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Chondroitin sulfate proteoglycan surrounds a subset of human and rat CNS neurons

Bertolotto, Antonio, Rocca, G., Canavese, G., Migheli, A., Schiffer, D.·1991·Journal of Neuroscience Research

Abstract

Chondroitin sulfate proteoglycan (CS-PG) bearing glycosaminoglycan (GAG) chains containing unsulfate (COS) and 6-sulfate (C6S) disaccharides was immunolocalized in rat and human CNS by using monoclonal antibodies (MAb) specific for the two disaccharides. The immunostaining with both MAb was restricted to the periphery of a neuronal subset in rat and human CNS. Double immunofluorescence showed codistribution of the antigens around the same neuronal population. The staining with anti-COS MAb was stronger than with anti-C6S MAb, suggesting that the proteoglycan (PG) contains mainly COS disaccharides. In different rat cortical areas, 40-60/mm2 positive interneurons were found, the visual cortex showing the highest value. In human cortex, positivity was also observed around the soma of some pyramidal cells. In the rat, positive neurons were also localized in deep cerebellar nuclei, reticular nucleus of the thalamus, and other structures of the midbrain and hindbrain. CA3 region of hippocampus and the external layer of pyriform cortex were characterized by positivity of the neuropil. Immunoelectronmicroscopy showed the antigens in the extracellular space around the neuronal soma, the synaptic elements and the cell processes of the neuropil. The neuronal surface of the soma and of the proximal dendrites were positive, but the pre- and postsynaptic membranes and clefts were negative.

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Crossref · 10.1002/jnr.490290213

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Authors
Bertolotto, A., Rocca, G., Canavese, G., Migheli, A., & Schiffer, D.
Affiliations
University of Turin (IT)
Corresponding author
Bertolotto, Antonio
Venue
Journal of Neuroscience Research
Publisher
Wiley
Published
1991-06
Volume
29
Issue
2
Pages
225-234
ISSN
  • 0360-4012 (ISSN-L)
  • 1097-4547
OpenAlex
W1990239319
Cited by
81
References
32
Citations by year
2022: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2012: 1
FWCI
3.98
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Neuroscience Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
Associazione Italiana per la Ricerca sul Cancro
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
chondroitin sulfate proteoglycans, glycosaminoglycans, central nervous system neurons, interneurons, pyramidal neurons, visual cortex, deep cerebellar nuclei, thalamic reticular nucleus, hippocampal CA3, piriform cortex, extracellular matrix, neuronal localization, immunoelectron microscopy
MeSH terms
Aged, Aged, 80 and over, Animals, Antibodies, Monoclonal, Brain, Cerebellum, Cerebral Cortex, Humans, Middle Aged, Neurons, Chondroitin Sulfate Proteoglycans, Tissue Distribution, Microscopy, Immunoelectron, Rats

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