Plasma NEFA storage in adipose tissue in the postprandial state: sex-related and regional differences
Open accessAbstract
Aims/hypothesis We recently reported that a small fraction of circulating NEFA is stored through direct uptake in subcutaneous fat in postabsorptive humans in vivo and that this pathway may favour lower-body fat distribution in women. Here, we examined sex-related and regional differences in storage of plasma NEFA in subcutaneous adipose tissue during postprandial conditions.Methods At 1 h after lunch, men and women of normal weight received an intravenous bolus of approximately 1.66 MBq [1-(14)C]oleate followed by timed subcutaneous fat biopsies. The preceding breakfast was either a normal- or high-fat meal; the high-fat breakfast was used to create postprandial oleate concentrations in the postabsorptive range.Results Storage of the NEFA tracer in adipose tissue (dpm/g lipid) was greater in women; in both sexes abdominal fat stored tracer more avidly than femoral fat. A greater fraction of the administered tracer was stored in whole body subcutaneous fat of women than in that of men (27 +/- 3 vs 8 +/- 1%, respectively, p < 0.0001). No significant differences in tracer storage were observed between participants consuming the high- vs normal-fat breakfast.Conclusions/interpretation Postprandial NEFA storage in subcutaneous fat through direct uptake accounts for approximately 25% of NEFA disposal in women, but for <10% in men in a wide range of circulating NEFA concentrations. It is greater in the upper- than lower-body subcutaneous fat, favouring upper-body fat accumulation in both sexes.
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Two months of transdermal testosterone treatment in non-obese males did not affect femoral meal fat uptake but decreased abdominal subcutaneous LPL activity and meal fat storage.
“In contrast, 2 months of transdermal testosterone treatment given to non-obese males did not affect femoral meal fat uptake (147), but did decrease abdominal subcutaneous LPL activity and meal fat storage (147).”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [147]
The Introduction and Study Protocol describe a postprandial NEFA-tracer study in normal-weight men and women, not a testosterone-treatment study. The Results report regional tracer storage, but no passage found for transdermal testosterone, a two-month intervention, or treatment-related changes in LPL activity or meal-fat storage. (LLM, conf=0.76)
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- Authors
- Koutsari, C., Snozek, C. L. H., & Jensen, M. D.
- Affiliations
- Mayo Clinic (US)
- Corresponding author
- Jensen, M. D.
- Venue
- Diabetologia
- Published
- 2008-08-19
- Volume
- 51
- Issue
- 11
- Pages
- 2041-2048
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- PMC
- PMC2637373
- OpenAlex
- W2087974950
- Cited by
- 47
- References
- 20
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 3 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 5 · 2013: 2 · 2012: 4
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Diabetologia (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Mayo Clinic
- Mayo Foundation for Medical Education and Research
- Deutsches Krebsforschungszentrum
- U.S. Public Health Service (DK45343, DK-40484, DK-50456, R-00585)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK 40484, P30DK050456, DK 45343, DK50456, R37DK040484, R01DK045343, R01DK040484)
- National Center for Research Resources (M01RR000585)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- free fatty acids, subcutaneous adipose tissue, postprandial state, sex differences, regional variation, abdominal fat, high-fat meal, body fat distribution
- MeSH terms
- Adipose Tissue, Adult, Blood Glucose, Energy Intake, Fatty Acids, Nonesterified, Female, Humans, Insulin, Lipoproteins, VLDL, Male, Reference Values, Sex Characteristics, Premenopause, Postprandial Period
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