Cited by 769Protein Structure and DynamicsProtein Interaction Studies and Fluorescence AnalysisEnzyme Structure and Function

Hot spots—A review of the protein–protein interface determinant amino‐acid residues

Moreira, Irina S., Fernandes, Pedro A., Ramos, Maria J.·2007·Proteins: Structure, Function, and Bioinformatics

Abstract

Proteins tendency to bind to one another in a highly specific manner forming stable complexes is fundamental to all biological processes. A better understanding of complex formation has many practical applications, which include the rational design of new therapeutic agents, and the analysis of metabolic and signal transduction networks. Alanine‐scanning mutagenesis made possible the detection of the functional epitopes, and demonstrated that most of the protein–protein binding energy is related only to a group of few amino acids at intermolecular protein interfaces: the hot spots. The scope of this review is to summarize all the available information regarding hot spots for a better atomic understanding of their structure and function. The ultimate objective is to improve the rational design of complexes of high affinity and specificity as well as that of small molecules, which can mimic the functional epitopes of the proteic complexes. Proteins 2007. © 2007 Wiley‐Liss, Inc.

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Crossref · 10.1002/prot.21396

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Authors
Moreira, I. S., Fernandes, P. A., & Ramos, M. J.
Affiliations
Universidade do Porto (PT)
Corresponding author
Ramos, Maria J.
Venue
Proteins: Structure, Function, and Bioinformatics
Publisher
Wiley
Published
2007-06
Volume
68
Issue
4
Pages
803-812
ISSN
  • 0887-3585 (ISSN-L)
  • 1097-0134
OpenAlex
W2136206140
Cited by
760
References
118
Citations by year
2026: 28 · 2025: 30 · 2024: 30 · 2023: 38 · 2022: 39 · 2021: 40 · 2020: 41 · 2019: 44 · 2018: 43 · 2017: 36 · 2016: 36 · 2015: 46 · 2014: 49 · 2013: 68 · 2012: 59
FWCI
2.16
Type
journal article
Language
en
Open access
closed
Locations
  • Proteins Structure Function and Bioinformatics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
protein-protein interfaces, amino acid residues, alanine scanning, binding energy, protein complex formation, rational drug design, functional epitopes
MeSH terms
Amino Acids, Binding Sites, Humans, Models, Molecular, Protein Conformation, Proteins, Receptors, Somatotropin, Human Growth Hormone

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