Cited by 1,000Neurogenesis and neuroplasticity mechanismsPluripotent Stem Cells ResearchDevelopmental Biology and Gene Regulation

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

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Kadoshima, Taisuke, Sakaguchi, Hideya, Nakano, Tokushige, Soen, Mika, Ando, Satoshi, Eiraku, Mototsugu, Sasai, Yoshiki·2013·Proceedings of the National Academy of Sciences

Abstract

Significance: Using 3D culture of human ES cells, we show new self-organizing aspects of human corticogenesis: spontaneous development of intracortical polarity, curving morphology, and complex zone separations. Moreover, this culture generates species-specific progenitors, outer radial glia, which are abundantly present in the human, but not mouse, neocortex. Our study suggests an unexpectedly wide range of self-organizing events that are driven by internal programs in human neocortex development.

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Crossref · 10.1073/pnas.1315710110

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  • Production of radial glial cells, intermediate progenitors, and deep- and superficial-layer neurons in an ordered temporal fashion has been reported by studies using all current protocols.

    “The production of radial glial cells, intermediate progenitors, and deep- and superficial-layer neurons in an ordered temporal fashion has been reported by studies using all of the protocols currently in use2,3,5,25.”
  • These protocols generated organoids with a dorsalized neuroepithelium that reproduced certain structural and cell-behaviour aspects of cortical development.

    “Both of these protocols generated organoids containing a dorsalized neuroepithelium that reproduced certain aspects of cortical development, both structurally and in terms of cell behaviour.”
  • The developmental timing of cortical neurogenesis appears conserved in vitro and has been demonstrated with both suspension and adherent differentiation methods.

    “Interestingly, the developmental timing of cortical neurogenesis appears to be conserved in vitro and has been demonstrated with both suspension and adherent differentiation methods2,3,5,39.”

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Research works printed but not linked: 3

  1. Sheppard AM, Pearlman AL (1997) Abnormal reorganization of preplate neurons and their associated extracellular matrix: An early manifestation of altered neocortical development in the reeler mutant mouse. J Comp Neurol 378(2):173-179.
  2. Bayer SA, Altman J (2005) Atlas of Human Central Nervous System Development, Volume 3: The Human Brain During the Second Trimester (CRC Press, Boca Raton, FL).
  3. Bayer SA, Altman J (2004) Atlas of Human Central Nervous System Development, Volume 2: The Human Brain During the Third Trimester (CRC Press, Boca Raton, FL).

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Authors
Kadoshima, T., Sakaguchi, H., Nakano, T., Soen, M., Ando, S., Eiraku, M., & Sasai, Y.
Affiliations
  • Kyoto University (JP)
  • Laboratory of Organogenesis and Neurogenesis and
  • RIKEN (JP)
Venue
Proceedings of the National Academy of Sciences
Published
2013-11-25
Volume
110
Issue
50
Pages
20284-20289
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2051785391
Cited by
999
References
33
Citations by year
2026: 62 · 2025: 99 · 2024: 90 · 2023: 84 · 2022: 122 · 2021: 113 · 2020: 113 · 2019: 94 · 2018: 66 · 2017: 66 · 2016: 44 · 2015: 30 · 2014: 16
FWCI
11.13
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Kyoto University Research Information Repository (Kyoto University) (submittedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Japan Society for the Promotion of Science (24680037)
  • Japan Science and Technology Agency
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
human embryonic stem cells, self-organization, axial polarity, subplate, cortical plate, outer subventricular zone, outer radial glia, 3D cell culture, cortical morphogenesis
MeSH terms
Amides, Collagen, Drug Combinations, Humans, Laminin, Neuroglia, Proteoglycans, Pyridines, Species Specificity, Cell Polarity, Cell Culture Techniques, Neocortex, Organogenesis, Embryonic Stem Cells

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