Cited by 255Mesenchymal stem cell researchImmune cells in cancerCardiac Fibrosis and Remodeling

Macrophages modulate the viability and growth of human mesenchymal stem cells

Freytes, Donald O., Kang, Jung W., Marcos‐Campos, Ivan, Vunjak‐Novakovic, Gordana·2013·Journal of Cellular Biochemistry

Abstract

Following myocardial infarction, tissue repair is mediated by the recruitment of monocytes and their subsequent differentiation into macrophages. Recent findings have revealed the dynamic changes in the presence of polarized macrophages with pro‐inflammatory (M1) and anti‐inflammatory (M2) properties during the early (acute) and late (chronic) stages of cardiac ischemia. Mesenchymal stem cells (MSCs) delivered into the injured myocardium as reparative cells are subjected to the effects of polarized macrophages and the inflammatory milieu. The present study investigated how cytokines and polarized macrophages associated with pro‐inflammatory (M1) and anti‐inflammatory (M2) responses affect the survival of MSCs. Human MSCs were studied using an in vitro platform with individual and combined M1 and M2 cytokines: IL‐1β, IL‐6, TNF‐α, and IFN‐γ (for M1), and IL‐10, TGF‐β1, TGF‐β3, and VEGF (for M2). In addition, polarization molecules (M1: LPS and IFN‐γ; M2: IL‐4 and IL‐13) and common chemokines (SDF‐1 and MCP‐1) found during inflammation were also studied. Indirect and direct co‐cultures were conducted using M1 and M2 polarized human THP‐1 monocytes. M2 macrophages and their associated cytokines supported the growth of hMSCs, while M1 macrophages and their associated cytokines inhibited the growth of hMSCs in vitro under certain conditions. These data imply that an anti‐inflammatory (M2) environment is more accommodating to the therapeutic hMSCs than a pro‐inflammatory (M1) environment at specific concentrations. J. Cell. Biochem. 114: 220–229, 2012. © 2012 Wiley Periodicals, Inc.

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Crossref · 10.1002/jcb.24357

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Authors
Freytes, D. O., Kang, J. W., Marcos‐Campos, I., & Vunjak‐Novakovic, G.
Affiliations
Columbia University (US)
Venue
Journal of Cellular Biochemistry
Publisher
Wiley
Published
2012-11-14
Volume
114
Issue
1
Pages
220-229
ISSN
  • 0730-2312 (ISSN-L)
  • 1097-4644
OpenAlex
W2095029191
Cited by
255
References
31
Citations by year
2026: 5 · 2025: 13 · 2024: 14 · 2023: 12 · 2022: 29 · 2021: 21 · 2020: 19 · 2019: 21 · 2018: 22 · 2017: 27 · 2016: 15 · 2015: 20 · 2014: 20 · 2013: 11 · 2012: 4
FWCI
8.38
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Cellular Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (2r01hl076485-16)
  • National Heart, Lung, and Blood Institute (HL108668, R21HL108668, R01-HL076485, HL076485)
  • National Institute of Biomedical Imaging and Bioengineering (EB-002520, P41EB002520)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
Keywords
mesenchymal stem cells, macrophage polarization, M1 macrophages, M2 macrophages, myocardial infarction, cardiac regeneration, cytokines, IL-10, TGF-β1, vascular endothelial growth factor, chemokines, CXCL12, MCP-1, THP-1 cells, cell viability, cell growth, inflammation
MeSH terms
Cell Communication, Cell Line, Cell Survival, Humans, Inflammation, Lipopolysaccharides, Macrophages, Models, Biological, Cytokines, Coculture Techniques, Vascular Endothelial Growth Factor A, Cell Proliferation, Mesenchymal Stem Cells

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