993.tb03366.x · DOI
10.1071/RD98023 · DOI
Evenson DP., 1999, Towards Reproductive Certainty: Fertility and Genetics Beyond, 313
10.1002/mrd.1080010409 · DOI
10.1002/em.2850140203 · DOI
10.1016/0041-008X(86)90447-3 · DOI
10.1002/cyto.990070107 · DOI
10.1126/science.7444440 · DOI
10.1007/BF00328394 · DOI
10.2527/1993.714955x · DOI
10.1002/cyto.990060311 · DOI
10.1111/j.1365-2184.1993.tb00015.x · DOI
Evenson DP, 1994, Methods in Cell Biology. Vol 42. Flow Cytometry, 159
10.1002/em.2850210208 · DOI
10.1016/0890-6238(91)90039-I · DOI
10.1002/j.1939-4640.2000.tb02142.x · DOI
10.1002/cyto.990190403 · DOI
10.1016/0890-6238(93)90019-4 · DOI
10.1093/humrep/14.4.1039 · DOI
Evenson DP, 2001, Proceedings of the Conference on the Teplice Program, 167
10.1016/S0022-5347(17)50109-3 · DOI
10.1177/31.1A_Suppl.6186729 · DOI
EvensonDP SailerBL JostLK.Relationship between stallion sperm deoxyribonucleic acid (DNA) susceptibility to denaturation in situ and presence of DNA strand breaks: implications for fertility and embryo viability.Biol Reprod. Monograph 1: equine reproduction VI.1995b:655–659. · DOI
10.1016/0093-691X(94)90174-H · DOI
10.1016/S0022-5320(78)80073-2 · DOI
10.1016/S0074-7696(08)62688-1 · DOI
Foresta C., 1989, Sperm nuclear chromatin heterogeneity in infertile subjects, Andrologia., 21, 384
10.1111/j.1365-2605.1992.tb01132.x · DOI
10.1002/(SICI)1097-0320(19970815)30:4<192::AID-CYTO6>3.0.CO;2-M · DOI
10.1046/j.1439-0272.1999.00290.x · DOI
10.1007/BF02110025 · DOI
10.1128/MCB.18.6.3350 · DOI
10.1007/BF00217120 · DOI
10.1002/j.1939-4640.1996.tb01783.x · DOI
10.1006/excr.1993.1182 · DOI
10.1111/j.1365-2605.1994.tb01247.x · DOI
10.1046/j.1365-2605.1997.00044.x · DOI
10.1006/excr.1995.1270 · DOI
10.3109/01485019408987794 · DOI
10.1007/978-1-4899-0089-0_10 · DOI
10.1093/humrep/14.2.363 · DOI
10.1093/oxfordjournals.humrep.a019139 · DOI
10.1046/j.1365-2605.1999.00162.x · DOI
10.1111/j.1439-0272.1998.tb01379.x · DOI
10.1016/S0015-0282(00)01740-4 · DOI
10.1093/oxfordjournals.humrep.a137472 · DOI
10.1016/S0015-0282(16)58581-1 · DOI
10.1034/j.1600-0412.1999.780710.x · DOI
10.1034/j.1600-0412.1999.780412.x · DOI
10.1034/j.1600-0412.2000.079003189.x · DOI
10.1034/j.1600-0412.2000.079007559.x · DOI
10.1093/molehr/2.8.613 · DOI
10.1093/humrep/13.5.1240 · DOI
10.1023/A:1009406231811 · DOI
10.1002/j.1939-4640.2000.tb03273.x · DOI
10.3109/01485019008987614 · DOI
10.1002/j.1939-4640.2001.tb02198.x · DOI
10.1093/humrep/14.8.2015 · DOI
10.1093/humrep/15.8.1717 · DOI
10.1016/S0015-0282(16)55566-6 · DOI
10.1111/j.1365-2605.1996.tb00429.x · DOI
10.1093/humrep/13.4.896 · DOI
10.1016/S0015-0282(97)00536-0 · DOI
10.1089/dna.1997.16.1087 · DOI
10.1095/biolreprod52.4.864 · DOI
10.1016/0027-5107(88)90048-6 · DOI
10.1002/(SICI)1098-2795(200006)56:2 <235::AID-MRD4>3.0.CO;2-D · DOI
10.1016/S0027-5107(97)00005-5 · DOI
10.1006/dbio.1993.1173 · DOI
Morse‐Gaudio M., 1994, Topoisomerase II expression and VM‐26 induction of DNA breaks during spermatogenesis in Xenopus laevis, J Cell Sci., 107, 2887, 10.1242/jcs.107.10.2887 · DOI
10.1093/oxfordjournals.humrep.a137300 · DOI
10.1093/humrep/15.suppl_5.98 · DOI
10.1002/j.1939-4640.2000.tb02116.x · DOI
10.1016/S0015-0282(16)53709-1 · DOI
10.1007/978-3-662-05739-1_1 · DOI
10.1093/humupd/5.5.399 · DOI
10.1093/humrep/16.9.1912 · DOI · Paper page
10.1016/S0015-0282(00)00623-3 · DOI
10.1016/S0015-0282(16)54918-8 · DOI
10.1152/ajpendo.1999.276.2.E310 · DOI
Perreault SD, 2001, Teplice Program: Impact of Air Pollution on Human Health, 145
10.1016/S0027-5107(98)00242-5 · DOI
10.1007/BF00288496 · DOI
10.1002/j.1460-2075.1989.tb03581.x · DOI
10.1093/emboj/16.9.2262 · DOI
10.1093/oxfordjournals.humrep.a019071 · DOI
10.1002/j.1939-4640.1995.tb01735.x · DOI
10.1002/em.2850250105 · DOI
10.1093/humrep/15.5.1112 · DOI
10.1530/ror.0.0040031 · DOI
10.1006/excr.1999.4586 · DOI
10.1055/s-2000-12552 · DOI
10.1093/oxfordjournals.humrep.a019263 · DOI
10.1093/oxfordjournals.humrep.a138444 · DOI
10.1016/0890-6238(88)90020-2 · DOI
Selevan SG, 2000, Semen quality and reproductive health of young Czech men exposed to seasonal air pollution, Environ Health Perspect., 108, 1, 10.1289/ehp.00108887 · DOI
10.1530/jrf.0.1010435 · DOI
10.1002/mrd.1080290310 · DOI
10.1016/0014-4827(89)90344-3 · DOI
10.1016/0014-4827(88)90265-0 · DOI · Paper page
10.1093/mutage/13.1.75 · DOI
10.1210/en.136.6.2770 · DOI
10.1016/S0015-0282(99)00462-8 · DOI
10.1093/humrep/13.9.2495 · DOI
10.1095/biolreprod56.3.602 · DOI
10.1016/S0015-0282(16)47963-X · DOI
10.1007/978-94-009-7675-7_46 · DOI
10.1093/humrep/13.6.1429 · DOI
10.1093/humrep/13.7.1864 · DOI
10.1093/molehr/4.5.439 · DOI
10.1016/0301-2115(96)02467-0 · DOI
10.1095/biolreprod44.4.569 · DOI
10.1615/CritRevEukarGeneExpr.v6.i2-3.30 · DOI
World Health Organization, 1993, WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Couple
Yanagimachi R., 1995, Is an animal model needed for intracytoplasmic sperm infection (ICSI) and other assisted reproduction technologies?, Hum Reprod., 10, 2525
10.1093/HUMREP/10.6.1444 · DOI

Sorted by citations

Items 301–400 of 906

Simultaneous determination in situ of DNA fragmentation and 8-oxoguanine in human sperm.
Santiso, R., Tamayo, M., Gosálvez, J., Meseguer, M., Garrido, N., & Fernández, J. (2010) · Fertility and Sterility · doi:10.1016/j.fertnstert.2009.07.969 · Cited by 53
Relationships between climate and sperm quality in dairy bull semen: A retrospective analysis.
Sabés-Alsina, M., Lundeheim, N., Johannisson, A., López-Béjar, M., & Morrell, J. (2019) · Journal of Dairy Science · doi:10.3168/jds.2018-15837 · Cited by 52
Sperm DNA fragmentation index does not correlate with blastocyst aneuploidy or morphological grading
Gat, I., Tang, K., Quach, K., Kuznyetsov, V., Antes, R., Filice, M., Zohni, K., & Librach, C. L. (2017) · PLoS ONE · doi:10.1371/journal.pone.0179002 · Cited by 52
Red light improves spermatozoa motility and does not induce oxidative DNA damage
Preece, D., Chow, K. W., Gomez‐Godinez, V., Gustafson, K., Esener, S., Ravida, N., Durrant, B., & Berns, M. W. (2017) · Scientific Reports · doi:10.1038/srep46480 · Cited by 49
Viable offspring obtained from Prm1-deficient sperm in mice
Takeda, N., Yoshinaga, K., Furushima, K., Takamune, K., Li, Z.-H., Abe, S., Aizawa, S., & Yamamura, K. (2016) · Scientific Reports · doi:10.1038/srep27409 · Cited by 52
“Both the comet assay and SCSA are thought to detect sperm DNA damage or integrity 22,35 , and DNA damage is considered to be the cause of infertility in Prm1 + / − mice 5,6 .”
Influential
Correlation between sperm DNA fragmentation index and CMA3 positive spermatozoa in globozoospermic patients
Hosseinifar, H., Yazdanikhah, S., Modarresi, T., Totonchi, M., Gilani, M. A. S., & Sabbaghian, M. (2015) · Andrology · doi:10.1111/andr.12030 · Cited by 55
“The percentage of abnormal spermatozoa with detectable DFI (%DFI) was calculated from the DFI frequency dot plot obtained from the ratio between the red and total (red plus green) fluorescence intensity (Evenson et al., 2002) (Fig.”
Method
Chromatin fluorescence characteristics and standard semen analysis parameters: correlations observed in andrology testing among 136 males referred for infertility evaluation
Sills, E. S., Fryman, J. T., Perloe, M., Michels, K., & Tucker, M. (2004) · Journal of Obstetrics and Gynaecology Research · doi:10.1080/01443610310001620369 · Cited by 52
“From these observations, it has been suggested that elevated sperm DNA fragmentation or inappropriately high DNA stainability may be considered evidence for less competent spermatozoa (Evenson et al., 2002).”
Background
Normospermic Versus Teratospermic Domestic Cat Sperm Chromatin Integrity Evaluated by Flow Cytometry and Intracytoplasmic Sperm Injection1
Penfold, L., Jost, L., Evenson, D., & Wildt, D. (2003) · Biology of Reproduction · doi:10.1095/biolreprod.103.016089 · Cited by 52
Self-citation
The role of sperm DNA fragmentation testing in predicting intra-uterine insemination outcome: A systematic review and meta-analysis.
Sugihara, A., Avermaete, F. V., Roelant, E., Punjabi, U., & Neubourg, D. D. (2019) · European Journal of Obstetrics, Gynecology, and Reproductive Biology · doi:10.1016/j.ejogrb.2019.10.005 · Cited by 51
Developmental exposure to decabrominated diphenyl ether (BDE‐209): Effects on sperm oxidative stress and chromatin dna damage in mouse offspring
Tseng, L., Hsu, P., Lee, C.-W., Tsai, S., Pan, M.-H., & Li, M.-H. (2013) · Environmental Toxicology · doi:10.1002/tox.20729 · Cited by 51
“The SCSA described by Evenson et al. (2002) is used to detect the susceptibility of sperm to in situ acid denaturation of DNA.”
Method
Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians
Farkouh, A., Salvio, G., Kuroda, S., Saleh, R., Vogiatzi, P., & Agarwal, A. (2022) · Translational Andrology and Urology · doi:10.21037/tau-22-149 · Cited by 50
“Moreover, this method also reports % high DNA stainability (HDS) which indicates the extent of stainability of the sperm nucleus, representing immature spermatozoa (90).”
Method
Sperm DNA damage and its impact on male reproductive health: a critical review for clinicians, reproductive professionals and researchers
Agarwal, A. K., Selvam, M. K. P., Baskaran, S., & Cho, C. (2019) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2019.1614916 · Cited by 57
“was established as the clinical reference value of sperm DNA damage using SCSA [86,87].”
MethodInfluential
What should be done for men with sperm DNA fragmentation?
Kim, G. (2018) · Clinical and Experimental Reproductive Medicine · doi:10.5653/cerm.2018.45.3.101 · Cited by 50
“The most important parameters are the DNA fragmentation index (DFI, %), which indicates the number of cells with DNA damage [21], and HDS (%), which indicates the proportion of immature sperm with defects in the histone-to-protamine transition [11].”
Background
Turning the corner in fertility: high DNA integrity of boundary-following sperm.
Eamer, L., Vollmer, M., Nosrati, R., San-Gabriel, M., Zeidan, K., Zini, A., & Sinton, D. (2016) · Lab on a Chip · doi:10.1039/c6lc00490c · Cited by 50
Urinary concentrations of benzophenone-type ultraviolet light filters and semen quality
Louis, G. M. B., Chen, Z., Kim, S., Sapra, K. J., Bae, J., & Kannan, K. (2015) · Fertility and Sterility · doi:10.1016/j.fertnstert.2015.07.1129 · Cited by 52
“The SCSA® was used according to the methods of Evenson (28) to quantify DNA fragmentation and the percent of high stainable sperm.”
Method
Relationship of sperm DNA fragmentation, apoptosis and dysfunction of mitochondrial membrane potential with semen parameters and ART outcome after intracytoplasmic sperm injection
Sharbatoghli, M., Valojerdi, M. R., Amanlou, M., Khosravi, F., & Jafar-abadi, M. A. (2012) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-012-2440-1 · Cited by 50
“The percentage of DF was lower in infertile men who initiated a clinical pregnancy after assisted conception [10–12] than in those who did not [13–15].”
Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing.
Rota, A., Rota, A., Martini, M., Milani, C., & Romagnoli, S. (2005) · Reproduction, nutrition, development · doi:10.1051/rnd:2005002 · Cited by 50
Effect of male age on semen quality in domestic animals: potential for advanced functional and translational research?
Abah, K. O., Fontbonne, A., Partyka, A., & Niżański, W. (2023) · Veterinary research communications · doi:10.1007/s11259-023-10159-1 · Cited by 49
“For example, it is 30% in humans (Evenson et al. 2002), 28% in stallions, and 6% in pigs (Kumaresan et al.”
Background
Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients
Ferrigno, A., Ruvolo, G., Capra, G., Serra, N., & Bosco, L. (2021) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-021-02080-w · Cited by 52
Sperm DNA fragmentation: causes and identification
Hamilton, T. R. D. S., & Assumpção, M. (2019) · Zygote (Cambridge. Print) · doi:10.1017/s0967199419000595 · Cited by 49
“In regions with DNA fragmentation there is possible alteration of genetic information (Evenson et al., 2002b).”
BackgroundMethodInfluential
RETRACTED: Moderate aerobic exercise training for improving reproductive function in infertile patients: A randomized controlled trial
Maleki, B. H., & Tartibian, B. (2017) · Cytokine · doi:10.1016/j.cyto.2017.01.007 · Cited by 52
Retracted
Sperm chromatin structural integrity in normospermic boars is not related to semen storage and fertility after routine AI.
Waberski, D., Schapmann, E., Henning, H., Riesenbeck, A., & Brandt, H. (2011) · Theriogenology · doi:10.1016/j.theriogenology.2010.09.004 · Cited by 49
The influence of cryopreservation and seminal plasma on the chromatin structure of dog spermatozoa.
Koderle, M., Aurich, C., & Schäfer-Somi, S. (2009) · Theriogenology · doi:10.1016/j.theriogenology.2009.07.015 · Cited by 49
“High chromatin denaturation is related to poor embryonic development [8,9,15].”
Background
Sperm DNA fragmentation in subfertile men: the effect on the outcome of intracytoplasmic sperm injection and correlation with sperm variables
Nicopoullos, J. D. M., Gilling-Smith, C., Almeida, P., Homa, S., Norman-Taylor, J., & Ramsay, J. W. A. (2008) · BJU International · doi:10.1111/j.1464-410x.2008.07518.x · Cited by 49
Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?
Gosálvez, J., Caballero, P., López-Fernández, C., Ortega, L., Guijarro, J. A., Fernández, J., Johnston, S., & Nuñez-Calonge, R. (2013) · Asian Journal of Andrology · doi:10.1038/aja.2013.74 · Cited by 49
The impact of Chlamydia trachomatis infection on sperm parameters and male fertility: A comprehensive study
Moazenchi, M., Totonchi, M., Yazdi, R. S., Hratian, K., Meybodi, M., Panah, M. A., Chehrazi, M., & Meybodi, A. (2018) · International Journal of STD and AIDS · doi:10.1177/0956462417735245 · Cited by 48
Testicular cancer and sperm DNA damage: short‐ and long‐term effects of antineoplastic treatment
Paoli, D., Gallo, M., Rizzo, F., Spanò, M., Leter, G., Lombardo, F., Lenzi, A., & Gandini, L. (2015) · Andrology · doi:10.1111/j.2047-2927.2014.00250.x · Cited by 48
The determination of reproductive safety in men during and after cancer treatment.
Choy, J., & Brannigan, R. (2013) · Fertility and Sterility · doi:10.1016/j.fertnstert.2013.07.1974 · Cited by 48
“Multiple laboratory techniques are available for the evaluation of sperm DNA integrity, including the sperm chromatin structure assay (SCSA), which measures the extent of DNA denaturability in the presence of an acid-detergent resulting from the presence of accumulated strand breaks (22); the terminal deoxynucleotidyl…”
Method
Function of the Sperm Nuclear Matrix
Shaman, J. A., Yamauchi, Y., & Ward, W. (2007) · Archives of andrology · doi:10.1080/01485010701329378 · Cited by 48
“On the one hand, we predicted, based on chromatin structure, that both the Sperm Chromatin Structure Assay (SCSA) [Evenson et al. 2002] and the TUNEL assay [Spano et al. 2005] detect non-toroidal single and double stranded breaks.”
Influential
The importance of the one carbon cycle nutritional support in human male fertility: a preliminary clinical report
Dattilo, M., Cornet, D., Amar, E., Cohen, M., & Ménézo, Y. (2014) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-12-71 · Cited by 47
“However this is unlikely as, in the absence of any therapeutic intervention, DFI and SDI are much more stable over time than the classical semen parameters [24-26].”
Background
Quality of bull spermatozoa after preparation by single-layer centrifugation.
Goodla, L., Morrell, J., Yusnizar, Y., Stålhammar, H., & Johannisson, A. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7607 · Cited by 47
Amifostine-doxorubicin association causes long-term prepubertal spermatogonia DNA damage and early developmental arrest.
Vendramini, V., Robaire, B., & Miraglia, S. M. (2012) · Human Reproduction · doi:10.1093/humrep/des159 · Cited by 47
“This analysis was done according to the previously described assay (Evenson et al., 2002; Delbès et al., 2007) that was developed to quantify the susceptibility of sperm DNA to denaturation when exposed to low pH treatment and stained with acridine orange (AO).”
Method
Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one?
Nadalini, M., Tarozzi, N., Santo, M. D., & Borini, A. (2011) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-014-0267-3 · Cited by 47
Cytogenetic, Y chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions
Venkatesh, S., Thilagavathi, J., Kumar, K., Deka, D., Talwar, P., & Dada, R. (2011) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-011-1990-y · Cited by 47
“cally accepted in the evaluation of male infertility and spontaneous abortion [23, 30].”
Background
DNA Status on Thawed Semen from Fighting Bull: A Comparison Between the SCD and the SCSA Tests
Martínez‐Pastor, F., Fernández-Santos, M. R., Domínguez‐Rebolledo, Á., Esteso, M., Garde, J. J., & Biology of Reproduction Group (2008) · Reproduction in Domestic Animals · doi:10.1111/j.1439-0531.2008.01098.x · Cited by 52
Prognostic markers for competent human spermatozoa: fertilizing capacity and contribution to the embryo
Steger, K., Cavalcanti, M. C. O., & Schuppe, H. (2010) · International Journal of Andrology · doi:10.1111/j.1365-2605.2010.01129.x · Cited by 58
“spermatozoa in a semen sample may suggest use of ICSI for maximizing pregnancy rates (Evenson et al., 2002; Evenson & Wixon, 2006; Bungum et al., 2007).”
Background
Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sperm morphology and chromatin status
Sousa, A. P., Tavares, R. S., de la Calle, J. F. V., Figueiredo, H., Almeida, V., Almeida‐Santos, T., & Ramalho‐Santos, J. (2008) · Human Reproduction · doi:10.1093/humrep/den365 · Cited by 42
“…29200 Brest, France 4Laboratorio FIV, Centro Hospitalar V.N. Gaia, 4400-129 V.N. Gaia, Portugal 5Department of Zoology and Anthropology, Faculty of Sciences, University of Oporto, and Centro de Estudos de Infertilidade e Esterilidade (CEIE), 4050-345 Oporto, Portugal 6Correspondence address.”
Influential
Critically short telomeres are associated with sperm DNA fragmentation.
Rodriguez, S., Goyanes, V., Segrelles, E., Blasco, M., Gosálvez, J., & Fernández, J. (2005) · Fertility and Sterility · doi:10.1016/j.fertnstert.2005.05.014 · Cited by 46
Clinical Laboratory Evaluation of Male Subfertility
Weber, R. F. A., Dohle, G. R., & Romijn, J. C. (2005) · Advances in clinical chemistry · doi:10.1016/s0065-2423(05)40008-6 · Cited by 46
Sperm DNA fragmentation index with unexplained recurrent spontaneous abortion: A systematic review and meta-analysis.
Pu, Y.-F., Yan, L., Lu, S.-M., Guo, Y.-J., & Zhu, X.-Y. (2020) · Journal of Gynecology Obstetrics and Human Reproduction · doi:10.1016/j.jogoh.2020.101740 · Cited by 45
Combined effects of resveratrol and epigallocatechin-3-gallate on post thaw boar sperm and IVF parameters.
Bucci, D., Spinaci, M., Yeste, M., Mislei, B., Gadani, B., Prieto-Martínez, N., Love, C., Mari, G., Tamanini, C., & Galeati, G. (2018) · Theriogenology · doi:10.1016/j.theriogenology.2018.05.016 · Cited by 45
Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment
Evenson, D. (2017) · Translational Andrology and Urology · doi:10.21037/tau.2017.07.20 · Cited by 45
“Data are presented as a dot plot (one dot for each of 5,000 sperm) with both X and Y axes of 1,024 channels or 1,024 degrees of DNA damage (14) as seen in Figure 1.”
BackgroundMethodSelf-citation
Semen quality and pregnancy loss in a contemporary cohort of couples recruited before conception: data from the Longitudinal Investigation of Fertility and the Environment (LIFE) Study
Eisenberg, M. L., Sapra, K. J., Kim, S. D., Chen, Z., & Louis, G. M. B. (2017) · Fertility and Sterility · doi:10.1016/j.fertnstert.2017.07.008 · Cited by 47
The predictive value of sperm chromatin structure assay.
Nicopoullos, J. D. M., Gilling-Smith, C., Almeida, P., & Ramsay, J. (2005) · Human Reproduction · doi:10.1093/humrep/deh630 · Cited by 45
Sperm Dna Integrity: Correlation With Sperm Plasma Membrane Integrity In Semen Evaluated For Male Infertility
Moskovtsev, S., Willis, J., Azad, A., & Mullen, J. (2005) · Archives of andrology · doi:10.1080/014850190512770 · Cited by 45
“However, others have reported no correlation between DNA integrity and standard semen parameters [23, 24, 38, 39].”
Background
Cytoplasmic transfer in oocytes: biochemical aspects.
Lévy, R., Elder, K., & Menezo, Y. (2004) · Human Reproduction Update · doi:10.1093/humupd/dmh016 · Cited by 45
“The marked effect of sperm DNA fragmentation on embryo developmental potential and unexplained recurrent pregnancy loss is now well documented (Evenson et al., 2002; Carrell et al., 2003).”
Sperm DNA damage output parameters measured by the alkaline Comet assay and their importance
Simon, L., Aston, K., Emery, B., Hotaling, J. M., & Carrell, D. (2017) · Andrologia · doi:10.1111/and.12608 · Cited by 44
“To measure sperm DNA damage, four assays are commonly used, namely sperm chromatin structure assay (SCSA; Evenson et al., 1980, 2002), terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL; Gorczyca et al., 1993), sperm chromatin dispersion (SCD) assay (Fernandez et al., 2003) and…”
Method
Relationship between chromatin condensation, DNA integrity and quality of ejaculated spermatozoa from infertile men
Manochantr, S., Chiamchanya, C., & Sobhon, P. (2012) · Andrologia · doi:10.1111/j.1439-0272.2010.01128.x · Cited by 44
“These parameters were completely interrelated and have been deserted as a reflection of damage of sperm chromatin during spermiogenesis and subsequent maturation (Spano et al., 1998; Evenson et al., 2002).”
Background
Dietary exposure to methyl mercury and PCB and the associations with semen parameters among Swedish fishermen
Rignell-Hydbom, A., Axmon, A., Lundh, T., Jönsson, B., Tiido, T., & Spanô, M. (2007) · Environmental Health · doi:10.1186/1476-069x-6-14 · Cited by 44
“Age and abstinence time [33,37-42], BMI [43], season of sampling [44-48], and previous reproductive malformations and diseases [49,50] have all previously been found to be associated with different measures of male reproduction and where thus considered as potential confounders.”
BackgroundMethod
Seasonal changes in sperm chromatin condensation in ram (Ovis aries), Iberian red deer (Cervus elaphus hispanicus), and brown bear (Ursus arctos).
García-Macías, V., Martínez-Pastor, F., Alvarez, M., Borragán, S., Chamorro, C., Soler, A., Anel, L., & de Paz, P. (2006) · Journal of Andrology · doi:10.2164/jandrol.106.000315 · Cited by 44
Sperm DNA fragmentation assay by sperm chromatin dispersion (SCD): correlation between DNA fragmentation and outcome of intracytoplasmic sperm injection
Sivanarayana, T., Krishna, C. R., Prakash, G. J., Krishna, K. M., Madan, K., Sudhakar, G., & Gottumukkala, R. R. A. (2014) · Reproductive Medicine and Biology · doi:10.1007/s12522-013-0168-7 · Cited by 43
“It has been suggested by some studies that when spermatozoa shows more than 30 % DNA fragmentation natural conception is compromised [7].”
Background
Single layer centrifugation (SLC) improves sperm quality of cryopreserved Blanca-Celtibérica buck semen.
Jiménez-Rabadán, P., Morrell, J., Johannisson, A., Ramón, M., García-Álvarez, O., Maroto-Morales, A., Alvaro-García, P. J., Pérez-guzmán, M., Fernández-Santos, M., Garde, J. J., et al. (2012) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2012.09.012 · Cited by 43
Evidence that single-stranded DNA breaks are a normal feature of koala sperm chromatin, while double-stranded DNA breaks are indicative of DNA damage.
Zee, Y., López-Fernández, C., Arroyo, F., Johnston, S., Holt, W., & Gosálvez, J. (2009) · Reproduction · doi:10.1530/rep-09-0021 · Cited by 43
“…02:31:28PM via free access demonstrated as a highly predictive indicator of male factor infertility (Agarwal & Said 2003), particularly when accounting for early embryonic loss, which cannot always be explained by traditional parameters of sperm quality (Zini et al. 2001, Evenson et al. 2002).”
Background
Analysis of telomere length in couples experiencing idiopathic recurrent pregnancy loss
Thilagavathi, J., Mishra, S., KUMAR, M., Vemprala, K., Deka, D., Dhadwal, V., & Dada, R. (2013) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-013-9993-1 · Cited by 43
“Critically short telomeres are associated with sperm DNA fragmentation in mice and abnormal embryonic development in man [12, 13].”
Background
Cytometric solutions in veterinary andrology: Developments, advantages, and limitations
Petrunkina, A., & Harrison, R. (2011) · Cytometry Part A · doi:10.1002/cyto.a.21044 · Cited by 42
“However, it expresses different fluorescence properties depending on whether the DNA is single- or double-stranded: red in the former case and green in the latter (67,68).”
Background
Effect of antioxidants on sperm genetic damage.
Menezo, Y., Evenson, D., Cohen, M., & Dale, B. (2014) · Advances in Experimental Medicine and Biology · doi:10.1007/978-1-4614-7783-9_11 · Cited by 42
Self-citation
Paternal age and sperm DNA decay: discrepancy between chromomycin and aniline blue staining.
Belloc, S., Benkhalifa, M., Junca, A., Dumont, M., Bacrie, P. C., & Ménézo, Y. (2009) · Reproductive Biomedicine Online · doi:10.1016/s1472-6483(10)60083-1 · Cited by 42
Sperm chromatin assessment
Agarwal, A., Tsarev, I., Ērenpreiss, J., & Sharma, R. (2017) · doi:10.1201/9781351228220-6 · Cited by 41
“In addition, a DFI threshold was established that identifies samples compatible with pregnancy (< 30%).(6) To the best of our knowledge, SCSA is the most successful assay in predicting the various outcomes of ART including the fertilization and implantation rates.”
Background
Air Pollution and Health Effects
Nadadur, S. S., & Hollingsworth, J. (2015) · Molecular and Integrative Toxicology · doi:10.1007/978-1-4471-6669-6 · Cited by 41
Sperm chromatin structure components are differentially repaired in cancer survivors.
O'Flaherty, C., Chan, P., Hales, B., & Robaire, B. (2012) · Journal of Andrology · doi:10.2164/jandrol.111.015388 · Cited by 41
“To assess the susceptibility of sperm DNA to acid-induced denaturation, SCSA analysis was done as described previously (Evenson et al, 2002).”
Method
Refrigerated storage of red deer epididymal spermatozoa in the epididymis, diluted and with vitamin C supplementation.
Fernández-Santos, M., Domínguez-Rebolledo, Á., Esteso, M., Garde, J., & Martínez-Pastor, F. (2009) · Reproduction in domestic animals = Zuchthygiene · doi:10.1111/j.1439-0531.2007.01032.x · Cited by 41
“Increased DFI has been associated with increased risk of miscarriage and increased time to pregnancy in humans (Larson et al. 2000; Evenson et al. 2002) and bulls and mice (Evenson et al.”
Background
Infection du tractus génital masculin : le point de vue du bactériologiste
Askienazy-Elbhar, M. (2005) · doi:10.1016/j.gyobfe.2005.07.008 · Cited by 41
Easy sperm processing technique allowing exclusive accumulation and later usage of DNA-strandbreak-free spermatozoa.
Ebner, T., Shebl, O., Moser, M., Mayer, R., Arzt, W., & Tews, G. (2011) · Reproductive Biomedicine Online · doi:10.1016/j.rbmo.2010.09.004 · Cited by 41
“These assays can be subdivided into two categories, those directly detecting DNA damage (e.g. TUNEL) and those measuring DNA fragmentation after a rather mild denaturation process (e.g. SCSA, SCD).”
MethodInfluential
Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection
Jerre, E., Bungum, M., Evenson, D. P., & Giwercman, A. (2019) · Fertility and Sterility · doi:10.1016/j.fertnstert.2019.03.013 · Cited by 54
Self-citation
Carnitine partially improves oxidative stress, acrosome integrity, and reproductive competence in doxorubicin‐treated rats
Cabral, R. E. L., Mendes, T. B., Vendramini, V., & Miraglia, S. M. (2018) · Andrology · doi:10.1111/andr.12426 · Cited by 40
“The analysis of sperm chromatin integrity using acridine orange was performed as described by Evenson et al. (2002) and Vendramini et al. (2012), with minor modifications.”
Method
Methods of Assessment of Cryopreserved Semen
Partyka, A., Niżański, W., & Ochota, M. (2012) · doi:10.5772/33565 · Cited by 40
“It is based on the assumption that a structurally abnormal sperm chromatin shows a higher susceptibility to acid denaturation (Evenson et al., 2002).”
Background
Resveratrol improves sperm DNA quality and reproductive capacity in type 1 diabetes
Simas, J. N., Mendes, T. B., Fischer, L. W., Vendramini, V., & Miraglia, S. M. (2021) · Andrology · doi:10.1111/andr.12891 · Cited by 39
Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep.
Guan, Y.-J., Malecki, I., Hawken, P., Linden, M., & Martin, G. (2014) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2014.07.014 · Cited by 39
“The %DFI in rams is much lower than humans due to well established differences between the density of chromatin packaging between species (Evenson et al., 2002).”
Background
Assessment of different functional parameters of frozen-thawed buffalo spermatozoa by using cytofluorimetric determinations.
Minervini, F., Guastamacchia, R., Pizzi, F., Dell'Aquila, M. E., & Barile, V. (2013) · Reproduction in domestic animals = Zuchthygiene · doi:10.1111/j.1439-0531.2012.02152.x · Cited by 39
“Cryopreservation procedures can impair the DNA integrity, damaging embryonic development with consequent subfertility and infertility of several species (Evenson et al. 2002; Gillan et al. 2005).”
Background
Analysis of sperm nuclear protein gene polymorphisms and DNA integrity in infertile men
Venkatesh, S., Kumar, R., Deka, D., Deecaraman, M., & Dada, R. (2011) · Systems Biology in Reproductive Medicine · doi:10.3109/19396368.2011.562960 · Cited by 39
“SCSA was performed [Evenson et al. 2002] with slight modification.”
Method
Sperm chromatin quality and DNA integrity in partial versus total globozoospermia
Talebi, A., Ghasemzadeh, J., Khalili, M., Halvaei, I., & Fesahat, F. (2018) · Andrologia · doi:10.1111/and.12823 · Cited by 38
The relationship of sperm DNA fragmentation index with the outcomes of in-vitro fertilisation-embryo transfer and intracytoplasmic sperm injection
Jiang, H., He, R.-B., Wang, C., & Zhu, J. (2011) · Journal of Obstetrics and Gynaecology Research · doi:10.3109/01443615.2011.590910 · Cited by 38
“In humans, it has been demonstrated that men possess high fecundity with sperm DFI value in 0 – 15; moderate fecundity with DFI 16 – 29; low fecundity with DFI 30% and incompatible fertility with DFI 80% (Evenson et al. 2002).”
Background
Ram semen evaluation: Development and efficiency of modern techniques
Tsakmakidis, I. (2010) · doi:10.1016/j.smallrumres.2010.04.017 · Cited by 38
“This is associated with disturbances during spermatogenesis or to subsequent chromatin damage and leads to reduced fertility (Evenson et al., 2002).”
Background
Sperm DNA Integrity in Men Treated for Childhood Cancer
Romerius, P., Ståhl, O., Moëll, C., Relander, T., Cavallin-ståhl, E., Gustafsson, H., Thapper, K. L., Jepson, K., Spanô, M., Wiebe, T., et al. (2010) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-0140 · Cited by 38
“DFI hereby expresses the proportion of cells containing denatured DNA (17).”
Background
Utility and Predictive Value of Human Standard Semen Parameters and Sperm DNA Dispersion for Fertility Potential
Gill, K., Jakubik, J., Rosiak-Gill, A., Kupś, M., Lukaszuk, M., Kurpisz, M., Fraczek, M., & Piasecka, M. (2019) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph16112004 · Cited by 37
Fluorescence technologies for evaluating male gamete (dys)function.
Petrunkina, A., & Harrison, R. (2013) · Reproduction in domestic animals = Zuchthygiene · doi:10.1111/rda.12202 · Cited by 37
“In the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, sperm are fixed and denatured to unravel the nuclear DNA and allow access of the transferase enzyme mediating attachment of a fluorochrome-conjugated deoxyribonucleotide to the 3′-hydroxyl ends of all free breaks in the DNA strands (Su…”
MethodInfluential
The use of complimentary assays to evaluate the enrichment of human sperm quality in asthenoteratozoospermic and teratozoospermic samples processed with Annexin‐V magnetic activated cell sorting
Delbes, G., Herrero, M. B., Troeung, E.-T., & Chan, P. T. (2013) · Andrology · doi:10.1111/j.2047-2927.2013.00106.x · Cited by 37
“Sperm chromatin structure assay To measure the susceptibility of sperm nuclear DNA to low pH induced denaturation in situ, the acridine orange (AO) assay was applied, using the method previously described as the SCSA (Evenson et al., 2002).”
Method
Disruption of Telomere–Telomere Interactions Associated with DNA Damage in Human Spermatozoa
Moskovtsev, S. I., Willis, J., White, J., & Mullen, J. B. M. (2010) · Systems Biology in Reproductive Medicine · doi:10.3109/19396368.2010.502587 · Cited by 38
“The differences in the levels of DNA damage in spermatozoa among fertile and infertile men suggests that sperm DNA damage could be used as a predictor of fertility potential [Evenson et al. 2002; Spano et al. 2000].”
BackgroundMethod
Effects of long-term chilled storage of red deer epididymides on DNA integrity and motility of thawed spermatozoa.
Fernández-Santos, M., Martínez-Pastor, F., Matias, D., Domínguez-Rebolledo, Á., Esteso, M., Montoro, V., & Garde, J. (2009) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2008.02.001 · Cited by 37
“The SCSA technique has many major advantages over other assays, which are often labour intensive and lack statistical power for diagnosis and prognosis (Evenson et al., 2002).”
Method
Sperm DNA fragmentation in a random sample of the Spanish boar livestock.
López-Fernández, C., Pérez-Llano, B., García-Casado, P., Sala, R., Gosálbez, A., Arroyo, F., Fernández, J., & Gosálvez, J. (2008) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2006.11.015 · Cited by 37
Post-thaw viability of bull AI-doses with low-sperm numbers.
Ballester, J., Johannisson, A., Saravia, F., Håård, M., Gustafsson, H., Bajramovic, D., & Rodríguez-Martínez, H. (2007) · Theriogenology · doi:10.1016/j.theriogenology.2007.07.008 · Cited by 37
“This shift is expressed as the DNA fragmentation index (DFI), representing the cells with an excess of ssDNA, the standard deviation of the DFI (SD-DFI) and the mean of the amount of denatured ssDNA over the total cellular DNA, calculated for each spermatozoon in a sample (xDFI [34]).”
BackgroundMethod
Sperm DNA Fragmentation and Male Infertility
Selvam, M. K. P., Sengupta, P., & Agarwal, A. K. (2020) · Springer eBooks · doi:10.1007/978-3-030-37972-8_9 · Cited by 47
Ethanol-induced male infertility: Effects of aqueous leaf extract of Tetracarpidium conophorum
Akomolafe, S. F., Oboh, G., Akindahunsi, A. A., & Afolayan, A. J. (2017) · Andrologia · doi:10.1111/and.12759 · Cited by 33
Semen analysis: update on clinical value, current needs and future perspectives
Sánchez, V., Wistuba, J., & Mallidis, C. (2013) · Reproduction · doi:10.1530/rep-13-0109 · Cited by 39
“pdf – accessed 13th February 2013) was derived using SCSA (Evenson et al. 2002).”
MethodInfluential
Spermatozoa DNA damage measured by sperm chromatin structure assay (SCSA) and birth characteristics in children conceived by IVF and ICSI.
Bungum, M., Bungum, L., Lynch, K., Wedlund, L., Humaidan, P., & Giwercman, A. (2012) · International Journal of Andrology · doi:10.1111/j.1365-2605.2011.01222.x · Cited by 36
The usefulness of combining traditional sperm assessments with in vitro heterospermic insemination to identify bulls of low fertility as estimated in vivo.
Puglisi, R., Pozzi, A., Foglio, L., Spanô, M., Eleuteri, P., Grollino, M., Bongioni, G., & Galli, A. (2012) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2012.04.006 · Cited by 36
High Levels of DNA Fragmentation in Spermatozoa Are Associated with Inbreeding and Poor Sperm Quality in Endangered Ungulates1
Ruiz-López, M., Evenson, D., Espeso, G., Gomendio, M., & Roldan, E. R. (2010) · Biology of Reproduction · doi:10.1095/biolreprod.110.084798 · Cited by 36
Self-citation
Sperm chromatin integrity in young men with no experiences of infertility and men from idiopathic infertility couples
Rybar, R., Marková, P., Vĕzník, Z., Faldíková, L., Kunetková, M., Zajícová, A., Kopecká, V., & Rubeš, J. (2009) · Andrologia · doi:10.1111/j.1439-0272.2008.00905.x · Cited by 35
“Chromatin integrity is sometimes described as a much more objective marker of sperm function than standard semen analysis due to the fact that the latter fails to evaluate objectively the functional integrity of the highly specialised sperm cells (Evenson et al., 1991, 2002; Zini et al., 2001b).”
BackgroundMethod
Factors affecting chromatin stability of bovine spermatozoa.
Khalifa, T., Rekkas, C., Lymberopoulos, A., Sioga, A., Dimitriadis, I., & Papanikolaou, T. (2008) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2007.02.019 · Cited by 36
“Consequently, the biological effect of abnormal sperm chromatin structure depends on the combined effects of the level of chromatin damage in the spermatozoa and the capacity of the oocyte to repair that pre-existing damage (Evenson et al., 2002).”
Background
Male Urinary Biomarkers of Antimicrobial Exposure and Bi-directional Associations with Semen Quality Parameters
Smarr, M., Honda, M., Kannan, K., Chen, Z., Kim, S., & Louis, G. B. B. (2018) · Reproductive Toxicology · doi:10.1016/j.reprotox.2018.02.008 · Cited by 35
“Moreover, none of the urinary antimicrobials were associated with sperm DNA integrity, as measured by the percentage of DNA fragmentation or high stainability [32, 38].”
BackgroundMethodInfluential
Seminal parameters of chronic male genital inflammation are associated with disturbed sperm DNA integrity
Haidl, F., Haidl, G., Oltermann, I., & Allam, J. (2015) · Andrologia · doi:10.1111/and.12408 · Cited by 35
Sperm cryopreservation in brown bear (Ursus arctos): preliminary aspects.
Anel, L., Alvarez, M., Martínez-Pastor, F., Gomes, S., Nicolas, M., Mata, M., Martínez, A. F., Borragán, S., Anel, E., & de Paz, P. (2008) · Reproduction in domestic animals = Zuchthygiene · doi:10.1111/j.1439-0531.2008.01248.x · Cited by 35
“Chromatin status analysis is a critical protocol that provides interesting data on the quality of the sperm cell (Evenson et al. 2002) and which we have tested on bear spermatozoa (Garcia-Macias et al. 2006c).”
Background
Correlation among isolated teratozoospermia, sperm DNA fragmentation and markers of systemic inflammation in primary infertile men
Candela, L., Boeri, L., Capogrosso, P., Cazzaniga, W., Pozzi, E., Belladelli, F., Baudo, A., Ravizzoli, A., Ventimiglia, E., Viganò, P., et al. (2021) · PLOS ONE · doi:10.1371/journal.pone.0251608 · Cited by 35
“Sperm DNA fragmentation (SDF) index, as measured by sperm chromatin structure assay (SCSA), was assessed only in infertile men, considering a pathologic SDF threshold of�30% [1, 22, 23].”
Method
Attenuation of cryopreservation-induced oxidative stress by antioxidant: Impact of Coenzyme Q10 on the quality of post-thawed buck spermatozoa.
Yousefian, I., Emamverdi, M., Karamzadeh-Dehaghani, A., Sabzian-Melei, R., Zhandi, M., & Zare-shahneh, A. (2018) · Cryobiology · doi:10.1016/j.cryobiol.2018.02.005 · Cited by 34
Seasonal changes of DNA fragmentation and quality of raw and cold-stored stallion spermatozoa.
Wach-Gygax, L., Burger, D., Malama, E., Bollwein, H., Fleisch, A., Jeannerat, E., Thomas, S., Schuler, G., & Janett, F. (2017) · Theriogenology · doi:10.1016/j.theriogenology.2017.05.025 · Cited by 34
“To describe DFI distribution the mean DFI, variation (SD of DFI) and the percentage of the cells outside the main population (% DFI) were determined [11, 20, 21].”
Method
Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success
Fortunato, A., Boni, R., Leo, R., Nacchia, G., Liguori, F., Casale, S., Bonassisa, P., & Tosti, E. (2015) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2015.10.009 · Cited by 33
“Recently, chromatin decondensation and DNA fragmentation have been claimed as new markers of sperm immaturity and malfunctioning, and it has been suggested that these evaluations are included alongside the conventional semen analysis (Evenson et al., 2002; Lefièvre et al., 2007; Tosti and Fortunato, 2011; Zini, 2011;…”
Background
Characterisation of a subpopulation of sperm with massive nuclear damage, as recognised with the sperm chromatin dispersion test
Gosálvez, J., Rodríguez-Predreira, M., Mosquera, A., López-Fernández, C., Esteves, S. C., Agarwal, A., & Fernández, J. (2014) · Andrologia · doi:10.1111/and.12118 · Cited by 34
“In fact, the presence of fragmented DNA sperm is higher in infertile patients and in subjects with low sperm quality (Evenson et al., 2002; Evenson & Wixon, 2006).”
Background
Membrane phase behavior during cooling of stallion sperm and its correlation with freezability
Oldenhof, H., Friedel, K., Akhoondi, M., Gojowsky, M., Wolkers, W., & Sieme, H. (2012) · Molecular membrane biology · doi:10.3109/09687688.2012.674161 · Cited by 34
The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors.
van Kooij, R. V., de Boer, P., Vreeden-Elbertse, J. M. T. D., Ganga, N., Singh, N., & Velde, E. T. T. (2004) · International Journal of Andrology · doi:10.1111/j.1365-2605.2004.00463.x · Cited by 34
Effects of season on bull sperm quality in thawed samples in northern Spain
Sabés-Alsina, M., Johannisson, A., Lundeheim, N., López-Béjar, M., & Morrell, J. (2017) · The Veterinary Record · doi:10.1136/vr.103897 · Cited by 33

Authors: EVENSON, D. P., LARSON, K. L., & JOST, L. K.
Affiliations: South Dakota State University (US)
Corresponding author: EVENSON, DONALD P.
ORCID: EVENSON, DONALD P.
Venue: Journal of Andrology
Publisher: Wiley
Published: 2002-01-02
Volume: 23
Issue: 1
Pages: 25-43
ISSN: 0196-3635 (ISSN-L)
1939-4640
DOI: 10.1002/j.1939-4640.2002.tb02599.x
OpenAlex: W1532901402
Cited by: 1,072
References: 158
Citations by year: 2026: 15 · 2025: 21 · 2024: 18 · 2023: 25 · 2022: 36 · 2021: 59 · 2020: 35 · 2019: 50 · 2018: 49 · 2017: 51 · 2016: 42 · 2015: 53 · 2014: 53 · 2013: 68 · 2012: 61
FWCI: 38.26
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 4018 d)
Locations: Journal of Andrology (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation (9452894, 9720642)
U.S. Environmental Protection Agency
South Dakota Agricultural Experiment Station
Topics: Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Health Sciences › Medicine › Urology › Male Reproductive Health Studies
Keywords: sperm chromatin structure assay, sperm DNA fragmentation, male infertility, testicular cancer
MeSH terms: Chromatin, Humans, Infertility, Male, Male, Spermatozoa, DNA Fragmentation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Metadata

Record

About these checks