Cited by 74Proteoglycans and glycosaminoglycans researchNeuroscience and Neuropharmacology ResearchCerebrospinal fluid and hydrocephalus

Immunohistochemical localization of hyaluronic acid in rat and human brain

Yasuhara, Osamu, Akiyama, Haruhiko, McGeer, Edith G., McGeer, Patrick L.·1994·Brain Research

Abstract

The immunohistochemical localization of hyaluronic acid (HA) was studied in rat and human brain using the monoclonal antibody NDOG1, which specifically recognizes HA. In both rat and human brain, HA-like immunoreactivity formed characteristic coats around neurons in highly selective areas. The staining was abolished by pretreatment of sections with testicular and Streptomyces hyaluronidases, indicating that the staining was specific for HA. In rat brain, positive neurons were located in the cerebral cortex, subiculum, amygdala, thalamic reticular nucleus, nuclei of the inferior colliculus, nuclei of the trapezoid body, and vestibular nuclei. They were also scattered in the hypothalamus, substantia nigra pars reticularis, red nucleus, parabrachial nuclei, brainstem reticular nuclear group, ventral cochlear nucleus, nuclei of lateral lemniscus, and deep cerebellar nuclei. Double immunohistochemical studies showed that many neurons staining for HA were positive for parvalbumin, with minor exceptions in the amygdala and piriform cortex, where some HA-positive neurons were also positive for calbindin-D28k. In the areas studied in human brain, the distribution of HA-positive neurons was virtually identical to that in rat brain. HA-positive neurons were not significantly altered in Alzheimer disease (AD) brain, suggesting that these neurons are resistant to the pathological process of AD.

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Crossref · 10.1016/0006-8993(94)91448-6

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Authors
Yasuhara, O., Akiyama, H., McGeer, E. G., & McGeer, P. L.
Affiliations
  • University of British Columbia (CA)
  • Tokyo Institute of Psychiatry (JP)
Corresponding author
McGeer, Patrick L.
Venue
Brain Research
Publisher
Elsevier BV
Published
1994-01
Volume
635
Issue
1-2
Pages
269-282
ISSN
0006-8993 (ISSN-L)
OpenAlex
W2091879191
Cited by
74
References
47
Citations by year
2024: 2 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 4
FWCI
1.52
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
Keywords
hyaluronic acid, brain neurons, hyaluronidase, parvalbumin, calbindin-D28k, Alzheimer's disease, neuronal distribution, rat brain, human brain, subiculum, amygdala, thalamic reticular nucleus, cerebellar nuclei, brainstem nuclei
MeSH terms
Aged, Aged, 80 and over, Alzheimer Disease, Animals, Brain Chemistry, Brain Stem, Cerebellar Nuclei, Humans, Hyaluronic Acid, Immunoenzyme Techniques, Limbic System, Male, Middle Aged, Rats, Wistar, Rats

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