Immunohistochemical localization of hyaluronic acid in rat and human brain
Abstract
The immunohistochemical localization of hyaluronic acid (HA) was studied in rat and human brain using the monoclonal antibody NDOG1, which specifically recognizes HA. In both rat and human brain, HA-like immunoreactivity formed characteristic coats around neurons in highly selective areas. The staining was abolished by pretreatment of sections with testicular and Streptomyces hyaluronidases, indicating that the staining was specific for HA. In rat brain, positive neurons were located in the cerebral cortex, subiculum, amygdala, thalamic reticular nucleus, nuclei of the inferior colliculus, nuclei of the trapezoid body, and vestibular nuclei. They were also scattered in the hypothalamus, substantia nigra pars reticularis, red nucleus, parabrachial nuclei, brainstem reticular nuclear group, ventral cochlear nucleus, nuclei of lateral lemniscus, and deep cerebellar nuclei. Double immunohistochemical studies showed that many neurons staining for HA were positive for parvalbumin, with minor exceptions in the amygdala and piriform cortex, where some HA-positive neurons were also positive for calbindin-D28k. In the areas studied in human brain, the distribution of HA-positive neurons was virtually identical to that in rat brain. HA-positive neurons were not significantly altered in Alzheimer disease (AD) brain, suggesting that these neurons are resistant to the pathological process of AD.
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The core structure of PNNs consists of chondroitin sulfate proteoglycans of the lectican family, hyaluronan, tenascins, particularly tenascin-R, and link proteins for stabilization.
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From Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses (2019) · cites this paper as [19]
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- Authors
- Yasuhara, O., Akiyama, H., McGeer, E. G., & McGeer, P. L.
- Affiliations
- University of British Columbia (CA)
- Tokyo Institute of Psychiatry (JP)
- Corresponding author
- McGeer, Patrick L.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1994-01
- Volume
- 635
- Issue
- 1-2
- Pages
- 269-282
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2091879191
- Cited by
- 74
- References
- 47
- Citations by year
- 2024: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 4
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
- Keywords
- hyaluronic acid, brain neurons, hyaluronidase, parvalbumin, calbindin-D28k, Alzheimer's disease, neuronal distribution, rat brain, human brain, subiculum, amygdala, thalamic reticular nucleus, cerebellar nuclei, brainstem nuclei
- MeSH terms
- Aged, Aged, 80 and over, Alzheimer Disease, Animals, Brain Chemistry, Brain Stem, Cerebellar Nuclei, Humans, Hyaluronic Acid, Immunoenzyme Techniques, Limbic System, Male, Middle Aged, Rats, Wistar, Rats
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