A Marine Strain of Flavobacteriaceae Utilizes Brown Seaweed Fucoidan
Abstract
Fucoidan, a mixture of sulfated fucose-containing polysaccharides, was prepared from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) with a yield of about 3.8% dry weight. To isolate enzymes that degrade fucoidan, we first screened marine bacteria for their ability to utilize fucoidan, and isolated one strain of Flavobacteriaceae from seawater that could do this. Phylogenetic analysis of the 16S ribosomal DNA sequence suggested that this strain appeared to belong to a new genus, and was tentatively named Fucobacter marina. The strain utilized L-fucose (17%), D-mannose (91%), D-galactose (46%), and D-glucuronic acid (66%) in the fucoidan from K. crassifolia. The strain partially utilized fucoidan from 2 other seaweeds that belong to the order Laminariales, Undaria pinnatifida (10%) and Lessonia nigrescens (48%).
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Another type of fucoidanase catalyses glycosidic bonds (both α(1→3)- and α(1→4)-bonds) in the fucose-backbone via random endo-type hydrolysis.
“the other type of fucoidanase catalyses the glycosidic bonds (both a(1A3)-, a(1A4)-bonds) in the fucose-back bone via random endo-type hydrolysis”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [34]
One type of fucoidanase catalyses cleavage of small, low molecular weight oligosaccharides from the end of fucoidan backbone chains.
“one type of fucoidanase catalyses the cleavage of small, low molecular weight oligosaccharides from the end of the fucoidan backbone chains”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [34]
α-L-fucosidase only catalyses cleavage of α-L-fucosyl linkages at the nonreducing termini, releasing fucose from the fucose-backbone structure in fucoidans.
“a-L-fucosidase only catalyses the cleavage the a-L-fucosyl linkages at the nonreducing termini, releasing fucose from the fucose-backbone structure in fucoidans (Enzyme type 3, Fig. 1).”
From Fucoidans from brown seaweeds: an update on structures, extraction techniques and use of enzymes as tools for structural elucidation (2013) · cites this paper as [34]
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Ale, M. T., & Meyer, A. S. (2013) · RSC Adv. · doi:10.1039/c3ra23373a · Cited by 381 · see citing works
Structure, biological activity, and enzymatic transformation of fucoidans from the brown seaweeds
Kusaykin, M., Bakunina, I., Sova, V., Ermakova, S., Kuznetsova, T., Besednova, N., Zaporozhets, T., & Zvyagintseva, T. (2008) · Biotechnology Journal · doi:10.1002/biot.200700054 · Cited by 197 · see citing works
Holtkamp, A. D., Kelly, S., Ulber, R., & Lang, S. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1790-x · Cited by 175 · see citing works
Ale, M. T., Mikkelsen, J. D., & Meyer, A. S. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-011-9690-3 · Cited by 113 · see citing works
Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae
Descamps, V., Colin, S., Lahaye, M., Jam, M., Richard, C., Potin, P., Barbeyron, T., Yvin, J.-C., & Kloareg, B. (2006) · Marine Biotechnology · doi:10.1007/s10126-005-5107-0 · Cited by 108 · see citing works
Shimanaka, K., Ikai, K., Kato, I., Sakai, T., & Ishizuka, K. (2003) · Marine Biotechnology · doi:10.1007/s10126-002-0107-9 · Cited by 83 · see citing works
Self-citationIsolation and Characterization of a Fucoidan-Degrading Marine Bacterial Strain and Its Fucoidanase
Sakai, T., Kawai, T., & Kato, I. (2004) · Marine Biotechnology · doi:10.1007/s10126-003-0033-5 · Cited by 69 · see citing works
Self-citation
- Authors
- Sakai, T., Kimura, H., & Kato, I.
- Affiliations
- Takara Shuzo Co., Ltd. (Japan) (JP)
- ORCID
- Sakai, Takeshi
- Venue
- Marine Biotechnology
- Published
- 2002-09-01
- Volume
- 4
- Issue
- 4
- Pages
- 399-405
- ISSN
- 1436-2228 (ISSN-L)
- OpenAlex
- W1974819109
- Cited by
- 75
- References
- 0
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 5 · 2019: 2 · 2018: 7 · 2017: 3 · 2016: 1 · 2015: 4 · 2014: 1 · 2013: 3 · 2012: 4
- FWCI
- 3.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Marine Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- fucoidan, Flavobacteriaceae, brown seaweed polysaccharides, polysaccharide degradation, marine bacteria, novel bacterial genus, kelp, Undaria pinnatifida, Lessonia nigrescens
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