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Cited by 68Pluripotent Stem Cells ResearchCRISPR and Genetic EngineeringAnimal Genetics and Reproduction

Genetic engineering in organoids

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Teriyapirom, Isaree, Batista-Rocha, Andreia S., Koo, Bon-Kyoung·2021·Journal of Molecular Medicine
DOI 10.1007/s00109-020-02029-zPMC PMC8026415
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Abstract

Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy.

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  • siRNA-mediated knockdown is simple, convenient, economical, and effective in all mammalian somatic cell types.

    “Some of the advantages of siRNA-mediated knockdown are that it is simple, convenient, economical, and effective in all mammalian somatic cell types [45].”

    From Regulation of Enterocyte Brush Border Membrane Primary Na-Absorptive Transporters in Human Intestinal Organoid-Derived Monolayers (2024) · cites this paper as [45]

    The Tools for genetic engineering section (page unavailable) says RNAi, including siRNA-mediated knockdown, is effective in all mammalian somatic cells and requires no prior genetic manipulation. It does not describe siRNA knockdown as simple, convenient, or economical; the Discussion instead notes difficulties with siRNA libraries in screens. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Authors
Teriyapirom, I., Batista-Rocha, A. S., & Koo, B.-K.
Affiliations
Institute of Molecular Biotechnology (AT)
Corresponding author
  • Teriyapirom, Isaree
  • Koo, Bon-Kyoung
ORCID
Venue
Journal of Molecular Medicine
Publisher
Springer Science and Business Media LLC
Published
2021-01-18
Volume
99
Issue
4
Pages
555-568
ISSN
  • 0946-2716 (ISSN-L)
  • 1432-1440
DOI
10.1007/s00109-020-02029-z
PMC
PMC8026415
OpenAlex
W3124541903
Cited by
68
References
27
Citations by year
2026: 8 · 2025: 14 · 2024: 17 · 2023: 11 · 2022: 12 · 2021: 6
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Molecular Medicine (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Human Frontier Science Program
  • Österreichischen Akademie der Wissenschaften
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords
organoids, genetic engineering, pluripotent stem cells, organoid differentiation, disease modeling, developmental modeling, human organoids
MeSH terms
Cellular Reprogramming Techniques, Gene Editing, Animals, Brain, Genetic Engineering, Humans, Kidney, Liver, Mammary Glands, Animal, Organoids, Gene Transfer Techniques, Animals, Genetically Modified, Gastrointestinal Tract, Mice, Adult Stem Cells, Induced Pluripotent Stem Cells, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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