Cited by 158Plant Genetic and Mutation StudiesZebrafish Biomedical Research ApplicationsGenetic Mapping and Diversity in Plants and Animals

Behavior and mutagenesis screens: The importance of baseline analysis of inbred strains

Tarantino, Lisa M., Gould, Thomas J., Druhan, Jonathan P., Bucan, Maja·2000·Mammalian Genome

Abstract

Random mutagenesis as a means of identifying the function of genes has been used extensively in a variety of model organisms. Until recently it has been used primarily in the identification of single-gene traits that cause visible and developmental mutations. However, this genetic approach also has the power to identify genes that control complex biological systems such as behavior. Mutagenesis screens for behavioral mutations require careful consideration of many factors, including choice of both assays and background strains for use in mutagenesis and subsequent mapping of the affected gene or genes. This paper describes behavioral assays for monitoring motor coordination on the accelerating rotarod, anxiety-related behaviors in the elevated zero maze and sensorimotor reactivity, gating, and habituation of acoustic startle. These five physiological or neurological behaviors can represent potential endophenotypes for a variety of neurological and psychiatric disorders. The significant degree of strain- and sex-specific differences in the performance of four inbred strains of mice (C57BL/6J, C3HeB/FeJ, DBA/2J, and 129/SvlmJ) in these behavioral assays illustrates the importance of performing baseline analysis prior to behavioral mutagenesis screens and genetic mapping of selected mutations.

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Crossref · 10.1007/s003350010107

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  • Parental lines of the 129SvJ-C57BL/6J F1 hybrid mice show differences in motor function, anxiety-related behavior, and sensorimotor responsivity, which may contribute to differential effects of partial nAChR agonists on depression-like behavior.

    “previous research showed that the parental lines of the 129SvJ-C57BL/6J F1 hybrid mice show differences in motor function, anxiety-related behavior, and sensorimotor responsivity, which may contribute to the differential effects of partial nAChR agonists on depression-like behavior (Tarantino, Gould, Druhan, & Bucan, 2000).”

    From Nicotine addiction and psychiatric disorders (2015) · cites this paper as Tarantino et al., 2000

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Authors
Tarantino, L. M., Gould, T. J., Druhan, J. P., & Bucan, M.
Affiliations
University of Pennsylvania (US)
Venue
Mammalian Genome
Published
2000-07
Volume
11
Issue
7
Pages
555-564
ISSN
  • 0938-8990 (ISSN-L)
  • 1432-1777
OpenAlex
W1964871934
Cited by
158
References
52
Citations by year
2026: 2 · 2025: 2 · 2024: 3 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 1 · 2013: 5 · 2012: 6
FWCI
24.62
Type
journal article
Language
en
Open access
closed
Locations
  • Mammalian Genome (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01mh057855-04, 7r01da010088-03, 5r01ar045325-04)
  • National Institute of Mental Health (MH 57855)
  • National Institute on Drug Abuse (DA10088)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR45325-02)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
Keywords
inbred mouse strains, baseline behavior, strain differences, sex differences, motor coordination, rotarod test, anxiety, elevated zero maze, acoustic startle response, habituation, endophenotypes, gene mapping, C57BL/6J mice, DBA/2J, 129/Sv mice
MeSH terms
Animals, Behavior, Animal, Chromosome Mapping, Female, Genetic Testing, Male, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred DBA, Mice, Inbred Strains, Motor Activity, Sex Factors, Species Specificity, Reflex, Startle, Time Factors, Mutagenesis, Maze Learning, Mice

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