Cited by 164Alzheimer's disease research and treatmentsCell death mechanisms and regulationNeuroinflammation and Neurodegeneration Mechanisms

β-Amyloid Induces Local Neurite Degeneration in Cultured Hippocampal Neurons: Evidence for Neuritic Apoptosis

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Ivins, Kathryn J., Bui, Elizabeth T.N., Cotman, Carl W.·1998·Neurobiology of Disease

Abstract

Many apoptotic insults, including beta-amyloid, cause neuritic degeneration. The possibility that apoptotic insults act directly on neurites was investigated in experiments using compartmented cultures of hippocampal neurons. Neurites in modified Campenot chambers displayed morphological signs of degeneration, including beading or blebbing, when exposed to beta-amyloid. At short time points neurite degeneration was limited to the distal portions of neurites directly exposed to beta-amyloid. Furthermore, annexin V binding detected extracellular exposure of phosphatidylserine in portions of neurites directly exposed to apoptotic insults. Pretreatment of the cultures with zVAD-fmk blocked annexin V binding induced by beta-amyloid and concanavalin A, suggesting that caspase activity was required. Caspase activation was also visualized in neurites locally exposed to apoptotic insults. Together these results show that apoptotic insults cause local neurite degeneration which displays morphological and biochemical characteristics of apoptosis and suggest that neurite degeneration may use mechanisms common to apoptosis.

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Crossref · 10.1006/nbdi.1998.0228

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Authors
Ivins, K. J., Bui, E. T. N., & Cotman, C. W.
Affiliations
University of California, Irvine (US)
Corresponding author
Ivins, Kathryn J.
Venue
Neurobiology of Disease
Publisher
Elsevier BV
Published
1998-11
Volume
5
Issue
5
Pages
365-378
ISSN
0969-9961 (ISSN-L)
OpenAlex
W2012008959
Cited by
164
References
58
Citations by year
2025: 3 · 2024: 2 · 2023: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 2 · 2014: 8 · 2013: 7 · 2012: 8
FWCI
5.52
Type
journal article
Language
en
Open access
Locations
  • Neurobiology of Disease (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health (5r01ag013007-05)
  • University of California, Irvine
  • National Institute on Aging (T32 AG00096, R01-AG13007)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
amyloid-beta, neurite degeneration, hippocampal neurons, phosphatidylserine exposure, annexin V binding, caspase activation, z-VAD-fmk, concanavalin A, Alzheimer's disease
MeSH terms
Animals, Cells, Cultured, Hippocampus, Nerve Degeneration, Neurons, Time Factors, Amyloid beta-Peptides, Neurites, Apoptosis, Annexin A5, Caspases, Rats

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