Cited by 106Gut microbiota and healthAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic Diseases

Gut Microbiota Interacts with Markers of Adipose Tissue Browning, Insulin Action and Plasma Acetate in Morbid Obesity

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Moreno‐Navarrete, José María, Serino, Matteo, Blasco‐Baque, Vincent, Azalbert, Vincent, Barton, Richard H., Cardellini, Marina, Latorre, Jèssica, Ortega, Francisco +6 more·2018·Molecular Nutrition & Food Research

Abstract

Scope: To examine the potential relationship among gene expression markers of adipose tissue browning, gut microbiota, and insulin sensitivity in humans. Methods and results: Gut microbiota composition and gene markers of browning are analyzed in subcutaneous (SAT) and visceral (VAT) adipose tissue from morbidly obese subjects (n = 34). Plasma acetate is measured through 1 H NMR and insulin sensitivity using euglycemic hyperinsulinemic clamp. Subjects with insulin resistance show an increase in the relative abundance (RA) of the phyla Bacteroidetes and Proteobacteria while RA of Firmicutes is decreased. In all subjects, Firmicutes RA is negatively correlated with HbA 1c and fasting triglycerides, whereas Proteobacteria RA was negatively correlated with insulin sensitivity. Firmicutes RA is positively associated with markers of brown adipocytes (PRDM16, UCP1, and DIO2) in SAT, but not in VAT. Multivariate regression analysis indicates that Firmicutes RA contributes significantly to SAT PRDM16, UCP1, and DIO2 mRNA variance after controlling for age, BMI, HbA 1c, or insulin sensitivity. Interestingly, Firmicutes RA, specifically those bacteria belonging to the Ruminococcaceae family, is positively associated with plasma acetate levels, which are also linked to SAT PRDM16 mRNA and insulin sensitivity. Conclusion: Gut microbiota composition is linked to adipose tissue browning and insulin action in morbidly obese subjects, possibly through circulating acetate.

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  1. Pedersen, H.K., Gudmundsdottir, V., Nielsen, H.B., Hyotylainen, T., Nielsen, T., Jensen, B.A., Forslund, K., Hildebrand, F., Prifti, E., Falony, G., Le Chatelier, E., Levenez, F., Doré, J., Mattila, I., Plichta, D.R., Pöhö, P., Hellgren, L.I., Arumugam, M., Sunagawa, S., Vieira-Silva, S., Jørgensen, T., Holm, J.B., Trošt, K.; MetaHIT Consortium, Kristiansen, K., Brix, S., Raes, J., Wang, J., Hansen, T., Bork, P., Brunak, S., Oresic, M., Ehrlich, S.D., Pedersen, O., Human gut microbes impact host serum metabolome and insulin sensitivity. Nature 2016, 21, 535:376-381.

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Authors
Moreno‐Navarrete, J. M., Serino, M., Blasco‐Baque, V., Azalbert, V., Barton, R. H., Cardellini, M., Latorre, J., Ortega, F., Sabater‐Masdeu, M., Burcelin, R., Dumas, M., Ricart, W., Federici, M., & Fernández‐Real, J. M.
Affiliations
  • Universitat de Girona (ES)
  • Institut National de la Santé et de la Recherche Médicale (Inserm) Toulouse France
  • Inserm (FR)
  • Imperial College London (GB)
  • University of Rome Tor Vergata (IT)
  • Instituto de Salud Carlos III (ES)
  • Department of Diabetes, Endocrinology and Nutrition Institut d'Investigació Biomèdica de Girona (IdIBGi) CIBEROBN (CB06/03/010) and Instituto de Salud Carlos III (ISCIII) Girona Spain
Corresponding author
  • Moreno‐Navarrete, José María
  • Fernández‐Real, José Manuel
Venue
Molecular Nutrition & Food Research
Publisher
Wiley
Published
2017-12-29
Volume
62
Issue
3
ISSN
  • 1613-4125 (ISSN-L)
  • 1613-4133
OpenAlex
W2766183128
Cited by
106
References
45
Citations by year
2026: 11 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 16 · 2021: 14 · 2020: 21 · 2019: 12 · 2018: 5
FWCI
4.24
Type
journal article
Language
en
Open access
Locations
  • Molecular Nutrition & Food Research (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion)
  • Spiral (Imperial College London) (submittedVersion)
Funders
  • Instituto de Salud Carlos III (PI15/01934)
  • European Regional Development Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
gut microbiota, adipose tissue browning, brown adipocytes, insulin sensitivity, insulin resistance, morbid obesity, subcutaneous adipose tissue, visceral adipose tissue, plasma acetate, Firmicutes, Ruminococcaceae, PRDM16, UCP1, DIO2, Bacteroidetes, Proteobacteria, HbA1c, fasting triglycerides
MeSH terms
Gastrointestinal Microbiome, Uncoupling Protein 1, Acetates, Iodothyronine Deiodinase Type II, Adipose Tissue, Adult, DNA-Binding Proteins, Female, Humans, Insulin Resistance, Iodide Peroxidase, Male, Middle Aged, Obesity, Morbid, Transcription Factors, Biomarkers, Glucose Transporter Type 4, Adipose Tissue, White, Insulin Receptor Substrate Proteins

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